The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice
Abstract Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen ma...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45986-4 |
| _version_ | 1797630347631919104 |
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| author | Narges Jamshidian-Ghalehsefidi Farzaneh Rabiee Marziyeh Tavalaee Shaghayegh Kiani Farnaz Pouriayevali Mazdak Razi Maurizio Dattilo Mohammad Hossein Nasr-Esfahani |
| author_facet | Narges Jamshidian-Ghalehsefidi Farzaneh Rabiee Marziyeh Tavalaee Shaghayegh Kiani Farnaz Pouriayevali Mazdak Razi Maurizio Dattilo Mohammad Hossein Nasr-Esfahani |
| author_sort | Narges Jamshidian-Ghalehsefidi |
| collection | DOAJ |
| description | Abstract Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen male C57 mice were addressed to a control (standard diet) or vitamin D deficient (VDD) diet for 14 weeks. The results show that compared to the standard diet, VDD increased final body weight and reduced sperm quality, caused damage to the testicular structure, and decreased the serum levels of testosterone. In addition, serum concentrations of homocysteine, vitamin B12, and sperm oxidative stress markers increased. In testicular tissues, the CBS and CSE protein levels were down-regulated whereas HO-1 was up-regulated at both mRNA and protein expression levels. Within a mice deprivation model, VDD deeply suppressed testosterone and impaired spermatogenesis with oxidative stress-mediated mechanisms. The effects of the deprivation appeared to be at least in part independent of genomic and receptor-mediated vitamin D actions and suggest a specific impairment of the alternative transsulfuration pathway. |
| first_indexed | 2024-03-11T11:07:04Z |
| format | Article |
| id | doaj.art-fdab05916c10475f9c512497557456b0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T11:07:04Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fdab05916c10475f9c512497557456b02023-11-12T12:12:28ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-45986-4The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient miceNarges Jamshidian-Ghalehsefidi0Farzaneh Rabiee1Marziyeh Tavalaee2Shaghayegh Kiani3Farnaz Pouriayevali4Mazdak Razi5Maurizio Dattilo6Mohammad Hossein Nasr-Esfahani7ACECR Institute of Higher Education, Isfahan BranchDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECRACECR Institute of Higher Education, Isfahan BranchDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECRDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECRDivision of Histology and Embryology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia UniversityR&D Department, ParthenogenACECR Institute of Higher Education, Isfahan BranchAbstract Vitamin D deficiency is a global health problem and has been linked to defective spermatogenesis and male infertility. In this study, we aimed to investigate the main enzymes involved in the transsulfuration pathway of 1-carbon metabolism, and spermatogenesis function. Therefore, sixteen male C57 mice were addressed to a control (standard diet) or vitamin D deficient (VDD) diet for 14 weeks. The results show that compared to the standard diet, VDD increased final body weight and reduced sperm quality, caused damage to the testicular structure, and decreased the serum levels of testosterone. In addition, serum concentrations of homocysteine, vitamin B12, and sperm oxidative stress markers increased. In testicular tissues, the CBS and CSE protein levels were down-regulated whereas HO-1 was up-regulated at both mRNA and protein expression levels. Within a mice deprivation model, VDD deeply suppressed testosterone and impaired spermatogenesis with oxidative stress-mediated mechanisms. The effects of the deprivation appeared to be at least in part independent of genomic and receptor-mediated vitamin D actions and suggest a specific impairment of the alternative transsulfuration pathway.https://doi.org/10.1038/s41598-023-45986-4 |
| spellingShingle | Narges Jamshidian-Ghalehsefidi Farzaneh Rabiee Marziyeh Tavalaee Shaghayegh Kiani Farnaz Pouriayevali Mazdak Razi Maurizio Dattilo Mohammad Hossein Nasr-Esfahani The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice Scientific Reports |
| title | The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice |
| title_full | The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice |
| title_fullStr | The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice |
| title_full_unstemmed | The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice |
| title_short | The role of the transsulfuration pathway in spermatogenesis of vitamin D deficient mice |
| title_sort | role of the transsulfuration pathway in spermatogenesis of vitamin d deficient mice |
| url | https://doi.org/10.1038/s41598-023-45986-4 |
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