First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia
Abstract Background Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels. Objective The aim of this study was to estimate the molecular detection of Neospora caninum in tissue...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-09-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.901 |
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| author | Yosra Amdouni Imen abedennebi Safa Amairia Amara Abdelkader Walid Chandoul Mohamed Gharbi |
| author_facet | Yosra Amdouni Imen abedennebi Safa Amairia Amara Abdelkader Walid Chandoul Mohamed Gharbi |
| author_sort | Yosra Amdouni |
| collection | DOAJ |
| description | Abstract Background Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels. Objective The aim of this study was to estimate the molecular detection of Neospora caninum in tissues of naturally infected camelids. Methods Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and Médenine regions were collected (n = 140 samples). PCR was used to amplify and detect N. caninum DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect Neospora spp. cysts, but no lesions were observed. Results The overall molecular detection of N. caninum in camelids was 34.3% (12/35). The highest molecular detection of N. caninum was recorded in animals of more than 3 years old (6/9) and in animals aged between 1 and 3 years old (4/12). Whilst, the lowest molecular detection (2/14) was observed in animals 1 year or younger (p = 0.035). There were no significant differences in molecular detection of N. caninum according to both locality and gender (p > 0.05). Similarly, there was no difference of prevalence between different anatomical locations. Comparison of the partial sequences of the ITS1 gene revealed 100–95.5% similarity among our N. caninum amplicon (MW551566) and those deposited in GenBank. Conclusion These results highlight the presence of a risk infection by N. caninum in camels. For preventing N. caninum infection further studies are needed to improve our knowledge about the epidemiology of neosporosis in North Africa. |
| first_indexed | 2024-04-11T11:33:05Z |
| format | Article |
| id | doaj.art-fdc351ee3e8048deb552a3a80f47696d |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-11T11:33:05Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-fdc351ee3e8048deb552a3a80f47696d2022-12-22T04:26:05ZengWileyVeterinary Medicine and Science2053-10952022-09-01852241224710.1002/vms3.901First molecular detection of Neospora caninum from naturally infected slaughtered camels in TunisiaYosra Amdouni0Imen abedennebi1Safa Amairia2Amara Abdelkader3Walid Chandoul4Mohamed Gharbi5Laboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Supérieur Agricoles École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet TunisiaLaboratoire d'anatomie Pathologique École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet TunisiaLaboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Supérieur Agricoles École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet TunisiaLaboratoire d'anatomie Pathologique École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet TunisiaMinistry of Agriculture Water Resources and Maritime Fisheries, Arrondissement de Production Animale de Médenine Médenine TunisiaLaboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Supérieur Agricoles École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet TunisiaAbstract Background Neospora caninum has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels. Objective The aim of this study was to estimate the molecular detection of Neospora caninum in tissues of naturally infected camelids. Methods Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and Médenine regions were collected (n = 140 samples). PCR was used to amplify and detect N. caninum DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect Neospora spp. cysts, but no lesions were observed. Results The overall molecular detection of N. caninum in camelids was 34.3% (12/35). The highest molecular detection of N. caninum was recorded in animals of more than 3 years old (6/9) and in animals aged between 1 and 3 years old (4/12). Whilst, the lowest molecular detection (2/14) was observed in animals 1 year or younger (p = 0.035). There were no significant differences in molecular detection of N. caninum according to both locality and gender (p > 0.05). Similarly, there was no difference of prevalence between different anatomical locations. Comparison of the partial sequences of the ITS1 gene revealed 100–95.5% similarity among our N. caninum amplicon (MW551566) and those deposited in GenBank. Conclusion These results highlight the presence of a risk infection by N. caninum in camels. For preventing N. caninum infection further studies are needed to improve our knowledge about the epidemiology of neosporosis in North Africa.https://doi.org/10.1002/vms3.901camelidsmolecular detectionNeospora caninumPCRSouth Tunisia |
| spellingShingle | Yosra Amdouni Imen abedennebi Safa Amairia Amara Abdelkader Walid Chandoul Mohamed Gharbi First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia Veterinary Medicine and Science camelids molecular detection Neospora caninum PCR South Tunisia |
| title | First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia |
| title_full | First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia |
| title_fullStr | First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia |
| title_full_unstemmed | First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia |
| title_short | First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia |
| title_sort | first molecular detection of neospora caninum from naturally infected slaughtered camels in tunisia |
| topic | camelids molecular detection Neospora caninum PCR South Tunisia |
| url | https://doi.org/10.1002/vms3.901 |
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