Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+
Abstract Background The vaginal microbiota has been reported to be associated with HPV infection and cervical cancer. This study was performed to compare the vaginal microbiota at two timepoints in women performing self-sampling and had a persistent or transient HPV16 infection. The women were teste...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01420-z |
| _version_ | 1819012841051521024 |
|---|---|
| author | Malin Berggrund Inger Gustavsson Riina Aarnio Julia Hedlund Lindberg Karin Sanner Ingrid Wikström Stefan Enroth Ignas Bunikis Matts Olovsson Ulf Gyllensten |
| author_facet | Malin Berggrund Inger Gustavsson Riina Aarnio Julia Hedlund Lindberg Karin Sanner Ingrid Wikström Stefan Enroth Ignas Bunikis Matts Olovsson Ulf Gyllensten |
| author_sort | Malin Berggrund |
| collection | DOAJ |
| description | Abstract Background The vaginal microbiota has been reported to be associated with HPV infection and cervical cancer. This study was performed to compare the vaginal microbiota at two timepoints in women performing self-sampling and had a persistent or transient HPV16 infection. The women were tested for 12 high-risk HPV (hrHPV) types but only women with single type (HPV16) were included to reduce confounding variables. Methods In total 96 women were included in this study. Of these, 26 were single positive for HPV16 in the baseline test and HPV negative in the follow-up test and 38 were single positive for HPV16 in both tests and diagnosed with CIN2+ in histology. In addition, 32 women that were negative for all 12 HPV tested were included. The samples of vaginal fluid were analyzed with the Ion 16S™ Metagenomics Kit and Ion 16S™ metagenomics module within the Ion Reporter™ software. Results K-means clustering resulted in two Lactobacillus-dominated groups, one with Lactobacillus sp. and the other specifically with Lactobacillus iners. The two remaining clusters were dominated by a mixed non-Lactobacillus microbiota. HPV negative women had lower prevalence (28%) of the non-Lactobacill dominant cluster in the baseline test, as compared to women with HPV16 infection (42%) (p value = 0.0173). Transition between clusters were more frequent in women with persistent HPV16 infection (34%) as compared in women who cleared the HPV16 infection (19%) (p value = 0.036). Conclusions The vaginal microbiota showed a higher rate of transitioning between bacterial profiles in women with persistent HPV16 infection as compared to women with transient infection. This indicate an instability in the microenvironment in women with persistent HPV infection and development of CIN2+. |
| first_indexed | 2024-12-21T01:50:27Z |
| format | Article |
| id | doaj.art-fdcacec767ab420e847436c6306a79f3 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-21T01:50:27Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-fdcacec767ab420e847436c6306a79f32022-12-21T19:19:55ZengBMCVirology Journal1743-422X2020-10-011711910.1186/s12985-020-01420-zTemporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+Malin Berggrund0Inger Gustavsson1Riina Aarnio2Julia Hedlund Lindberg3Karin Sanner4Ingrid Wikström5Stefan Enroth6Ignas Bunikis7Matts Olovsson8Ulf Gyllensten9Department of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Uppsala UniversityDepartment of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Uppsala UniversityDepartment of Women’s and Children’s Health, Uppsala UniversityDepartment of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Uppsala UniversityDepartment of Women’s and Children’s Health, Uppsala UniversityDepartment of Women’s and Children’s Health, Uppsala UniversityDepartment of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Uppsala UniversityUppsala Genome Center, Science for Life Laboratory, Department of Immunology, Genetics, and Pathology, Uppsala UniversityDepartment of Women’s and Children’s Health, Uppsala UniversityDepartment of Immunology, Genetics, and Pathology, Biomedical Center, Science for Life Laboratory (SciLifeLab) Uppsala, Uppsala UniversityAbstract Background The vaginal microbiota has been reported to be associated with HPV infection and cervical cancer. This study was performed to compare the vaginal microbiota at two timepoints in women performing self-sampling and had a persistent or transient HPV16 infection. The women were tested for 12 high-risk HPV (hrHPV) types but only women with single type (HPV16) were included to reduce confounding variables. Methods In total 96 women were included in this study. Of these, 26 were single positive for HPV16 in the baseline test and HPV negative in the follow-up test and 38 were single positive for HPV16 in both tests and diagnosed with CIN2+ in histology. In addition, 32 women that were negative for all 12 HPV tested were included. The samples of vaginal fluid were analyzed with the Ion 16S™ Metagenomics Kit and Ion 16S™ metagenomics module within the Ion Reporter™ software. Results K-means clustering resulted in two Lactobacillus-dominated groups, one with Lactobacillus sp. and the other specifically with Lactobacillus iners. The two remaining clusters were dominated by a mixed non-Lactobacillus microbiota. HPV negative women had lower prevalence (28%) of the non-Lactobacill dominant cluster in the baseline test, as compared to women with HPV16 infection (42%) (p value = 0.0173). Transition between clusters were more frequent in women with persistent HPV16 infection (34%) as compared in women who cleared the HPV16 infection (19%) (p value = 0.036). Conclusions The vaginal microbiota showed a higher rate of transitioning between bacterial profiles in women with persistent HPV16 infection as compared to women with transient infection. This indicate an instability in the microenvironment in women with persistent HPV infection and development of CIN2+.http://link.springer.com/article/10.1186/s12985-020-01420-zVaginal microbiotaSelf-samplingHPVCIN2+Cervical cancerFTA card |
| spellingShingle | Malin Berggrund Inger Gustavsson Riina Aarnio Julia Hedlund Lindberg Karin Sanner Ingrid Wikström Stefan Enroth Ignas Bunikis Matts Olovsson Ulf Gyllensten Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ Virology Journal Vaginal microbiota Self-sampling HPV CIN2+ Cervical cancer FTA card |
| title | Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ |
| title_full | Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ |
| title_fullStr | Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ |
| title_full_unstemmed | Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ |
| title_short | Temporal changes in the vaginal microbiota in self-samples and its association with persistent HPV16 infection and CIN2+ |
| title_sort | temporal changes in the vaginal microbiota in self samples and its association with persistent hpv16 infection and cin2 |
| topic | Vaginal microbiota Self-sampling HPV CIN2+ Cervical cancer FTA card |
| url | http://link.springer.com/article/10.1186/s12985-020-01420-z |
| work_keys_str_mv | AT malinberggrund temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT ingergustavsson temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT riinaaarnio temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT juliahedlundlindberg temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT karinsanner temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT ingridwikstrom temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT stefanenroth temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT ignasbunikis temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT mattsolovsson temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 AT ulfgyllensten temporalchangesinthevaginalmicrobiotainselfsamplesanditsassociationwithpersistenthpv16infectionandcin2 |