Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease
Background: Brain damage is a severe and common pathology that leads to life-threatening diseases. Despite development in the research, the medical evidence of the effectiveness of potential neuroprotective medicines is insufficient. As a result, there is an immense and urgent demand for promising m...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | IBRO Neuroscience Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667242122000975 |
| _version_ | 1797804569293488128 |
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| author | Mabozou Kpemissi Yendube T. Kantati Veeresh Prabhakar Veerapur Kwashie Eklu-Gadegbeku Zurina Hassan |
| author_facet | Mabozou Kpemissi Yendube T. Kantati Veeresh Prabhakar Veerapur Kwashie Eklu-Gadegbeku Zurina Hassan |
| author_sort | Mabozou Kpemissi |
| collection | DOAJ |
| description | Background: Brain damage is a severe and common pathology that leads to life-threatening diseases. Despite development in the research, the medical evidence of the effectiveness of potential neuroprotective medicines is insufficient. As a result, there is an immense and urgent demand for promising medication. For millennia, herbal remedies were a fundamental aspect of medical treatments. Combretum micranthum (CM), a plant of the family Combretaceae in sub-Saharan Africa, has been utilized in folklore medicine to cure diverse human ailments. In order to develop a neuroprotective phytomedicine, the current research was undertaken to explore the antioxidant, anti-inflammatory, anticholinesterase and neuroprotective potential of CM extract. Methods: Colorimetric methods were used to determine CM antioxidant activity, in-vitro protein denaturation and membrane destabilization assays were used to evaluate its anti-inflammatory capacity, anticholinesterase activity was carried out using Ellman’s method, and neuroprotective potential was assessed on brain homogenate stressed with ferric chloride and ascorbic acid (FeCl2-AA) by assessing the lipoperoxidation biomarker malondialdehyde (MDA). Results: In Ferric Reducing Antioxidant Power (IC50 = 27.15 ± 0.06 µg/mL) and Total Antioxidant Capacity (IC50 = 31.13 ± 0.02 µg/mL), CM extract demonstrated strong antioxidant activity. Anti-inflammatory effect were improved in heat-induced Egg albumin and BSA denaturation (IC 50 = 46.35 ± 1.53 and 23.94 ± 1.10 µg/mL) as well as heat and hypotonia induced membrane destabilization (IC 50 = 20.96 ± 0.11 and 16.75 ± 0.94 µg/mL).CM extract showed strong anticholinesterase activity (IC 50 = 59.85 ± 0.91 µg/mL). In an ex-vivo neuroprotective model, CM extract showed substantial inhibition (p < 0.001) of oxidative damage caused by FeCl2-AA in brain tissue. Conclusion: C. micranthum may be a good candidate for its probable neuroprotective potential. Its neuroprotective benefits might be attributed to its antioxidant, anti-inflammatory and anticholinesterase effects. |
| first_indexed | 2024-03-13T05:39:09Z |
| format | Article |
| id | doaj.art-fd9cd2fdcdfd403bb02f5991441d1aff |
| institution | Directory Open Access Journal |
| issn | 2667-2421 |
| language | English |
| last_indexed | 2024-03-13T05:39:09Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IBRO Neuroscience Reports |
| spelling | doaj.art-fd9cd2fdcdfd403bb02f5991441d1aff2023-06-14T04:34:35ZengElsevierIBRO Neuroscience Reports2667-24212023-06-01142127Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative diseaseMabozou Kpemissi0Yendube T. Kantati1Veeresh Prabhakar Veerapur2Kwashie Eklu-Gadegbeku3Zurina Hassan4Centre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; University of Lomé, Togo; Sree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, India; Correspondence to: Major in Pharmacology and Physiology Faculty of Sciences, University of Lomé, Togo.University of Lomé, TogoSree Siddaganga College of Pharmacy, B.H. Road, Tumkur 572 102, Karnataka, IndiaUniversity of Lomé, TogoCentre for Drug Research, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; Corresponding author.Background: Brain damage is a severe and common pathology that leads to life-threatening diseases. Despite development in the research, the medical evidence of the effectiveness of potential neuroprotective medicines is insufficient. As a result, there is an immense and urgent demand for promising medication. For millennia, herbal remedies were a fundamental aspect of medical treatments. Combretum micranthum (CM), a plant of the family Combretaceae in sub-Saharan Africa, has been utilized in folklore medicine to cure diverse human ailments. In order to develop a neuroprotective phytomedicine, the current research was undertaken to explore the antioxidant, anti-inflammatory, anticholinesterase and neuroprotective potential of CM extract. Methods: Colorimetric methods were used to determine CM antioxidant activity, in-vitro protein denaturation and membrane destabilization assays were used to evaluate its anti-inflammatory capacity, anticholinesterase activity was carried out using Ellman’s method, and neuroprotective potential was assessed on brain homogenate stressed with ferric chloride and ascorbic acid (FeCl2-AA) by assessing the lipoperoxidation biomarker malondialdehyde (MDA). Results: In Ferric Reducing Antioxidant Power (IC50 = 27.15 ± 0.06 µg/mL) and Total Antioxidant Capacity (IC50 = 31.13 ± 0.02 µg/mL), CM extract demonstrated strong antioxidant activity. Anti-inflammatory effect were improved in heat-induced Egg albumin and BSA denaturation (IC 50 = 46.35 ± 1.53 and 23.94 ± 1.10 µg/mL) as well as heat and hypotonia induced membrane destabilization (IC 50 = 20.96 ± 0.11 and 16.75 ± 0.94 µg/mL).CM extract showed strong anticholinesterase activity (IC 50 = 59.85 ± 0.91 µg/mL). In an ex-vivo neuroprotective model, CM extract showed substantial inhibition (p < 0.001) of oxidative damage caused by FeCl2-AA in brain tissue. Conclusion: C. micranthum may be a good candidate for its probable neuroprotective potential. Its neuroprotective benefits might be attributed to its antioxidant, anti-inflammatory and anticholinesterase effects.http://www.sciencedirect.com/science/article/pii/S2667242122000975Combretum micranthumAnti-inflammatoryAntioxidantAnticholinesteraseNeuroprotection |
| spellingShingle | Mabozou Kpemissi Yendube T. Kantati Veeresh Prabhakar Veerapur Kwashie Eklu-Gadegbeku Zurina Hassan Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease IBRO Neuroscience Reports Combretum micranthum Anti-inflammatory Antioxidant Anticholinesterase Neuroprotection |
| title | Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease |
| title_full | Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease |
| title_fullStr | Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease |
| title_full_unstemmed | Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease |
| title_short | Anti-cholinesterase, anti-inflammatory and antioxidant properties of Combretum micranthum G. Don: Potential implications in neurodegenerative disease |
| title_sort | anti cholinesterase anti inflammatory and antioxidant properties of combretum micranthum g don potential implications in neurodegenerative disease |
| topic | Combretum micranthum Anti-inflammatory Antioxidant Anticholinesterase Neuroprotection |
| url | http://www.sciencedirect.com/science/article/pii/S2667242122000975 |
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