Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro
Objective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applicationsdue to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select ascaffold that can support, induce, and restore biological funct...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2024-02-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://www.celljournal.org/article_710207_142ed895f273876a0a00ef5eafd0b69a.pdf |
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| author | Mehrdad Zahiri-Toosi Seyed Jalal Zargar Ehsan Seyedjafari Mostafa Saberian Marziehsadat Ahmadi |
| author_facet | Mehrdad Zahiri-Toosi Seyed Jalal Zargar Ehsan Seyedjafari Mostafa Saberian Marziehsadat Ahmadi |
| author_sort | Mehrdad Zahiri-Toosi |
| collection | DOAJ |
| description | Objective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applicationsdue to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select ascaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSCsurvival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrousscaffolds apt for applications in bone tissue engineering.Materials and Methods: In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid(PLLA) polymer. The PLLA fibers’ surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles.Results: The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffoldpositively influenced the attachment, growth, and osteogenic differentiation of MSCs.Conclusion: Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenicdifferentiation of MSCs on electrospun PLLA scaffolds. |
| first_indexed | 2024-04-25T01:25:19Z |
| format | Article |
| id | doaj.art-fd9d03f5ab934f88bfe33d3da36df78d |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-25T01:25:19Z |
| publishDate | 2024-02-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-fd9d03f5ab934f88bfe33d3da36df78d2024-03-09T07:20:35ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142024-02-0126213013810.22074/cellj.2024.2008921.1388710207Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In VitroMehrdad Zahiri-Toosi0Seyed Jalal Zargar1Ehsan Seyedjafari2Mostafa Saberian3Marziehsadat Ahmadi4Department of Cell and Molecular Biology, International Campus-Kish, University of Tehran, Kish Island, IranDepartment of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, IranSchool of Biotechnology, College of Science, University of Tehran, Tehran, IranDepartment of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranDepartment of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, IranObjective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applicationsdue to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select ascaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSCsurvival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrousscaffolds apt for applications in bone tissue engineering.Materials and Methods: In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid(PLLA) polymer. The PLLA fibers’ surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles.Results: The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffoldpositively influenced the attachment, growth, and osteogenic differentiation of MSCs.Conclusion: Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenicdifferentiation of MSCs on electrospun PLLA scaffolds.https://www.celljournal.org/article_710207_142ed895f273876a0a00ef5eafd0b69a.pdfcollagennano-hydroxyapatitetissue engineering |
| spellingShingle | Mehrdad Zahiri-Toosi Seyed Jalal Zargar Ehsan Seyedjafari Mostafa Saberian Marziehsadat Ahmadi Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro Cell Journal collagen nano-hydroxyapatite tissue engineering |
| title | Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro |
| title_full | Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro |
| title_fullStr | Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro |
| title_full_unstemmed | Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro |
| title_short | Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro |
| title_sort | simultaneous coating of electrospun nanofibers with bioactive molecules for stem cell osteogenesis in vitro |
| topic | collagen nano-hydroxyapatite tissue engineering |
| url | https://www.celljournal.org/article_710207_142ed895f273876a0a00ef5eafd0b69a.pdf |
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