Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq
The interleukin-1 family has multifaceted roles in men٫s reproductive syste. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of V...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2021-09-01
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| Series: | Baghdad Science Journal |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3856 |
| _version_ | 1818822757567168512 |
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| author | Yassin Kareem Amin Ashti M. Said Ahmed A. Al-Naqshbandi Saeed Ghulam |
| author_facet | Yassin Kareem Amin Ashti M. Said Ahmed A. Al-Naqshbandi Saeed Ghulam |
| author_sort | Yassin Kareem Amin |
| collection | DOAJ |
| description | The interleukin-1 family has multifaceted roles in men٫s reproductive syste. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two alleles, namely IL-1RN1 allele corresponding to 410bp fragment and IL-11RN2 that corresponding to 240bp fragments, are a marker for human men infertility, detected by PCR technique. The results delineated a high frequency of IL-1RN2 allelic gene variants (26%), and two VNTR allelic gene variants carriers IL-1RN1 and IL-1RN2 (16%) among infertile men with significant impacts on sperm motility and morphology (P< 0.000-0.002) respectively. This prospective study inKurdistan region (Erbil –Iraq) defined a significant impact of VNTR polymorphism of IL-1RN gene in the etiology of men infertility especially on sperm motility and morphology; particularly carriers of IL-1RN2 allelic variants |
| first_indexed | 2024-12-18T23:29:09Z |
| format | Article |
| id | doaj.art-fdc8cdb2b55343688499e7dc8d95a8c9 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-12-18T23:29:09Z |
| publishDate | 2021-09-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-fdc8cdb2b55343688499e7dc8d95a8c92022-12-21T20:47:43ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862021-09-0118310.21123/bsj.2021.18.3.0438Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan IraqYassin Kareem Amin0Ashti M. Said1Ahmed A. Al-Naqshbandi2Saeed Ghulam3Hawler Medical University-Medical Research CenterMedical Research Center, Hawler Medical University, Erbil, Iraq Rizgary Hospital, Erbil, IraqCollege of Medicine , Hawler Medical University, Erbil, IraqThe interleukin-1 family has multifaceted roles in men٫s reproductive syste. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two alleles, namely IL-1RN1 allele corresponding to 410bp fragment and IL-11RN2 that corresponding to 240bp fragments, are a marker for human men infertility, detected by PCR technique. The results delineated a high frequency of IL-1RN2 allelic gene variants (26%), and two VNTR allelic gene variants carriers IL-1RN1 and IL-1RN2 (16%) among infertile men with significant impacts on sperm motility and morphology (P< 0.000-0.002) respectively. This prospective study inKurdistan region (Erbil –Iraq) defined a significant impact of VNTR polymorphism of IL-1RN gene in the etiology of men infertility especially on sperm motility and morphology; particularly carriers of IL-1RN2 allelic variantshttps://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3856 |
| spellingShingle | Yassin Kareem Amin Ashti M. Said Ahmed A. Al-Naqshbandi Saeed Ghulam Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq Baghdad Science Journal |
| title | Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq |
| title_full | Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq |
| title_fullStr | Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq |
| title_full_unstemmed | Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq |
| title_short | Interleukin-1 Receptor Antagonist (IL-1RN) Gene Variable Number Tandem Repeats (VNTR) Polymorphism Association in men Infertility in Erbil City /Kurdistan Iraq |
| title_sort | interleukin 1 receptor antagonist il 1rn gene variable number tandem repeats vntr polymorphism association in men infertility in erbil city kurdistan iraq |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3856 |
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