Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that constitute a broad layer of gene regulation at both transcriptional and post-transcriptional levels from prokaryotes to eukaryotes. In embryonic development, they regulate the complex gene expression associated with the complexity of embry...
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00878/full |
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| author | Yan Wang Yan Wang Baojie Wang Xuqing Shao Mei Liu Keyong Jiang Mengqiang Wang Mengqiang Wang Lei Wang Lei Wang Lei Wang |
| author_facet | Yan Wang Yan Wang Baojie Wang Xuqing Shao Mei Liu Keyong Jiang Mengqiang Wang Mengqiang Wang Lei Wang Lei Wang Lei Wang |
| author_sort | Yan Wang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are endogenous small non-coding RNAs that constitute a broad layer of gene regulation at both transcriptional and post-transcriptional levels from prokaryotes to eukaryotes. In embryonic development, they regulate the complex gene expression associated with the complexity of embryogenesis. There is little information about miRNAs in the red claw crayfish (Cherax quadricarinatus), an important commercial species and a potential biological model. In the present study, miRNAs and their target genes were identified during three embryonic developmental stages of C. quadricarinatus. Nineteen known miRNAs and 331 novel ones belonging to 50 miRNA families were obtained. A total of 113 differentially expressed miRNAs were identified, and 2,575 target genes were predicted, of which 1,257 were annotated. Additionally, 63 target genes of 9 miRNAs in C. quadricarinatus were found to be related to embryonic development. For example, miR-10 and its target genes may regulate the nervous system development and body segmentation and miR-2788 may regulate cell proliferation to impact embryonic development. Moreover, miR-28 (target gene tutl), miR-50 (target gene fbx5), and miR-1260b (target gene sif) may co-regulate eye development of embryonic C. quadricarinatus. These miRNAs together with their target genes constitute a network for regulating the development of tissues and organs in the embryo of C. quadricarinatus. Our results lay a foundation for further study on the fundamental molecular and developmental mechanism of crustacean embryogenesis. |
| first_indexed | 2024-12-12T04:42:46Z |
| format | Article |
| id | doaj.art-fdab58b255f64e308e968ba322600265 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T04:42:46Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-fdab58b255f64e308e968ba3226002652022-12-22T00:37:44ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-09-011110.3389/fphys.2020.00878519930Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatusYan Wang0Yan Wang1Baojie Wang2Xuqing Shao3Mei Liu4Keyong Jiang5Mengqiang Wang6Mengqiang Wang7Lei Wang8Lei Wang9Lei Wang10CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaShandong Cigna Detection Technology Co., Ltd., Qingdao, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao, ChinaNational Laboratory for Marine Science and Technology, Center for Marine Molecular Biotechnology, Qingdao, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCAS Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, National Laboratory for Marine Science and Technology, Qingdao, ChinaMicroRNAs (miRNAs) are endogenous small non-coding RNAs that constitute a broad layer of gene regulation at both transcriptional and post-transcriptional levels from prokaryotes to eukaryotes. In embryonic development, they regulate the complex gene expression associated with the complexity of embryogenesis. There is little information about miRNAs in the red claw crayfish (Cherax quadricarinatus), an important commercial species and a potential biological model. In the present study, miRNAs and their target genes were identified during three embryonic developmental stages of C. quadricarinatus. Nineteen known miRNAs and 331 novel ones belonging to 50 miRNA families were obtained. A total of 113 differentially expressed miRNAs were identified, and 2,575 target genes were predicted, of which 1,257 were annotated. Additionally, 63 target genes of 9 miRNAs in C. quadricarinatus were found to be related to embryonic development. For example, miR-10 and its target genes may regulate the nervous system development and body segmentation and miR-2788 may regulate cell proliferation to impact embryonic development. Moreover, miR-28 (target gene tutl), miR-50 (target gene fbx5), and miR-1260b (target gene sif) may co-regulate eye development of embryonic C. quadricarinatus. These miRNAs together with their target genes constitute a network for regulating the development of tissues and organs in the embryo of C. quadricarinatus. Our results lay a foundation for further study on the fundamental molecular and developmental mechanism of crustacean embryogenesis.https://www.frontiersin.org/article/10.3389/fphys.2020.00878/fullCherax quadricarinatusembryonic developmenteye pigments forming stageprepare-hatching stagelarvaemicroRNA |
| spellingShingle | Yan Wang Yan Wang Baojie Wang Xuqing Shao Mei Liu Keyong Jiang Mengqiang Wang Mengqiang Wang Lei Wang Lei Wang Lei Wang Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus Frontiers in Physiology Cherax quadricarinatus embryonic development eye pigments forming stage prepare-hatching stage larvae microRNA |
| title | Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus |
| title_full | Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus |
| title_fullStr | Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus |
| title_full_unstemmed | Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus |
| title_short | Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw Crayfish Cherax quadricarinatus |
| title_sort | identification and profiling of micrornas during embryogenesis in the red claw crayfish cherax quadricarinatus |
| topic | Cherax quadricarinatus embryonic development eye pigments forming stage prepare-hatching stage larvae microRNA |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00878/full |
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