Stimulation of erythroblast maturation in vitro by sphingolipids
A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography–mass spectrometry. The biol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1974-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520367560 |
| _version_ | 1819072309752758272 |
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| author | R.B. Clayton J.M. Cooper Tore Curstedt Jan Sjövall Henry Borsook J. Chin A. Schwarz |
| author_facet | R.B. Clayton J.M. Cooper Tore Curstedt Jan Sjövall Henry Borsook J. Chin A. Schwarz |
| author_sort | R.B. Clayton |
| collection | DOAJ |
| description | A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography–mass spectrometry. The biologically active components are sphingosine ceramides of tetracosanoic and dehydrotetracosanoic acids. Tests of a series of related ceramides show a high degree of structural specificity for the C24-N-acyl compounds with significant but markedly lower activity of the C22 analog. Commercially available sphingomyelin shows activity comparable to that of the tetracosanoic acid ceramide. Sphingosine and tetracosanic acid supplied in equimolar amounts have negligible activity. The results, in the context of other findings, suggest a possible supportive role of plasma ceramides and sphingomyelins in red cell maturation. |
| first_indexed | 2024-12-21T17:35:41Z |
| format | Article |
| id | doaj.art-fda597254e5f4f95b4d9f8d9a1010cc0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T17:35:41Z |
| publishDate | 1974-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fda597254e5f4f95b4d9f8d9a1010cc02022-12-21T18:55:47ZengElsevierJournal of Lipid Research0022-22751974-11-01156557562Stimulation of erythroblast maturation in vitro by sphingolipidsR.B. Clayton0J.M. Cooper1Tore Curstedt2Jan Sjövall3Henry Borsook4J. Chin5A. Schwarz6Department of Psychiatry, Stanford Medical Center, Stanford California 94305Department of Psychiatry, Stanford Medical Center, Stanford California 94305Kemiska Institutionen, Karolinska Institutet, S-10401 Stockholm, Sweden;Kemiska Institutionen, Karolinska Institutet, S-10401 Stockholm, Sweden;University of California, Space Sciences Laboratory, Berkeley, California 94720University of California, Space Sciences Laboratory, Berkeley, California 94720University of California, Space Sciences Laboratory, Berkeley, California 94720A lipid factor previously isolated from leukocytes and found to stimulate basophilic erythroblast formation in an in vitro system of incubated rabbit bone marrow cells has been analyzed by thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography–mass spectrometry. The biologically active components are sphingosine ceramides of tetracosanoic and dehydrotetracosanoic acids. Tests of a series of related ceramides show a high degree of structural specificity for the C24-N-acyl compounds with significant but markedly lower activity of the C22 analog. Commercially available sphingomyelin shows activity comparable to that of the tetracosanoic acid ceramide. Sphingosine and tetracosanic acid supplied in equimolar amounts have negligible activity. The results, in the context of other findings, suggest a possible supportive role of plasma ceramides and sphingomyelins in red cell maturation.http://www.sciencedirect.com/science/article/pii/S0022227520367560erythrocyte maturation in vitrobasophilic erythroblaststetracosanoic (lignoceric) acid ceramidessphingomyelinerythropoiesis |
| spellingShingle | R.B. Clayton J.M. Cooper Tore Curstedt Jan Sjövall Henry Borsook J. Chin A. Schwarz Stimulation of erythroblast maturation in vitro by sphingolipids Journal of Lipid Research erythrocyte maturation in vitro basophilic erythroblasts tetracosanoic (lignoceric) acid ceramides sphingomyelin erythropoiesis |
| title | Stimulation of erythroblast maturation in vitro by sphingolipids |
| title_full | Stimulation of erythroblast maturation in vitro by sphingolipids |
| title_fullStr | Stimulation of erythroblast maturation in vitro by sphingolipids |
| title_full_unstemmed | Stimulation of erythroblast maturation in vitro by sphingolipids |
| title_short | Stimulation of erythroblast maturation in vitro by sphingolipids |
| title_sort | stimulation of erythroblast maturation in vitro by sphingolipids |
| topic | erythrocyte maturation in vitro basophilic erythroblasts tetracosanoic (lignoceric) acid ceramides sphingomyelin erythropoiesis |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520367560 |
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