Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients
Abstract Heavy metal levels appear to be associated with low bone mineral density (BMD) and the consequent osteoporosis risk, but the relationship with the disease has not been clearly defined. The altered expression pattern of numerous genes, including detoxifying genes, seems to play a pivotal rol...
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| Format: | Article |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-37410-8 |
| _version_ | 1797789887373508608 |
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| author | V. V. Visconti B. Gasperini C. Greggi B. Battistini A. Messina M. Renzi K. Bakhtafrouz R. Iundusi A. Botta L. Palombi U. Tarantino |
| author_facet | V. V. Visconti B. Gasperini C. Greggi B. Battistini A. Messina M. Renzi K. Bakhtafrouz R. Iundusi A. Botta L. Palombi U. Tarantino |
| author_sort | V. V. Visconti |
| collection | DOAJ |
| description | Abstract Heavy metal levels appear to be associated with low bone mineral density (BMD) and the consequent osteoporosis risk, but the relationship with the disease has not been clearly defined. The altered expression pattern of numerous genes, including detoxifying genes, seems to play a pivotal role in this context, leading to increased susceptibility to several diseases, including osteoporosis. The purpose of this study is to analyse circulating heavy metals levels and the expression of detoxifying genes in osteoporotic patients (OPs, n = 31), compared with healthy subjects (CTRs, n = 32). Heavy metals concentration in plasma samples was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and the subsequent expression analysis of NAD(P)H quinone dehydrogenase 1 (NQO1), Catalase (CAT), and Metallothionein 1E (MT1E) genes in Peripheral Blood Mononuclear Cells (PBMCs) was assessed by real-time polymerase chain reaction (qRT-PCR). Copper (Cu), mercury (Hg), molybdenum (Mo) and lead (Pb) were found to be significantly higher in the plasma of OPs compared to CTRs. Analysis of the expression levels of detoxifying genes showed a significant decrease in CAT and MT1E in OP group. In addition, Cu correlated positively with the expression levels of both CAT and MT1E in CTRs group and MT1E in OPs. This study shows an increased circulating concentration of certain metals combined with an altered expression pattern of detoxifying genes in OPs, highlighting a novel aspect to be investigated in order to better characterize the role of metals in the pathogenesis of osteoporosis. |
| first_indexed | 2024-03-13T01:56:59Z |
| format | Article |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T01:56:59Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fdb0fe2329fc4360ad0739aa4f1ba0cb2023-07-02T11:12:39ZengNature PortfolioScientific Reports2045-23222023-06-011311710.1038/s41598-023-37410-8Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patientsV. V. Visconti0B. Gasperini1C. Greggi2B. Battistini3A. Messina4M. Renzi5K. Bakhtafrouz6R. Iundusi7A. Botta8L. Palombi9U. Tarantino10Department of Clinical Sciences and Translational Medicine, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataDepartment of Clinical Sciences and Translational Medicine, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataDepartment of Orthopaedics and Traumatology, “Policlinico Tor Vergata” FoundationDepartment of Orthopaedics and Traumatology, “Policlinico Tor Vergata” FoundationDepartment of Clinical Sciences and Translational Medicine, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataDepartment of Biomedicine and Prevention, University of Rome Tor VergataDepartment of Clinical Sciences and Translational Medicine, University of Rome Tor VergataAbstract Heavy metal levels appear to be associated with low bone mineral density (BMD) and the consequent osteoporosis risk, but the relationship with the disease has not been clearly defined. The altered expression pattern of numerous genes, including detoxifying genes, seems to play a pivotal role in this context, leading to increased susceptibility to several diseases, including osteoporosis. The purpose of this study is to analyse circulating heavy metals levels and the expression of detoxifying genes in osteoporotic patients (OPs, n = 31), compared with healthy subjects (CTRs, n = 32). Heavy metals concentration in plasma samples was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and the subsequent expression analysis of NAD(P)H quinone dehydrogenase 1 (NQO1), Catalase (CAT), and Metallothionein 1E (MT1E) genes in Peripheral Blood Mononuclear Cells (PBMCs) was assessed by real-time polymerase chain reaction (qRT-PCR). Copper (Cu), mercury (Hg), molybdenum (Mo) and lead (Pb) were found to be significantly higher in the plasma of OPs compared to CTRs. Analysis of the expression levels of detoxifying genes showed a significant decrease in CAT and MT1E in OP group. In addition, Cu correlated positively with the expression levels of both CAT and MT1E in CTRs group and MT1E in OPs. This study shows an increased circulating concentration of certain metals combined with an altered expression pattern of detoxifying genes in OPs, highlighting a novel aspect to be investigated in order to better characterize the role of metals in the pathogenesis of osteoporosis.https://doi.org/10.1038/s41598-023-37410-8 |
| spellingShingle | V. V. Visconti B. Gasperini C. Greggi B. Battistini A. Messina M. Renzi K. Bakhtafrouz R. Iundusi A. Botta L. Palombi U. Tarantino Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients Scientific Reports |
| title | Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| title_full | Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| title_fullStr | Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| title_full_unstemmed | Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| title_short | Plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| title_sort | plasma heavy metal levels correlate with deregulated gene expression of detoxifying enzymes in osteoporotic patients |
| url | https://doi.org/10.1038/s41598-023-37410-8 |
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