Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications
To explore the mitogenome characteristics and shed light on the phylogenetic relationships and molecular evolution of Drabescini species, we sequenced and analyzed the complete mitochondrial genome of two species including <i>Drabescus ineffectus</i> and <i>Roxasellana stellata<...
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MDPI AG
2020-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/8/534 |
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| author | Deliang Xu Tinghao Yu Yalin Zhang |
| author_facet | Deliang Xu Tinghao Yu Yalin Zhang |
| author_sort | Deliang Xu |
| collection | DOAJ |
| description | To explore the mitogenome characteristics and shed light on the phylogenetic relationships and molecular evolution of Drabescini species, we sequenced and analyzed the complete mitochondrial genome of two species including <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i>. The complete mitogenomes of <i>D. ineffectus</i> and <i>R. stellata</i> are circular, closed and double-stranded molecules with a total length of 15744 bp and 15361 bp, respectively. These two newly sequenced mitogenomes contain the typical 37 genes. Most protein-coding genes (PCGs) began with the start codon ATN and terminated with the terminal codon TAA or TAG, with an exception of a special initiation codon of <i>ND5</i>, which started with TTG, and an incomplete stop codon T-- was found in the <i>Cytb</i>, <i>COX2</i>, <i>ND1</i> and <i>ND4</i>. All tRNAs could be folded into the canonical cloverleaf secondary structure except for the <i>trnS1</i>, which lacks the DHU arm and is replaced by a simple loop. The multiple tandem repeat units were found in A + T-control region. The sliding window, Ka/Ks and genetic distance analyses indicated that the <i>ATP8</i> presents a high variability and fast evolutionary rate compared to other PCGs. Phylogenetic analyses based on three different datasets (PCG123, PCG12R and AA) using both Bayesian inference (BI) and maximum likelihood (ML) methods showed strong support for the monophyly of Drabescini. |
| first_indexed | 2024-03-10T17:24:56Z |
| format | Article |
| id | doaj.art-fdca451341de45eb9de77c97a8da4555 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T17:24:56Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-fdca451341de45eb9de77c97a8da45552023-11-20T10:14:05ZengMDPI AGInsects2075-44502020-08-0111853410.3390/insects11080534Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic ImplicationsDeliang Xu0Tinghao Yu1Yalin Zhang2Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Entomological Museum, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, ChinaTo explore the mitogenome characteristics and shed light on the phylogenetic relationships and molecular evolution of Drabescini species, we sequenced and analyzed the complete mitochondrial genome of two species including <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i>. The complete mitogenomes of <i>D. ineffectus</i> and <i>R. stellata</i> are circular, closed and double-stranded molecules with a total length of 15744 bp and 15361 bp, respectively. These two newly sequenced mitogenomes contain the typical 37 genes. Most protein-coding genes (PCGs) began with the start codon ATN and terminated with the terminal codon TAA or TAG, with an exception of a special initiation codon of <i>ND5</i>, which started with TTG, and an incomplete stop codon T-- was found in the <i>Cytb</i>, <i>COX2</i>, <i>ND1</i> and <i>ND4</i>. All tRNAs could be folded into the canonical cloverleaf secondary structure except for the <i>trnS1</i>, which lacks the DHU arm and is replaced by a simple loop. The multiple tandem repeat units were found in A + T-control region. The sliding window, Ka/Ks and genetic distance analyses indicated that the <i>ATP8</i> presents a high variability and fast evolutionary rate compared to other PCGs. Phylogenetic analyses based on three different datasets (PCG123, PCG12R and AA) using both Bayesian inference (BI) and maximum likelihood (ML) methods showed strong support for the monophyly of Drabescini.https://www.mdpi.com/2075-4450/11/8/534DeltocephalinaeDrabescinimitochondrial genomephylogeny |
| spellingShingle | Deliang Xu Tinghao Yu Yalin Zhang Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications Insects Deltocephalinae Drabescini mitochondrial genome phylogeny |
| title | Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications |
| title_full | Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications |
| title_fullStr | Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications |
| title_full_unstemmed | Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications |
| title_short | Characterization of the Complete Mitochondrial Genome of <i>Drabescus ineffectus</i> and <i>Roxasellana stellata</i> (Hemiptera: Cicadellidae: Deltocephalinae: Drabescini) and Their Phylogenetic Implications |
| title_sort | characterization of the complete mitochondrial genome of i drabescus ineffectus i and i roxasellana stellata i hemiptera cicadellidae deltocephalinae drabescini and their phylogenetic implications |
| topic | Deltocephalinae Drabescini mitochondrial genome phylogeny |
| url | https://www.mdpi.com/2075-4450/11/8/534 |
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