Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications
Owing to retained hepatic phenotypes and functions, human three-dimensional (3D) hepatic models established with diverse hepatic cell types are thought to recoup the gaps in drug development and disease modeling limited by a conventional two-dimensional (2D) cell culture system and species-specific...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.730008/full |
| _version_ | 1818904613035704320 |
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| author | Qianqian Xu |
| author_facet | Qianqian Xu |
| author_sort | Qianqian Xu |
| collection | DOAJ |
| description | Owing to retained hepatic phenotypes and functions, human three-dimensional (3D) hepatic models established with diverse hepatic cell types are thought to recoup the gaps in drug development and disease modeling limited by a conventional two-dimensional (2D) cell culture system and species-specific variability in drug metabolizing enzymes and transporters. Primary human hepatocytes, human hepatic cancer cell lines, and human stem cell–derived hepatocyte-like cells are three main hepatic cell types used in current models and exhibit divergent hepatic phenotypes. Primary human hepatocytes derived from healthy hepatic parenchyma resemble in vivo–like genetic and metabolic profiling. Human hepatic cancer cell lines are unlimitedly reproducible and tumorigenic. Stem cell–derived hepatocyte-like cells derived from patients are promising to retain the donor’s genetic background. It has been suggested in some studies that unique properties of cell types endue them with benefits in different research fields of in vitro 3D modeling paradigm. For instance, the primary human hepatocyte was thought to be the gold standard for hepatotoxicity study, and stem cell–derived hepatocyte-like cells have taken a main role in personalized medicine and regenerative medicine. However, the comprehensive review focuses on the hepatic cell type variety, and corresponding applications in 3D models are sparse. Therefore, this review summarizes the characteristics of different cell types and discusses opportunities of different cell types in drug development, liver disease modeling, and liver transplantation. |
| first_indexed | 2024-12-19T21:10:13Z |
| format | Article |
| id | doaj.art-fdb35dc19eee4da4874dfb0bb23f444e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-19T21:10:13Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-fdb35dc19eee4da4874dfb0bb23f444e2022-12-21T20:05:31ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-09-01910.3389/fbioe.2021.730008730008Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding ApplicationsQianqian XuOwing to retained hepatic phenotypes and functions, human three-dimensional (3D) hepatic models established with diverse hepatic cell types are thought to recoup the gaps in drug development and disease modeling limited by a conventional two-dimensional (2D) cell culture system and species-specific variability in drug metabolizing enzymes and transporters. Primary human hepatocytes, human hepatic cancer cell lines, and human stem cell–derived hepatocyte-like cells are three main hepatic cell types used in current models and exhibit divergent hepatic phenotypes. Primary human hepatocytes derived from healthy hepatic parenchyma resemble in vivo–like genetic and metabolic profiling. Human hepatic cancer cell lines are unlimitedly reproducible and tumorigenic. Stem cell–derived hepatocyte-like cells derived from patients are promising to retain the donor’s genetic background. It has been suggested in some studies that unique properties of cell types endue them with benefits in different research fields of in vitro 3D modeling paradigm. For instance, the primary human hepatocyte was thought to be the gold standard for hepatotoxicity study, and stem cell–derived hepatocyte-like cells have taken a main role in personalized medicine and regenerative medicine. However, the comprehensive review focuses on the hepatic cell type variety, and corresponding applications in 3D models are sparse. Therefore, this review summarizes the characteristics of different cell types and discusses opportunities of different cell types in drug development, liver disease modeling, and liver transplantation.https://www.frontiersin.org/articles/10.3389/fbioe.2021.730008/fullin vitro 3D modeldrug developmentliver diseasehepatocyte transplantationhepatic cell types |
| spellingShingle | Qianqian Xu Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications Frontiers in Bioengineering and Biotechnology in vitro 3D model drug development liver disease hepatocyte transplantation hepatic cell types |
| title | Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications |
| title_full | Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications |
| title_fullStr | Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications |
| title_full_unstemmed | Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications |
| title_short | Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications |
| title_sort | human three dimensional hepatic models cell type variety and corresponding applications |
| topic | in vitro 3D model drug development liver disease hepatocyte transplantation hepatic cell types |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.730008/full |
| work_keys_str_mv | AT qianqianxu humanthreedimensionalhepaticmodelscelltypevarietyandcorrespondingapplications |