The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China
Abstract Background It has been performed worldwidely to explore the potential of animals that might be a reservoir for community associated human infections of Clostridioides difficile. Several genetically undistinguished PCR ribotypes of C. difficile from animals and human have been reported, illu...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01757-z |
| _version_ | 1818520123551514624 |
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| author | Wen-Zhu Zhang Wen-Ge Li Yu-Qing Liu Wen-Peng Gu Qing Zhang Hu Li Zheng-Jie Liu Xin Zhang Yuan Wu Jin-Xing Lu |
| author_facet | Wen-Zhu Zhang Wen-Ge Li Yu-Qing Liu Wen-Peng Gu Qing Zhang Hu Li Zheng-Jie Liu Xin Zhang Yuan Wu Jin-Xing Lu |
| author_sort | Wen-Zhu Zhang |
| collection | DOAJ |
| description | Abstract Background It has been performed worldwidely to explore the potential of animals that might be a reservoir for community associated human infections of Clostridioides difficile. Several genetically undistinguished PCR ribotypes of C. difficile from animals and human have been reported, illustrating potential transmission of C. difficile between them. Pig and calf were considered as the main origins of C. difficile with predominant RT078 and RT033, respectively. As more investigations involved, great diversity of molecular types from pig and calf were reported in Europe, North American and Australia. However, there were quite limited research on C. difficile isolates from meat animals in China, leading to non-comprehensive understanding of molecular epidemiology of C. difficile in China. Results A total of 55 C. difficile were isolated from 953 animal stool samples, within which 51 strains were from newborn dairy calf less than 7 days in Shandong Province. These isolates were divided into 3 STs and 6 RTs, of which ST11/RT126 was predominant type, and responsible for majority antibiotic resistance isolates. All the isolates were resistant to at least one tested antibiotics, however, only two multidrug resistant (MDR) isolates were identified. Furthermore, erythromycin (ERY) and clindamycin (CLI) were the two main resistant antibiotics. None of the isolates were resistant to vancomycin (VAN), metronidazole (MTZ), tetracycline (TET), and rifampin (RIF). Conclusions In this study, we analyzed the prevalence, molecular characters and antibiotic resistance of C. difficile from calf, sheep, chicken, and pig in China. Some unique features were found here: first, RT126 not RT078 were the dominant type from baby calf, and none isolates were got from pig; second, on the whole, isolates from animals display relative lower resistant rate to these 11 tested antibiotics, compared with isolates from human in China in our previous report. Our study helps to deep understanding the situation of C. difficile from economic animals in China, and to further study the potential transmission of C. difficile between meat animals and human. |
| first_indexed | 2024-12-11T01:33:10Z |
| format | Article |
| id | doaj.art-fdca80e166504425bdfcf4515b34c5a0 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T01:33:10Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-fdca80e166504425bdfcf4515b34c5a02022-12-22T01:25:18ZengBMCBMC Microbiology1471-21802020-03-012011710.1186/s12866-020-01757-zThe molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in ChinaWen-Zhu Zhang0Wen-Ge Li1Yu-Qing Liu2Wen-Peng Gu3Qing Zhang4Hu Li5Zheng-Jie Liu6Xin Zhang7Yuan Wu8Jin-Xing Lu9State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlInstitute of Animal Science and Veterinary Medicine, Shandong academy of agricultural SciencesDepartment of Acute Infectious Diseases Control and Prevention, Yunnan Provincial Centre for Disease Control and PreventionInstitute of Animal Science and Veterinary Medicine, Shandong academy of agricultural SciencesState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Prevention and ControlAbstract Background It has been performed worldwidely to explore the potential of animals that might be a reservoir for community associated human infections of Clostridioides difficile. Several genetically undistinguished PCR ribotypes of C. difficile from animals and human have been reported, illustrating potential transmission of C. difficile between them. Pig and calf were considered as the main origins of C. difficile with predominant RT078 and RT033, respectively. As more investigations involved, great diversity of molecular types from pig and calf were reported in Europe, North American and Australia. However, there were quite limited research on C. difficile isolates from meat animals in China, leading to non-comprehensive understanding of molecular epidemiology of C. difficile in China. Results A total of 55 C. difficile were isolated from 953 animal stool samples, within which 51 strains were from newborn dairy calf less than 7 days in Shandong Province. These isolates were divided into 3 STs and 6 RTs, of which ST11/RT126 was predominant type, and responsible for majority antibiotic resistance isolates. All the isolates were resistant to at least one tested antibiotics, however, only two multidrug resistant (MDR) isolates were identified. Furthermore, erythromycin (ERY) and clindamycin (CLI) were the two main resistant antibiotics. None of the isolates were resistant to vancomycin (VAN), metronidazole (MTZ), tetracycline (TET), and rifampin (RIF). Conclusions In this study, we analyzed the prevalence, molecular characters and antibiotic resistance of C. difficile from calf, sheep, chicken, and pig in China. Some unique features were found here: first, RT126 not RT078 were the dominant type from baby calf, and none isolates were got from pig; second, on the whole, isolates from animals display relative lower resistant rate to these 11 tested antibiotics, compared with isolates from human in China in our previous report. Our study helps to deep understanding the situation of C. difficile from economic animals in China, and to further study the potential transmission of C. difficile between meat animals and human.http://link.springer.com/article/10.1186/s12866-020-01757-zClostridioides difficileEconomic animalAntibiotics resistance |
| spellingShingle | Wen-Zhu Zhang Wen-Ge Li Yu-Qing Liu Wen-Peng Gu Qing Zhang Hu Li Zheng-Jie Liu Xin Zhang Yuan Wu Jin-Xing Lu The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China BMC Microbiology Clostridioides difficile Economic animal Antibiotics resistance |
| title | The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China |
| title_full | The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China |
| title_fullStr | The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China |
| title_full_unstemmed | The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China |
| title_short | The molecular characters and antibiotic resistance of Clostridioides difficile from economic animals in China |
| title_sort | molecular characters and antibiotic resistance of clostridioides difficile from economic animals in china |
| topic | Clostridioides difficile Economic animal Antibiotics resistance |
| url | http://link.springer.com/article/10.1186/s12866-020-01757-z |
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