Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis
Syphilis, a chronic multisystemic disease caused by spirochete Treponema pallidum subspecies pallidum infection, continues to be a serious global health problem and congenital syphilis remains a major cause of adverse outcomes in pregnancy in developing countries. The development of an effective vac...
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| Format: | Article |
| Idioma: | English |
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Frontiers Media S.A.
2023-03-01
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| Col·lecció: | Frontiers in Immunology |
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| Accés en línia: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1130593/full |
| _version_ | 1827991415019274240 |
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| author | Yuxing He Yuxing He Dejun Chen Dejun Chen Yue Fu Yue Fu Xinzhuo Huo Xinzhuo Huo Feijun Zhao Feijun Zhao Ling Yao Xiuping Zhou Pengfei Qi Haoquan Yin Longgu Cao Hui Ling Hui Ling Tiebing Zeng Tiebing Zeng |
| author_facet | Yuxing He Yuxing He Dejun Chen Dejun Chen Yue Fu Yue Fu Xinzhuo Huo Xinzhuo Huo Feijun Zhao Feijun Zhao Ling Yao Xiuping Zhou Pengfei Qi Haoquan Yin Longgu Cao Hui Ling Hui Ling Tiebing Zeng Tiebing Zeng |
| author_sort | Yuxing He |
| collection | DOAJ |
| description | Syphilis, a chronic multisystemic disease caused by spirochete Treponema pallidum subspecies pallidum infection, continues to be a serious global health problem and congenital syphilis remains a major cause of adverse outcomes in pregnancy in developing countries. The development of an effective vaccine is the most cost-effective way to eliminate syphilis, but so far has been elusive. Here, we evaluated the immunogenicity and protective efficacy of Tp0954, a T. pallidum placental adhesin, as a potential vaccine candidate in a New Zealand White rabbit model of experimental syphilis. Animals immunized with recombinant Tp0954 (rTp0954) produced high titers of Tp0954-specific serum IgG, high levels of IFN-γ from splenocytes and specific splenocyte proliferation response when compared to control animals immunized with PBS and Freund’s adjuvant (FA). Furthermore, rTp0954 immunization significantly delayed the development of cutaneous lesions, promoted inflammatory cellular infiltration at the primary lesion sites, as well as inhibited T. pallidum dissemination to distal tissues or organs when compared with that of the control animals. In addition, the naïve rabbits receiving popliteal lymph nodes from Tp0954-immunized, T. pallidum-challenged animals were not infected by T. pallidum, confirming sterile immunity. These findings suggest that Tp0954 is a potential vaccine candidate against syphilis. |
| first_indexed | 2024-04-10T00:50:21Z |
| format | Article |
| id | doaj.art-fdb6acdea1e94e1f8329c2204db313b6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T00:50:21Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-fdb6acdea1e94e1f8329c2204db313b62023-03-13T13:54:05ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.11305931130593Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilisYuxing He0Yuxing He1Dejun Chen2Dejun Chen3Yue Fu4Yue Fu5Xinzhuo Huo6Xinzhuo Huo7Feijun Zhao8Feijun Zhao9Ling Yao10Xiuping Zhou11Pengfei Qi12Haoquan Yin13Longgu Cao14Hui Ling15Hui Ling16Tiebing Zeng17Tiebing Zeng18Institution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaDepartment of Laboratory Medicine, Changsha Health Vocational College, Changsha, ChinaDepartment of Laboratory Medicine, Changsha Health Vocational College, Changsha, ChinaDepartment of Clinical Medical undergraduates, Hengyang Medical School, University of South China, Hengyang, ChinaDepartment of Clinical Medical undergraduates, Hengyang Medical School, University of South China, Hengyang, ChinaCollege of Medical Imaging and Inspection, Xiangnan University, Chenzhou, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaHunan Province Key Laboratory of Tumor Cellular and Molecular Pathology, Cancer Research Institute, Hengyang Medical School, University of South China, Hengyang, ChinaInstitution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, ChinaHunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, ChinaSyphilis, a chronic multisystemic disease caused by spirochete Treponema pallidum subspecies pallidum infection, continues to be a serious global health problem and congenital syphilis remains a major cause of adverse outcomes in pregnancy in developing countries. The development of an effective vaccine is the most cost-effective way to eliminate syphilis, but so far has been elusive. Here, we evaluated the immunogenicity and protective efficacy of Tp0954, a T. pallidum placental adhesin, as a potential vaccine candidate in a New Zealand White rabbit model of experimental syphilis. Animals immunized with recombinant Tp0954 (rTp0954) produced high titers of Tp0954-specific serum IgG, high levels of IFN-γ from splenocytes and specific splenocyte proliferation response when compared to control animals immunized with PBS and Freund’s adjuvant (FA). Furthermore, rTp0954 immunization significantly delayed the development of cutaneous lesions, promoted inflammatory cellular infiltration at the primary lesion sites, as well as inhibited T. pallidum dissemination to distal tissues or organs when compared with that of the control animals. In addition, the naïve rabbits receiving popliteal lymph nodes from Tp0954-immunized, T. pallidum-challenged animals were not infected by T. pallidum, confirming sterile immunity. These findings suggest that Tp0954 is a potential vaccine candidate against syphilis.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1130593/fullTreponema pallidumTp0954placental adhesinvaccineimmunoprotectiondissemination |
| spellingShingle | Yuxing He Yuxing He Dejun Chen Dejun Chen Yue Fu Yue Fu Xinzhuo Huo Xinzhuo Huo Feijun Zhao Feijun Zhao Ling Yao Xiuping Zhou Pengfei Qi Haoquan Yin Longgu Cao Hui Ling Hui Ling Tiebing Zeng Tiebing Zeng Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis Frontiers in Immunology Treponema pallidum Tp0954 placental adhesin vaccine immunoprotection dissemination |
| title | Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis |
| title_full | Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis |
| title_fullStr | Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis |
| title_full_unstemmed | Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis |
| title_short | Immunization with Tp0954, an adhesin of Treponema pallidum, provides protective efficacy in the rabbit model of experimental syphilis |
| title_sort | immunization with tp0954 an adhesin of treponema pallidum provides protective efficacy in the rabbit model of experimental syphilis |
| topic | Treponema pallidum Tp0954 placental adhesin vaccine immunoprotection dissemination |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1130593/full |
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