The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture
<p>Bisphosphonates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of bisphosphonate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 10<sup>8</sup>...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Dentistry, Universitas Indonesia
2015-10-01
|
| Series: | Journal of Dentistry Indonesia |
| Online Access: | http://jdentistry.ui.ac.id/index.php/JDI/article/view/798 |
| _version_ | 1818140797490429952 |
|---|---|
| author | Sri Angky Soekanto Keiichi Ohya |
| author_facet | Sri Angky Soekanto Keiichi Ohya |
| author_sort | Sri Angky Soekanto |
| collection | DOAJ |
| description | <p>Bisphosphonates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of bisphosphonate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 10<sup>8</sup>M 1α, 25-dihydroxyvitamin D<sub>3 </sub>(1α, 25(OH)<sub>2</sub>D<sub>3</sub>) numerous clusters of mononuclear and multinucleated cells formed, which stained positive for tartrate-resistant acid phosphatase (TRAP-positive). 1α, 25(OH)<sub>2</sub>D<sub>3</sub> is known to stimulate osteoclast-like cell formation in a mouse bone marrow culture. Adding 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) inhibited the increased formation of osteoclast-like cells stimulated by this stimulant. A time-course experimental model showed that the number of osteoclast-like cells decreased slightly when drugs were given early in the culture period and decreased markedly when the drugs were given later or continuously in the culture period. These findings suggested that bisphosphonate had an effect on mature stage and significantly inhibit bone destruction by inhibit osteoclast-like cells formation. The amount of PGE<sub>2 </sub>production stimulated by 1 α, 25(OH)<sub>2</sub>D<sub>3 </sub>was dose depedently higher with HEBP and 3-amino-1-hydroxypropylidene-1, 1-bisphosphonate (APD). Showing that PGE<sub>2 </sub>production is high at the end of culture when the cells are going to undergo apoptosis. This showed in part, the known bone-resorbing activity of these agents.</p> |
| first_indexed | 2024-12-11T10:49:42Z |
| format | Article |
| id | doaj.art-fd9acb79deb045f3b79ff26d08fc458c |
| institution | Directory Open Access Journal |
| issn | 1693-9697 2355-4800 |
| language | English |
| last_indexed | 2024-12-11T10:49:42Z |
| publishDate | 2015-10-01 |
| publisher | Faculty of Dentistry, Universitas Indonesia |
| record_format | Article |
| series | Journal of Dentistry Indonesia |
| spelling | doaj.art-fd9acb79deb045f3b79ff26d08fc458c2022-12-22T01:10:21ZengFaculty of Dentistry, Universitas IndonesiaJournal of Dentistry Indonesia1693-96972355-48002015-10-016161110.14693/jdi.v6i1.798692The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow CultureSri Angky Soekanto0Keiichi Ohya1Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University<p>Bisphosphonates are reported to have an inhibitory effect on bone resorption in vivo and in vitro. The present study examined the effect of bisphosphonate on the formation of osteoclast-like cells in vitro. When mouse bone marrow cells were cultured for 8 days with 10<sup>8</sup>M 1α, 25-dihydroxyvitamin D<sub>3 </sub>(1α, 25(OH)<sub>2</sub>D<sub>3</sub>) numerous clusters of mononuclear and multinucleated cells formed, which stained positive for tartrate-resistant acid phosphatase (TRAP-positive). 1α, 25(OH)<sub>2</sub>D<sub>3</sub> is known to stimulate osteoclast-like cell formation in a mouse bone marrow culture. Adding 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) inhibited the increased formation of osteoclast-like cells stimulated by this stimulant. A time-course experimental model showed that the number of osteoclast-like cells decreased slightly when drugs were given early in the culture period and decreased markedly when the drugs were given later or continuously in the culture period. These findings suggested that bisphosphonate had an effect on mature stage and significantly inhibit bone destruction by inhibit osteoclast-like cells formation. The amount of PGE<sub>2 </sub>production stimulated by 1 α, 25(OH)<sub>2</sub>D<sub>3 </sub>was dose depedently higher with HEBP and 3-amino-1-hydroxypropylidene-1, 1-bisphosphonate (APD). Showing that PGE<sub>2 </sub>production is high at the end of culture when the cells are going to undergo apoptosis. This showed in part, the known bone-resorbing activity of these agents.</p>http://jdentistry.ui.ac.id/index.php/JDI/article/view/798 |
| spellingShingle | Sri Angky Soekanto Keiichi Ohya The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture Journal of Dentistry Indonesia |
| title | The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture |
| title_full | The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture |
| title_fullStr | The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture |
| title_full_unstemmed | The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture |
| title_short | The Effect of Bisphosphonate on The Osteoclast-Like Cell Formation in A Mouse Bone Marrow Culture |
| title_sort | effect of bisphosphonate on the osteoclast like cell formation in a mouse bone marrow culture |
| url | http://jdentistry.ui.ac.id/index.php/JDI/article/view/798 |
| work_keys_str_mv | AT sriangkysoekanto theeffectofbisphosphonateontheosteoclastlikecellformationinamousebonemarrowculture AT keiichiohya theeffectofbisphosphonateontheosteoclastlikecellformationinamousebonemarrowculture AT sriangkysoekanto effectofbisphosphonateontheosteoclastlikecellformationinamousebonemarrowculture AT keiichiohya effectofbisphosphonateontheosteoclastlikecellformationinamousebonemarrowculture |