Is reliance on an inaccurate genome sequence sabotaging your experiments?

Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation...

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Main Authors: Rodrigo P Baptista, Jessica C Kissinger
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-09-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1007901
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author Rodrigo P Baptista
Jessica C Kissinger
author_facet Rodrigo P Baptista
Jessica C Kissinger
author_sort Rodrigo P Baptista
collection DOAJ
description Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.
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spelling doaj.art-fdbd2829d3094472b1e1beff94f7a3de2022-12-21T21:26:10ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742019-09-01159e100790110.1371/journal.ppat.1007901Is reliance on an inaccurate genome sequence sabotaging your experiments?Rodrigo P BaptistaJessica C KissingerAdvances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.https://doi.org/10.1371/journal.ppat.1007901
spellingShingle Rodrigo P Baptista
Jessica C Kissinger
Is reliance on an inaccurate genome sequence sabotaging your experiments?
PLoS Pathogens
title Is reliance on an inaccurate genome sequence sabotaging your experiments?
title_full Is reliance on an inaccurate genome sequence sabotaging your experiments?
title_fullStr Is reliance on an inaccurate genome sequence sabotaging your experiments?
title_full_unstemmed Is reliance on an inaccurate genome sequence sabotaging your experiments?
title_short Is reliance on an inaccurate genome sequence sabotaging your experiments?
title_sort is reliance on an inaccurate genome sequence sabotaging your experiments
url https://doi.org/10.1371/journal.ppat.1007901
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