Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers
The purpose of this study was to evaluate the relationship between human papillomavirus (HPV16/18), Epstein–Barr virus (EBV), and human cytomegalovirus (HCMV) infections and the occurrence of ovarian cancer in 48 women, of whom 36 underwent surgery and chemotherapy (group A), 12 in whom surgery was...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/3/397 |
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| author | Beniamin Oskar Grabarek Piotr Ossowski Justyna Czarniecka Mateusz Ożóg Justyna Prucnal Ireneusz Dziuba Aleksander Ostenda Konrad Dziobek Dariusz Boroń Wojciech Peszek Piotr Kras Szymon Januszyk Maciej Dąbala Tomasz Kasela Marcin Opławski |
| author_facet | Beniamin Oskar Grabarek Piotr Ossowski Justyna Czarniecka Mateusz Ożóg Justyna Prucnal Ireneusz Dziuba Aleksander Ostenda Konrad Dziobek Dariusz Boroń Wojciech Peszek Piotr Kras Szymon Januszyk Maciej Dąbala Tomasz Kasela Marcin Opławski |
| author_sort | Beniamin Oskar Grabarek |
| collection | DOAJ |
| description | The purpose of this study was to evaluate the relationship between human papillomavirus (HPV16/18), Epstein–Barr virus (EBV), and human cytomegalovirus (HCMV) infections and the occurrence of ovarian cancer in 48 women, of whom 36 underwent surgery and chemotherapy (group A), 12 in whom surgery was sufficient (group B), and 60 with endometroid endometrial cancer stage G1-G3 (group C), compared to patients in whom the uterus and its appendages were removed for nononcological reasons (control group). The detection of HPV, EBV, and HCMV in tumor tissue and normal tissue was performed using the real-time polymerase chain reaction (RT-PCR) technique. A statistically significantly higher risk of endometrial cancer was noted in patients infected only with HCMV (OR > 1; <i>p</i> < 0.05). In contrast, a significantly higher risk of ovarian cancer in group A was associated with HPV16, HPV18, and EBV (OR > 1; <i>p</i> < 0.05); a significantly higher risk of ovarian cancer in group B was associated with HPV18 and HMCV (OR > 1; <i>p</i> < 0.05). The obtained results suggest that HCMV infection is associated with the development of a stage of ovarian cancer when treatment can be completed with surgery alone. Meanwhile, EBV appears to be responsible for the development of ovarian cancer in more advanced stages. |
| first_indexed | 2024-03-11T06:03:47Z |
| format | Article |
| id | doaj.art-fd9ed29d88984c5abf620c335f45b5cc |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T06:03:47Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fd9ed29d88984c5abf620c335f45b5cc2023-11-17T13:09:24ZengMDPI AGPathogens2076-08172023-03-0112339710.3390/pathogens12030397Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian CancersBeniamin Oskar Grabarek0Piotr Ossowski1Justyna Czarniecka2Mateusz Ożóg3Justyna Prucnal4Ireneusz Dziuba5Aleksander Ostenda6Konrad Dziobek7Dariusz Boroń8Wojciech Peszek9Piotr Kras10Szymon Januszyk11Maciej Dąbala12Tomasz Kasela13Marcin Opławski14Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Microbiology, SP ZOZ Szpital Wielospecjalistyczny w Jaworznie, 43-600 Jaworzno, PolandDepartment of Pathology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandFaculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandThe purpose of this study was to evaluate the relationship between human papillomavirus (HPV16/18), Epstein–Barr virus (EBV), and human cytomegalovirus (HCMV) infections and the occurrence of ovarian cancer in 48 women, of whom 36 underwent surgery and chemotherapy (group A), 12 in whom surgery was sufficient (group B), and 60 with endometroid endometrial cancer stage G1-G3 (group C), compared to patients in whom the uterus and its appendages were removed for nononcological reasons (control group). The detection of HPV, EBV, and HCMV in tumor tissue and normal tissue was performed using the real-time polymerase chain reaction (RT-PCR) technique. A statistically significantly higher risk of endometrial cancer was noted in patients infected only with HCMV (OR > 1; <i>p</i> < 0.05). In contrast, a significantly higher risk of ovarian cancer in group A was associated with HPV16, HPV18, and EBV (OR > 1; <i>p</i> < 0.05); a significantly higher risk of ovarian cancer in group B was associated with HPV18 and HMCV (OR > 1; <i>p</i> < 0.05). The obtained results suggest that HCMV infection is associated with the development of a stage of ovarian cancer when treatment can be completed with surgery alone. Meanwhile, EBV appears to be responsible for the development of ovarian cancer in more advanced stages.https://www.mdpi.com/2076-0817/12/3/397human papillomavirusEpstein–Barr virushuman cytomegalovirusovarian cancerendometrioid endometrial cancer |
| spellingShingle | Beniamin Oskar Grabarek Piotr Ossowski Justyna Czarniecka Mateusz Ożóg Justyna Prucnal Ireneusz Dziuba Aleksander Ostenda Konrad Dziobek Dariusz Boroń Wojciech Peszek Piotr Kras Szymon Januszyk Maciej Dąbala Tomasz Kasela Marcin Opławski Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers Pathogens human papillomavirus Epstein–Barr virus human cytomegalovirus ovarian cancer endometrioid endometrial cancer |
| title | Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers |
| title_full | Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers |
| title_fullStr | Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers |
| title_full_unstemmed | Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers |
| title_short | Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers |
| title_sort | detection and genotyping of human papillomavirus hpv16 18 epstein barr virus ebv and human cytomegalovirus hcmv in endometrial endometroid and ovarian cancers |
| topic | human papillomavirus Epstein–Barr virus human cytomegalovirus ovarian cancer endometrioid endometrial cancer |
| url | https://www.mdpi.com/2076-0817/12/3/397 |
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