Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions
Sesame meal is a by-product obtained from oil extraction. We investigated the characteristics and antioxidant activities of a sesame protein hydrolysate (SPH–B), as well as its peptide fractions. Four peptide fractions (F1; >100 kDa, F2; 10–100 kDa, F3; 1–10 kDa, and F4; <1 kDa) of SPH-B were...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024039227 |
| _version_ | 1827294024536424448 |
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| author | Rodjana Noptana David Julian McClements Lynne A. McLandsborough Ekasit Onsaard |
| author_facet | Rodjana Noptana David Julian McClements Lynne A. McLandsborough Ekasit Onsaard |
| author_sort | Rodjana Noptana |
| collection | DOAJ |
| description | Sesame meal is a by-product obtained from oil extraction. We investigated the characteristics and antioxidant activities of a sesame protein hydrolysate (SPH–B), as well as its peptide fractions. Four peptide fractions (F1; >100 kDa, F2; 10–100 kDa, F3; 1–10 kDa, and F4; <1 kDa) of SPH-B were prepared. The characteristics and antioxidant properties of SPH-B and its peptide fractions were evaluated. Sesame protein (SP) contained protein fractions with molecular weights ranging from 10 to 44 kDa, whereas SPH-B had peptide fractions ranging from 8 to 44 kDa. The peptide fractions had molecular weight ranging from 7 to 10 kDa. The four peptide fractions had a higher α-helix content and lower surface hydrophobicity than SPH-B and SP. They exhibited better antioxidant properties, with higher ABTS and DPPH radical scavenging activities, higher metal chelating activity, and greater inhibition of linoleic acid peroxidation, suggesting that sesame peptide fractions can use as plant-based functional ingredients and potentially health-promoting properties. |
| first_indexed | 2024-04-24T13:49:59Z |
| format | Article |
| id | doaj.art-fd9864c4d34e444b9efc9dc698a4f11f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:49:59Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-fd9864c4d34e444b9efc9dc698a4f11f2024-04-04T05:06:16ZengElsevierHeliyon2405-84402024-03-01106e27891Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractionsRodjana Noptana0David Julian McClements1Lynne A. McLandsborough2Ekasit Onsaard3Indigenous Food Research and Industrial Development Center, Faculty of Agriculture, Ubon Ratchathami University, Warinchumrap, Ubon Ratchathani, 34190, ThailandBiopolymers & Colloids Research Laboratory, Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USAFood Microbiology, Department of Food Science, University of Massachusetts, Amherst, MA, 01003, USAIndigenous Food Research and Industrial Development Center, Faculty of Agriculture, Ubon Ratchathami University, Warinchumrap, Ubon Ratchathani, 34190, Thailand; Corresponding author. Indigenous Food Research and Industrial Development Center, Faculty of Agriculture, Ubon Ratchathani University, 34190, Thailand.Sesame meal is a by-product obtained from oil extraction. We investigated the characteristics and antioxidant activities of a sesame protein hydrolysate (SPH–B), as well as its peptide fractions. Four peptide fractions (F1; >100 kDa, F2; 10–100 kDa, F3; 1–10 kDa, and F4; <1 kDa) of SPH-B were prepared. The characteristics and antioxidant properties of SPH-B and its peptide fractions were evaluated. Sesame protein (SP) contained protein fractions with molecular weights ranging from 10 to 44 kDa, whereas SPH-B had peptide fractions ranging from 8 to 44 kDa. The peptide fractions had molecular weight ranging from 7 to 10 kDa. The four peptide fractions had a higher α-helix content and lower surface hydrophobicity than SPH-B and SP. They exhibited better antioxidant properties, with higher ABTS and DPPH radical scavenging activities, higher metal chelating activity, and greater inhibition of linoleic acid peroxidation, suggesting that sesame peptide fractions can use as plant-based functional ingredients and potentially health-promoting properties.http://www.sciencedirect.com/science/article/pii/S2405844024039227Sesame proteinPeptide fractionAntioxidant activityPlant-based proteinsProtease activity |
| spellingShingle | Rodjana Noptana David Julian McClements Lynne A. McLandsborough Ekasit Onsaard Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions Heliyon Sesame protein Peptide fraction Antioxidant activity Plant-based proteins Protease activity |
| title | Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| title_full | Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| title_fullStr | Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| title_full_unstemmed | Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| title_short | Comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| title_sort | comparison of characteristics and antioxidant activities of sesame protein hydrolysates and their fractions |
| topic | Sesame protein Peptide fraction Antioxidant activity Plant-based proteins Protease activity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024039227 |
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