Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid
Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/12/9/854 |
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| author | Hai Doan Do Christine Ménager Aude Michel Johanne Seguin Tawba Korichi Hélène Dhotel Corinne Marie Bich-Thuy Doan Nathalie Mignet |
| author_facet | Hai Doan Do Christine Ménager Aude Michel Johanne Seguin Tawba Korichi Hélène Dhotel Corinne Marie Bich-Thuy Doan Nathalie Mignet |
| author_sort | Hai Doan Do |
| collection | DOAJ |
| description | Cationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities <i>r<sub>2</sub></i> and <i>r<sub>1</sub></i> was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy. |
| first_indexed | 2024-03-10T16:29:16Z |
| format | Article |
| id | doaj.art-fda67c673f2a454fa3a9bc7f066c04e3 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T16:29:16Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-fda67c673f2a454fa3a9bc7f066c04e32023-11-20T13:01:10ZengMDPI AGPharmaceutics1999-49232020-09-0112985410.3390/pharmaceutics12090854Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic AcidHai Doan Do0Christine Ménager1Aude Michel2Johanne Seguin3Tawba Korichi4Hélène Dhotel5Corinne Marie6Bich-Thuy Doan7Nathalie Mignet8Université de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceSorbonne Université, Laboratoire PHENIX (Physicochimie des Electrolytes et Nanosystèmes Interfaciaux), CNRS, 4 Place Jussieu, F-75005 Paris, FranceSorbonne Université, Laboratoire PHENIX (Physicochimie des Electrolytes et Nanosystèmes Interfaciaux), CNRS, 4 Place Jussieu, F-75005 Paris, FranceUniversité de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceUniversité de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceUniversité de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceUniversité de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceChimie ParisTech PSL Research University, Institute of Chemistry for Life and Health Sciences, i-CleHS, SEISAD, F-75005 Paris, FranceUniversité de Paris, UTCBS (Chemical and Biological Technologies for Health Unit), CNRS, INSERM, 75006 Paris, FranceCationic liposomes have been considered as potential vectors for gene delivery thanks to their ability to transfect cells with high efficiency. Recently, the combination of diagnostic agent and therapeutic agents in the same particle to form a theranostic system has been reported. Magnetic liposomes are one of these examples. Due to the magnetic nanoparticles encapsulated in the liposomes, they can act as a drug delivery system and, at the same time, a magnetic resonance imaging contrast enhancement agent or hyperthermia. In this work, nucleic acid delivery systems based on magnetic cationic liposomes (MCLs) were developed. Two different techniques, reverse phase evaporation and cosolvent sonication, were employed for liposome preparation. Both strategies produced MCLs of less than 200 nm with highly positive charge. Enhancement of their transverse and longitudinal relaxivities <i>r<sub>2</sub></i> and <i>r<sub>1</sub></i> was obtained with both kinds of magnetic liposomes compared to free magnetic nanoparticles. Moreover, these MCLs showed high capacity to form complexes and transfect CT-26 cells using the antibiotic-free pFAR4-luc plasmid. The transfection enhancement with magnetofection was also carried out in CT26 cells. These results suggested that our MCLs could be a promising candidate for image-guided gene therapy.https://www.mdpi.com/1999-4923/12/9/854magnetic cationic liposomenucleic acid deliverymagnetofectionMRImagnetic targeting |
| spellingShingle | Hai Doan Do Christine Ménager Aude Michel Johanne Seguin Tawba Korichi Hélène Dhotel Corinne Marie Bich-Thuy Doan Nathalie Mignet Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid Pharmaceutics magnetic cationic liposome nucleic acid delivery magnetofection MRI magnetic targeting |
| title | Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid |
| title_full | Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid |
| title_fullStr | Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid |
| title_full_unstemmed | Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid |
| title_short | Development of Theranostic Cationic Liposomes Designed for Image-Guided Delivery of Nucleic Acid |
| title_sort | development of theranostic cationic liposomes designed for image guided delivery of nucleic acid |
| topic | magnetic cationic liposome nucleic acid delivery magnetofection MRI magnetic targeting |
| url | https://www.mdpi.com/1999-4923/12/9/854 |
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