Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines
We investigated the anticancer potential of 5-amino-2-[p-bromophenyl]-benzoxazole [BB] which is a new benzoxazole derivative in different breast cancer cell lines. BB was applied to estrogen receptor positive [ER+] MCF-7 and receptor negative [ER-] MDA-MB cell lines and its effects were determined b...
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| Format: | Article |
| Language: | English |
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Society of Turaz Bilim
2022-06-01
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| Series: | Medicine Science |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=132047 |
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| author | Funda Kosova Ozlem Temiz Arpaci Ibrahim Tuglu Ercument Olmez Feyzan Ozdal Kurt Gorkem Kismali Zeki Ari Mustafa Arisoy |
| author_facet | Funda Kosova Ozlem Temiz Arpaci Ibrahim Tuglu Ercument Olmez Feyzan Ozdal Kurt Gorkem Kismali Zeki Ari Mustafa Arisoy |
| author_sort | Funda Kosova |
| collection | DOAJ |
| description | We investigated the anticancer potential of 5-amino-2-[p-bromophenyl]-benzoxazole [BB] which is a new benzoxazole derivative in different breast cancer cell lines. BB was applied to estrogen receptor positive [ER+] MCF-7 and receptor negative [ER-] MDA-MB cell lines and its effects were determined by histopathological examinations, viability test [MTT], immunocytochemistry assays [Tunnel, VEGF and eNOS]. Moreover, its effects on apoptozis-related proteins such as Apaf-1, cytochrome C, caspase-3, bcl-2 and NF-κB were examined by western blot. Histopathological examinations showed that BB killed many cells for both cancer cell line while the cells lost their adhesion in MCF-7 and lost their adhesion and epiteloid morphology in MDA-MB. MTT analyses demonstrated that BB caused a clear dose depended toxic effect for both cell types. BB application resulted in an increase labeled apoptotic cells after Tunnel staining which was more obvious for MDA-MB compared to that of MCF-7. VEGF staining was decreased after BB application in both cell lines. The decrease of VEGF staining was clearer for MDA-MB compared to that of MCF-7. The status of oxidative stress was shown by eNOS immunocytochemistry which was increase after BB application in both cell lines. The levels of Apaf-1 and bcl-2 were found to be similar while caspase 9 and NF-κB levels were decreased compared to the control group after BB application for both cell lines. On the other hand, cytochrome C levels were slightly increased in MCF-7 cells while this increase was found very obvious in MDA-MB cell lines in BB groups. In conclusion, BB which is a new benzoxazole derivative have shown significant anticancer effects by increasing apoptosis and decreasing angiogenesis in MCF-7 or MDA-MB breast cancer cell lines. These effects of BB seem to be more prominent for [ER-] MDA-MB cell lines which mimic more aggressive type of breast cancer. These results suggest that BB may be useful to treat [ER-] breast cancers and may be added to treatment protocols. [Med-Science 2022; 11(2.000): 825-30] |
| first_indexed | 2024-03-08T06:53:34Z |
| format | Article |
| id | doaj.art-fda1b39683c84a23bb31167e52bd5e79 |
| institution | Directory Open Access Journal |
| issn | 2147-0634 |
| language | English |
| last_indexed | 2024-03-08T06:53:34Z |
| publishDate | 2022-06-01 |
| publisher | Society of Turaz Bilim |
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| series | Medicine Science |
| spelling | doaj.art-fda1b39683c84a23bb31167e52bd5e792024-02-03T06:40:28ZengSociety of Turaz BilimMedicine Science2147-06342022-06-011128253010.5455/medscience.2021.10.337132047Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell linesFunda Kosova0Ozlem Temiz Arpaci1Ibrahim Tuglu2Ercument Olmez3Feyzan Ozdal Kurt4Gorkem Kismali5Zeki Ari6Mustafa Arisoy7Celal Bayar Unv Health Campus Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06100, Tandogan, Ankara, Turkey Department of Histology and Embryology of Celal Bayar University Medical Faculty, Manisa, Turkey Department of Pharmacology of Celal Bayar University Medical Faculty, Manisa, Turkey Department of Biology of Celal Bayar University Science and Art Faculty, Manisa, Turkey Department of Biochemistry of Ankara University Veterinary Faculty, Ankara, Turkey Department of Medical Biochemistry of Celal Bayar University Medical Faculty, Manisa, Turkey Drogsan Pharmaceuticals, RD Department, Gazi Universitesi Golbasi Yerleskesi, Teknoplaza Binasi, B Blok BB10, 06830, Golbasi Ankara, TurkeyWe investigated the anticancer potential of 5-amino-2-[p-bromophenyl]-benzoxazole [BB] which is a new benzoxazole derivative in different breast cancer cell lines. BB was applied to estrogen receptor positive [ER+] MCF-7 and receptor negative [ER-] MDA-MB cell lines and its effects were determined by histopathological examinations, viability test [MTT], immunocytochemistry assays [Tunnel, VEGF and eNOS]. Moreover, its effects on apoptozis-related proteins such as Apaf-1, cytochrome C, caspase-3, bcl-2 and NF-κB were examined by western blot. Histopathological examinations showed that BB killed many cells for both cancer cell line while the cells lost their adhesion in MCF-7 and lost their adhesion and epiteloid morphology in MDA-MB. MTT analyses demonstrated that BB caused a clear dose depended toxic effect for both cell types. BB application resulted in an increase labeled apoptotic cells after Tunnel staining which was more obvious for MDA-MB compared to that of MCF-7. VEGF staining was decreased after BB application in both cell lines. The decrease of VEGF staining was clearer for MDA-MB compared to that of MCF-7. The status of oxidative stress was shown by eNOS immunocytochemistry which was increase after BB application in both cell lines. The levels of Apaf-1 and bcl-2 were found to be similar while caspase 9 and NF-κB levels were decreased compared to the control group after BB application for both cell lines. On the other hand, cytochrome C levels were slightly increased in MCF-7 cells while this increase was found very obvious in MDA-MB cell lines in BB groups. In conclusion, BB which is a new benzoxazole derivative have shown significant anticancer effects by increasing apoptosis and decreasing angiogenesis in MCF-7 or MDA-MB breast cancer cell lines. These effects of BB seem to be more prominent for [ER-] MDA-MB cell lines which mimic more aggressive type of breast cancer. These results suggest that BB may be useful to treat [ER-] breast cancers and may be added to treatment protocols. [Med-Science 2022; 11(2.000): 825-30]http://www.ejmanager.com/fulltextpdf.php?mno=132047benzoxazole derivativebreast cancerapoptosisangiogenesis |
| spellingShingle | Funda Kosova Ozlem Temiz Arpaci Ibrahim Tuglu Ercument Olmez Feyzan Ozdal Kurt Gorkem Kismali Zeki Ari Mustafa Arisoy Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines Medicine Science benzoxazole derivative breast cancer apoptosis angiogenesis |
| title | Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines |
| title_full | Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines |
| title_fullStr | Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines |
| title_full_unstemmed | Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines |
| title_short | Anticancer effects of a newly-synthesized benzoxazole-derived 5-amino-2- [P-bromophenyl]-benzoxazole in breast cancer cell lines |
| title_sort | anticancer effects of a newly synthesized benzoxazole derived 5 amino 2 p bromophenyl benzoxazole in breast cancer cell lines |
| topic | benzoxazole derivative breast cancer apoptosis angiogenesis |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=132047 |
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