Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes
Proteins, lipids, and carbohydrates from the harmful algal bloom (HAB)-causing organism <i>Pyrodinium bahamense</i> were characterized to obtain insights into the biochemical processes in this environmentally relevant dinoflagellate. Shotgun proteomics using label-free quantitation follo...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13332 |
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| author | Bryan John J. Subong Zabrina Bernice L. Malto Arturo O. Lluisma Rhodora V. Azanza Lilibeth A. Salvador-Reyes |
| author_facet | Bryan John J. Subong Zabrina Bernice L. Malto Arturo O. Lluisma Rhodora V. Azanza Lilibeth A. Salvador-Reyes |
| author_sort | Bryan John J. Subong |
| collection | DOAJ |
| description | Proteins, lipids, and carbohydrates from the harmful algal bloom (HAB)-causing organism <i>Pyrodinium bahamense</i> were characterized to obtain insights into the biochemical processes in this environmentally relevant dinoflagellate. Shotgun proteomics using label-free quantitation followed by proteome mapping using the <i>P. bahamense</i> transcriptome and translated protein databases of <i>Marinovum algicola</i>, <i>Alexandrium</i> sp., <i>Cylindrospermopsis raciborskii</i>, and <i>Symbiodinium kawagutii</i> for annotation enabled the characterization of the proteins in <i>P. bahamense</i>. The highest number of annotated hits were obtained from <i>M. algicola</i> and highlighted the contribution of microorganisms associated with <i>P. bahamense</i>. Proteins involved in dimethylsulfoniopropionate (DMSP) degradation such as propionyl CoA synthethase and acryloyl-CoA reductase were identified, suggesting the DMSP cleavage pathway as the preferred route in this dinoflagellate. Most of the annotated proteins were involved in amino acid biosynthesis and carbohydrate degradation and metabolism, indicating the active roles of these molecules in the vegetative stage of <i>P. bahamense</i>. This characterization provides baseline information on the cellular machinery and the molecular basis of the ecophysiology of <i>P. bahamense</i>. |
| first_indexed | 2024-03-10T03:57:08Z |
| format | Article |
| id | doaj.art-fd99476636ee48099cde54837eb8196a |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:57:08Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fd99476636ee48099cde54837eb8196a2023-11-23T08:44:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241333210.3390/ijms222413332Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal ProcessesBryan John J. Subong0Zabrina Bernice L. Malto1Arturo O. Lluisma2Rhodora V. Azanza3Lilibeth A. Salvador-Reyes4Marine Science Institute, College of Science, University of the Philippines Diliman, P. Velasquez St., UP Diliman, Quezon City 1101, PhilippinesMarine Science Institute, College of Science, University of the Philippines Diliman, P. Velasquez St., UP Diliman, Quezon City 1101, PhilippinesMarine Science Institute, College of Science, University of the Philippines Diliman, P. Velasquez St., UP Diliman, Quezon City 1101, PhilippinesMarine Science Institute, College of Science, University of the Philippines Diliman, P. Velasquez St., UP Diliman, Quezon City 1101, PhilippinesMarine Science Institute, College of Science, University of the Philippines Diliman, P. Velasquez St., UP Diliman, Quezon City 1101, PhilippinesProteins, lipids, and carbohydrates from the harmful algal bloom (HAB)-causing organism <i>Pyrodinium bahamense</i> were characterized to obtain insights into the biochemical processes in this environmentally relevant dinoflagellate. Shotgun proteomics using label-free quantitation followed by proteome mapping using the <i>P. bahamense</i> transcriptome and translated protein databases of <i>Marinovum algicola</i>, <i>Alexandrium</i> sp., <i>Cylindrospermopsis raciborskii</i>, and <i>Symbiodinium kawagutii</i> for annotation enabled the characterization of the proteins in <i>P. bahamense</i>. The highest number of annotated hits were obtained from <i>M. algicola</i> and highlighted the contribution of microorganisms associated with <i>P. bahamense</i>. Proteins involved in dimethylsulfoniopropionate (DMSP) degradation such as propionyl CoA synthethase and acryloyl-CoA reductase were identified, suggesting the DMSP cleavage pathway as the preferred route in this dinoflagellate. Most of the annotated proteins were involved in amino acid biosynthesis and carbohydrate degradation and metabolism, indicating the active roles of these molecules in the vegetative stage of <i>P. bahamense</i>. This characterization provides baseline information on the cellular machinery and the molecular basis of the ecophysiology of <i>P. bahamense</i>.https://www.mdpi.com/1422-0067/22/24/13332<i>Pyrodinium bahamense</i>proteomicsbiochemical pathwaysharmful algal bloomparalytic shellfish toxinsbiomolecules |
| spellingShingle | Bryan John J. Subong Zabrina Bernice L. Malto Arturo O. Lluisma Rhodora V. Azanza Lilibeth A. Salvador-Reyes Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes International Journal of Molecular Sciences <i>Pyrodinium bahamense</i> proteomics biochemical pathways harmful algal bloom paralytic shellfish toxins biomolecules |
| title | Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes |
| title_full | Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes |
| title_fullStr | Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes |
| title_full_unstemmed | Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes |
| title_short | Biochemical Mapping of <i>Pyrodinium bahamense</i> Unveils Molecular Underpinnings behind Organismal Processes |
| title_sort | biochemical mapping of i pyrodinium bahamense i unveils molecular underpinnings behind organismal processes |
| topic | <i>Pyrodinium bahamense</i> proteomics biochemical pathways harmful algal bloom paralytic shellfish toxins biomolecules |
| url | https://www.mdpi.com/1422-0067/22/24/13332 |
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