Enterohepatic circulation in hamsters with an extracorporeal bile duct.
The present study describes a novel technique for investigations of the enterohepatic circulation in the hamster with an extracorporeal bile duct that allows long-term bile collection in the free-moving animal. The animals recovered for 7 days after the operation before the external loop was cut and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1992-09-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520405528 |
| _version_ | 1818833149954621440 |
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| author | M Fuchs J Scheibner E Hörmann G Tauber EF Stange |
| author_facet | M Fuchs J Scheibner E Hörmann G Tauber EF Stange |
| author_sort | M Fuchs |
| collection | DOAJ |
| description | The present study describes a novel technique for investigations of the enterohepatic circulation in the hamster with an extracorporeal bile duct that allows long-term bile collection in the free-moving animal. The animals recovered for 7 days after the operation before the external loop was cut and bile was collected over a period of 78 h. Under these optimal conditions, initial bile flow (651 +/- 89 microliters per 100 g.h-1) and the secretion rates of biliary lipids were several-fold higher than reported in an earlier study using the acute fistula hamster. Biliary cholesterol secretion amounted to 369 +/- 32 nmol per 100 g.h-1, phospholipid secretion was 2.6 +/- 0.3 mumol per 100 g.h-1, and total bile acid secretion was 31.9 +/- 2.2 mumol per 100 g.h-1. A clearcut diurnal rhythm was demonstrated for bile flow and all biliary constituents. After 9 h the depletion of the bile acid pool was complete and cholic acid synthesis derepressed 1.4-fold from a basal rate of 818 nmol per 100 g.h-1, whereas the derepression of chenodeoxycholic acid synthesis was even less pronounced. Biliary cholesterol output increased 2.2-fold, but the phospholipid secretion was constant during the full experiment. It may be concluded that the technique of an extracorporeal bile duct in the free-moving animal allows studies of bile secretion under optimal conditions. Most likely the bile secretion rates given above approach the physiological rates in the hamster. |
| first_indexed | 2024-12-19T02:14:20Z |
| format | Article |
| id | doaj.art-fd9862e1094e4e989a5bc501109426c2 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T02:14:20Z |
| publishDate | 1992-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fd9862e1094e4e989a5bc501109426c22022-12-21T20:40:30ZengElsevierJournal of Lipid Research0022-22751992-09-0133913831391Enterohepatic circulation in hamsters with an extracorporeal bile duct.M Fuchs0J Scheibner1E Hörmann2G Tauber3EF Stange4Department of Internal Medicine II, University of Ulm, Germany.Department of Internal Medicine II, University of Ulm, Germany.Department of Internal Medicine II, University of Ulm, Germany.Department of Internal Medicine II, University of Ulm, Germany.Department of Internal Medicine II, University of Ulm, Germany.The present study describes a novel technique for investigations of the enterohepatic circulation in the hamster with an extracorporeal bile duct that allows long-term bile collection in the free-moving animal. The animals recovered for 7 days after the operation before the external loop was cut and bile was collected over a period of 78 h. Under these optimal conditions, initial bile flow (651 +/- 89 microliters per 100 g.h-1) and the secretion rates of biliary lipids were several-fold higher than reported in an earlier study using the acute fistula hamster. Biliary cholesterol secretion amounted to 369 +/- 32 nmol per 100 g.h-1, phospholipid secretion was 2.6 +/- 0.3 mumol per 100 g.h-1, and total bile acid secretion was 31.9 +/- 2.2 mumol per 100 g.h-1. A clearcut diurnal rhythm was demonstrated for bile flow and all biliary constituents. After 9 h the depletion of the bile acid pool was complete and cholic acid synthesis derepressed 1.4-fold from a basal rate of 818 nmol per 100 g.h-1, whereas the derepression of chenodeoxycholic acid synthesis was even less pronounced. Biliary cholesterol output increased 2.2-fold, but the phospholipid secretion was constant during the full experiment. It may be concluded that the technique of an extracorporeal bile duct in the free-moving animal allows studies of bile secretion under optimal conditions. Most likely the bile secretion rates given above approach the physiological rates in the hamster.http://www.sciencedirect.com/science/article/pii/S0022227520405528 |
| spellingShingle | M Fuchs J Scheibner E Hörmann G Tauber EF Stange Enterohepatic circulation in hamsters with an extracorporeal bile duct. Journal of Lipid Research |
| title | Enterohepatic circulation in hamsters with an extracorporeal bile duct. |
| title_full | Enterohepatic circulation in hamsters with an extracorporeal bile duct. |
| title_fullStr | Enterohepatic circulation in hamsters with an extracorporeal bile duct. |
| title_full_unstemmed | Enterohepatic circulation in hamsters with an extracorporeal bile duct. |
| title_short | Enterohepatic circulation in hamsters with an extracorporeal bile duct. |
| title_sort | enterohepatic circulation in hamsters with an extracorporeal bile duct |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520405528 |
| work_keys_str_mv | AT mfuchs enterohepaticcirculationinhamsterswithanextracorporealbileduct AT jscheibner enterohepaticcirculationinhamsterswithanextracorporealbileduct AT ehormann enterohepaticcirculationinhamsterswithanextracorporealbileduct AT gtauber enterohepaticcirculationinhamsterswithanextracorporealbileduct AT efstange enterohepaticcirculationinhamsterswithanextracorporealbileduct |