Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon
Different species of edible seed watermelons (Citrullus spp.) are cultivated in Asia and Africa for their colorful nutritious seeds. Consumer preference varies for watermelon seed coat color. Therefore, it is an important consideration for watermelon breeders. In 1940s, a genetic model of four genes...
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00788/full |
| _version_ | 1811214759392968704 |
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| author | Lucky Paudel Josh Clevenger Cecilia McGregor Cecilia McGregor |
| author_facet | Lucky Paudel Josh Clevenger Cecilia McGregor Cecilia McGregor |
| author_sort | Lucky Paudel |
| collection | DOAJ |
| description | Different species of edible seed watermelons (Citrullus spp.) are cultivated in Asia and Africa for their colorful nutritious seeds. Consumer preference varies for watermelon seed coat color. Therefore, it is an important consideration for watermelon breeders. In 1940s, a genetic model of four genes, R, T, W and D, was proposed to elucidate the inheritance of seed coat color in watermelon. In this study, we developed three segregating F2 populations: Sugar Baby (dotted black seed, RRTTWW) × plant introduction (PI) 482379 (green seed, rrTTWW), Charleston Gray (dotted black seed, RRTTWW) × PI 189225 (red seed, rrttWW), and Charleston Gray (dotted black seed, RRTTWWdd) × UGA147 (clump seed, RRTTwwDD) to re-examine the four-gene model and to map the four genes. In the dotted black × green population, the dotted black seed coat color (R_) is dominant to green seed coat color (rr). In the dotted black × red population, the dominant dotted black seed coat color and the recessive red seed coat color segregate for the R and T genes, where the R gene is dominantly epistatic to the T gene. However, the inheritance of the T locus did not fit the four-gene model, thus we named it T1. In the dotted black × clump population, the clump seed coat color and the dotted black seed coat color segregate for W and D, where D is recessively epistatic to W. The R, T1, W, and D loci were mapped on chromosomes 3, 5, 6, and 8, respectively, using QTL-seq and genotyping-by-sequencing (GBS). Kompetitive Allele Specific PCR (KASP™) assays and SNP markers linked to the four loci were developed to facilitate maker-assisted selection (MAS) for watermelon seed coat color. |
| first_indexed | 2024-04-12T06:10:06Z |
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| id | doaj.art-fdc8491a0c5844fdbb4ded73e0b4b082 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T06:10:06Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-fdc8491a0c5844fdbb4ded73e0b4b0822022-12-22T03:44:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-06-011010.3389/fpls.2019.00788460813Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in WatermelonLucky Paudel0Josh Clevenger1Cecilia McGregor2Cecilia McGregor3Institute for Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United StatesInstitute for Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United StatesInstitute for Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA, United StatesDepartment of Horticulture, University of Georgia, Athens, GA, United StatesDifferent species of edible seed watermelons (Citrullus spp.) are cultivated in Asia and Africa for their colorful nutritious seeds. Consumer preference varies for watermelon seed coat color. Therefore, it is an important consideration for watermelon breeders. In 1940s, a genetic model of four genes, R, T, W and D, was proposed to elucidate the inheritance of seed coat color in watermelon. In this study, we developed three segregating F2 populations: Sugar Baby (dotted black seed, RRTTWW) × plant introduction (PI) 482379 (green seed, rrTTWW), Charleston Gray (dotted black seed, RRTTWW) × PI 189225 (red seed, rrttWW), and Charleston Gray (dotted black seed, RRTTWWdd) × UGA147 (clump seed, RRTTwwDD) to re-examine the four-gene model and to map the four genes. In the dotted black × green population, the dotted black seed coat color (R_) is dominant to green seed coat color (rr). In the dotted black × red population, the dominant dotted black seed coat color and the recessive red seed coat color segregate for the R and T genes, where the R gene is dominantly epistatic to the T gene. However, the inheritance of the T locus did not fit the four-gene model, thus we named it T1. In the dotted black × clump population, the clump seed coat color and the dotted black seed coat color segregate for W and D, where D is recessively epistatic to W. The R, T1, W, and D loci were mapped on chromosomes 3, 5, 6, and 8, respectively, using QTL-seq and genotyping-by-sequencing (GBS). Kompetitive Allele Specific PCR (KASP™) assays and SNP markers linked to the four loci were developed to facilitate maker-assisted selection (MAS) for watermelon seed coat color.https://www.frontiersin.org/article/10.3389/fpls.2019.00788/fullCitrullus lanatusCitrullus amarusedible seed watermelonseed coat colorQTL-seqKASP™ assay |
| spellingShingle | Lucky Paudel Josh Clevenger Cecilia McGregor Cecilia McGregor Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon Frontiers in Plant Science Citrullus lanatus Citrullus amarus edible seed watermelon seed coat color QTL-seq KASP™ assay |
| title | Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon |
| title_full | Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon |
| title_fullStr | Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon |
| title_full_unstemmed | Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon |
| title_short | Chromosomal Locations and Interactions of Four Loci Associated With Seed Coat Color in Watermelon |
| title_sort | chromosomal locations and interactions of four loci associated with seed coat color in watermelon |
| topic | Citrullus lanatus Citrullus amarus edible seed watermelon seed coat color QTL-seq KASP™ assay |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.00788/full |
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