A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). U...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007158 |
| _version_ | 1797980858307575808 |
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| author | Benjamin Louis Sievers Terri Gelbart Gene S. Tan |
| author_facet | Benjamin Louis Sievers Terri Gelbart Gene S. Tan |
| author_sort | Benjamin Louis Sievers |
| collection | DOAJ |
| description | Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability.For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T06:00:23Z |
| format | Article |
| id | doaj.art-fdb4223a59ff40fda1396cf44b267e5b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T06:00:23Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-fdb4223a59ff40fda1396cf44b267e5b2022-12-22T04:41:43ZengElsevierSTAR Protocols2666-16672022-12-0134101835A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assayBenjamin Louis Sievers0Terri Gelbart1Gene S. Tan2J. Craig Venter Institute, La Jolla, CA 92037, USAJ. Craig Venter Institute, La Jolla, CA 92037, USAJ. Craig Venter Institute, La Jolla, CA 92037, USA; Division of Infectious Diseases, Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA; Corresponding authorSummary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability.For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007158Cell-based AssaysHigh Throughput ScreeningMicrobiologyAntibody |
| spellingShingle | Benjamin Louis Sievers Terri Gelbart Gene S. Tan A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay STAR Protocols Cell-based Assays High Throughput Screening Microbiology Antibody |
| title | A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay |
| title_full | A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay |
| title_fullStr | A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay |
| title_full_unstemmed | A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay |
| title_short | A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay |
| title_sort | high throughput sars cov 2 pseudovirus multiplex neutralization assay |
| topic | Cell-based Assays High Throughput Screening Microbiology Antibody |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007158 |
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