Is reliance on an inaccurate genome sequence sabotaging your experiments?
Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation...
Main Authors: | , |
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Format: | Article |
Language: | English |
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Public Library of Science (PLoS)
2019-09-01
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Series: | PLoS Pathogens |
Online Access: | https://doi.org/10.1371/journal.ppat.1007901 |
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author | Rodrigo P Baptista Jessica C Kissinger |
author_facet | Rodrigo P Baptista Jessica C Kissinger |
author_sort | Rodrigo P Baptista |
collection | DOAJ |
description | Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result. |
first_indexed | 2024-12-18T01:08:26Z |
format | Article |
id | doaj.art-fdbd2829d3094472b1e1beff94f7a3de |
institution | Directory Open Access Journal |
issn | 1553-7366 1553-7374 |
language | English |
last_indexed | 2024-12-18T01:08:26Z |
publishDate | 2019-09-01 |
publisher | Public Library of Science (PLoS) |
record_format | Article |
series | PLoS Pathogens |
spelling | doaj.art-fdbd2829d3094472b1e1beff94f7a3de2022-12-21T21:26:10ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742019-09-01159e100790110.1371/journal.ppat.1007901Is reliance on an inaccurate genome sequence sabotaging your experiments?Rodrigo P BaptistaJessica C KissingerAdvances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.https://doi.org/10.1371/journal.ppat.1007901 |
spellingShingle | Rodrigo P Baptista Jessica C Kissinger Is reliance on an inaccurate genome sequence sabotaging your experiments? PLoS Pathogens |
title | Is reliance on an inaccurate genome sequence sabotaging your experiments? |
title_full | Is reliance on an inaccurate genome sequence sabotaging your experiments? |
title_fullStr | Is reliance on an inaccurate genome sequence sabotaging your experiments? |
title_full_unstemmed | Is reliance on an inaccurate genome sequence sabotaging your experiments? |
title_short | Is reliance on an inaccurate genome sequence sabotaging your experiments? |
title_sort | is reliance on an inaccurate genome sequence sabotaging your experiments |
url | https://doi.org/10.1371/journal.ppat.1007901 |
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