Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms
The significance of <em>Aeromonas hydrophila</em> concerning hemorrhagic septicemia in aquaculture farms production in Duhok province, Iraq was investigated. Antibiotic-resistant profiles of isolates were also investigated with 8 antibiotics. Bacterial isolates were identified by using m...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2021-02-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://vetmedmosul.com/article_167615_b1fa4f9278a396fc1faed14e4d9e61bf.pdf |
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| author | Zanan Taha Shaaban Sadiq Warheen Khalil Kaheen Muhammad-Ali Hayam Yosif Hozan Shamil |
| author_facet | Zanan Taha Shaaban Sadiq Warheen Khalil Kaheen Muhammad-Ali Hayam Yosif Hozan Shamil |
| author_sort | Zanan Taha |
| collection | DOAJ |
| description | The significance of <em>Aeromonas hydrophila</em> concerning hemorrhagic septicemia in aquaculture farms production in Duhok province, Iraq was investigated. Antibiotic-resistant profiles of isolates were also investigated with 8 antibiotics. Bacterial isolates were identified by using morphological and biochemical tests and confirmed molecularly by amplification of <em>gcat</em> gene. Out of 25 examined fish, only 19 fish were harbored <em>A. hydrophila</em>. Twenty-four <em>A. hydrophila</em> strains were isolates from 100 organ samples<em>. </em>Ninety-six percentages of the isolates were resistant to each of the imipenem and gentamicin, followed by doxycycline 92%, ciprofloxacin and trimethoprim-sulfamethoxazole 88%, norfloxacin 58% and ceftriaxone 33%. None were resistant to levofloxacin. Eighty-eight percentages were multiple antibiotics resistant. The high isolation rate of <em>A. hydrophila</em> in our study indicates that this species was the major cause of the outbreak in hemorrhagic septicemia’s cases in our area affecting carp farms and the high rate of resistance should be considered as these isolates can serve as a resistance source for human being during food series and make a great challenge for their therapeutic opportunity. |
| first_indexed | 2024-12-20T00:32:19Z |
| format | Article |
| id | doaj.art-fd8f8088b59d435eb7abaa4159f2c986 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-20T00:32:19Z |
| publishDate | 2021-02-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-fd8f8088b59d435eb7abaa4159f2c9862022-12-21T19:59:54ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552021-02-0135237538010.33899/ijvs.2020.126876.1405167615Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farmsZanan Taha0Shaaban Sadiq1Warheen Khalil2Kaheen Muhammad-Ali3Hayam Yosif4Hozan Shamil5Department of Pathology and Microbiology, College of Veterinary Medicine, Duhok University, Iraq. Ph.D. in Microbiology/ BacteriologyDepartment of Pathology and Microbiology, College of Veterinary Medicine, Duhok University, Iraq.Department of Medicine and Surgery, College of Veterinary Medicine, Duhok University, IraqDepartment of Medicine and Surgery, College of Veterinary Medicine, Duhok University, Iraq.Department of Medicine and Surgery, College of Veterinary Medicine, Duhok University, Iraq.Department of Medicine and Surgery, College of Veterinary Medicine, Duhok University, Iraq.The significance of <em>Aeromonas hydrophila</em> concerning hemorrhagic septicemia in aquaculture farms production in Duhok province, Iraq was investigated. Antibiotic-resistant profiles of isolates were also investigated with 8 antibiotics. Bacterial isolates were identified by using morphological and biochemical tests and confirmed molecularly by amplification of <em>gcat</em> gene. Out of 25 examined fish, only 19 fish were harbored <em>A. hydrophila</em>. Twenty-four <em>A. hydrophila</em> strains were isolates from 100 organ samples<em>. </em>Ninety-six percentages of the isolates were resistant to each of the imipenem and gentamicin, followed by doxycycline 92%, ciprofloxacin and trimethoprim-sulfamethoxazole 88%, norfloxacin 58% and ceftriaxone 33%. None were resistant to levofloxacin. Eighty-eight percentages were multiple antibiotics resistant. The high isolation rate of <em>A. hydrophila</em> in our study indicates that this species was the major cause of the outbreak in hemorrhagic septicemia’s cases in our area affecting carp farms and the high rate of resistance should be considered as these isolates can serve as a resistance source for human being during food series and make a great challenge for their therapeutic opportunity.https://vetmedmosul.com/article_167615_b1fa4f9278a396fc1faed14e4d9e61bf.pdfaeromonas hydrophilahemorrhagic septicemiamolecular detectionantimicrobial resistance |
| spellingShingle | Zanan Taha Shaaban Sadiq Warheen Khalil Kaheen Muhammad-Ali Hayam Yosif Hozan Shamil Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms Iraqi Journal of Veterinary Sciences aeromonas hydrophila hemorrhagic septicemia molecular detection antimicrobial resistance |
| title | Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms |
| title_full | Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms |
| title_fullStr | Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms |
| title_full_unstemmed | Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms |
| title_short | Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms |
| title_sort | investigation of gcat gene and antibiotic resistance pattern of aeromonas hydrophila isolated from hemorrhagic septicemia s cases in fish farms |
| topic | aeromonas hydrophila hemorrhagic septicemia molecular detection antimicrobial resistance |
| url | https://vetmedmosul.com/article_167615_b1fa4f9278a396fc1faed14e4d9e61bf.pdf |
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