Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells
<p>Abstract</p> <p>Background</p> <p>The function of the cortical microtubules, composed of αβ-tubulin heterodimers, is linked to their organizational state which is subject to spatial and temporal modulation by environmental cues. The role of tubulin posttranslational...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-02-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/29 |
| _version_ | 1818358988430901248 |
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| author | Sulimenko Tetyana Sulimenko Vadym Nyporko Alexey Sheremet Yarina Yemets Alla Blume Yaroslav Dráber Pavel |
| author_facet | Sulimenko Tetyana Sulimenko Vadym Nyporko Alexey Sheremet Yarina Yemets Alla Blume Yaroslav Dráber Pavel |
| author_sort | Sulimenko Tetyana |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The function of the cortical microtubules, composed of αβ-tubulin heterodimers, is linked to their organizational state which is subject to spatial and temporal modulation by environmental cues. The role of tubulin posttranslational modifications in these processes is largely unknown. Although antibodies against small tubulin regions represent useful tool for studying molecular configuration of microtubules, data on the exposure of tubulin epitopes on plant microtubules are still limited.</p> <p>Results</p> <p>Using homology modeling we have generated an <it>Arabidopsis thaliana </it>microtubule protofilament model that served for the prediction of surface exposure of five β-tubulin epitopes as well as tyrosine residues. Peptide scans newly disclosed the position of epitopes detected by antibodies 18D6 (β1-10), TUB2.1 (β426-435) and TU-14 (β436-445). Experimental verification of the results by immunofluorescence microscopy revealed that the exposure of epitopes depended on the mode of fixation. Moreover, homology modeling showed that only tyrosines in the C-terminal region of β-tubulins (behind β425) were exposed on the microtubule external side. Immunofluorescence microscopy revealed tyrosine phosphorylation of microtubules in plant cells, implying that β-tubulins could be one of the targets for tyrosine kinases.</p> <p>Conclusions</p> <p>We predicted surface exposure of five β-tubulin epitopes, as well as tyrosine residues, on the surface of <it>A. thaliana </it>microtubule protofilament model, and validated the obtained results by immunofluorescence microscopy on cortical microtubules in cells.</p> <p>The results suggest that prediction of epitope exposure on microtubules by means of homology modeling combined with site-directed antibodies can contribute to a better understanding of the interactions of plant microtubules with associated proteins.</p> |
| first_indexed | 2024-12-13T20:37:45Z |
| format | Article |
| id | doaj.art-fd88c496e6d14aeead46a5d18311a4c1 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-13T20:37:45Z |
| publishDate | 2010-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-fd88c496e6d14aeead46a5d18311a4c12022-12-21T23:32:14ZengBMCBMC Plant Biology1471-22292010-02-011012910.1186/1471-2229-10-29Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cellsSulimenko TetyanaSulimenko VadymNyporko AlexeySheremet YarinaYemets AllaBlume YaroslavDráber Pavel<p>Abstract</p> <p>Background</p> <p>The function of the cortical microtubules, composed of αβ-tubulin heterodimers, is linked to their organizational state which is subject to spatial and temporal modulation by environmental cues. The role of tubulin posttranslational modifications in these processes is largely unknown. Although antibodies against small tubulin regions represent useful tool for studying molecular configuration of microtubules, data on the exposure of tubulin epitopes on plant microtubules are still limited.</p> <p>Results</p> <p>Using homology modeling we have generated an <it>Arabidopsis thaliana </it>microtubule protofilament model that served for the prediction of surface exposure of five β-tubulin epitopes as well as tyrosine residues. Peptide scans newly disclosed the position of epitopes detected by antibodies 18D6 (β1-10), TUB2.1 (β426-435) and TU-14 (β436-445). Experimental verification of the results by immunofluorescence microscopy revealed that the exposure of epitopes depended on the mode of fixation. Moreover, homology modeling showed that only tyrosines in the C-terminal region of β-tubulins (behind β425) were exposed on the microtubule external side. Immunofluorescence microscopy revealed tyrosine phosphorylation of microtubules in plant cells, implying that β-tubulins could be one of the targets for tyrosine kinases.</p> <p>Conclusions</p> <p>We predicted surface exposure of five β-tubulin epitopes, as well as tyrosine residues, on the surface of <it>A. thaliana </it>microtubule protofilament model, and validated the obtained results by immunofluorescence microscopy on cortical microtubules in cells.</p> <p>The results suggest that prediction of epitope exposure on microtubules by means of homology modeling combined with site-directed antibodies can contribute to a better understanding of the interactions of plant microtubules with associated proteins.</p>http://www.biomedcentral.com/1471-2229/10/29 |
| spellingShingle | Sulimenko Tetyana Sulimenko Vadym Nyporko Alexey Sheremet Yarina Yemets Alla Blume Yaroslav Dráber Pavel Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells BMC Plant Biology |
| title | Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells |
| title_full | Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells |
| title_fullStr | Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells |
| title_full_unstemmed | Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells |
| title_short | Exposure of beta-tubulin regions defined by antibodies on an <it>Arabidopsis thaliana </it>microtubule protofilament model and in the cells |
| title_sort | exposure of beta tubulin regions defined by antibodies on an it arabidopsis thaliana it microtubule protofilament model and in the cells |
| url | http://www.biomedcentral.com/1471-2229/10/29 |
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