Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells)
Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. <i>Ipomoea pes-caprae</i> (IPC) has been utilized for nutritional and therapeutic purposes,...
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MDPI AG
2022-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/8/969 |
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| author | Tasanee Panichakul Saranyoo Ponnikorn Wipa Tupchiangmai Woraphot Haritakun Kitima Srisanga |
| author_facet | Tasanee Panichakul Saranyoo Ponnikorn Wipa Tupchiangmai Woraphot Haritakun Kitima Srisanga |
| author_sort | Tasanee Panichakul |
| collection | DOAJ |
| description | Collagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. <i>Ipomoea pes-caprae</i> (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1A1), tumor growth factor beta 1 (TGFB1), and beta-fibroblast growth factor (FGF2) genes were also evaluated. The antioxidant and collagenase inhibitory properties of IPC extracts were associated with 3,5-di-caffeoylquinic acid, chlorogenic acid, and ferulic acid. IPC extracts at noncytotoxic concentrations significantly increased cell proliferation, collagen production, and wound healing. These effects appear linked to the upregulation of COL1A1, TGFB1, and FGF2 genes. The bioactivity of the IPC70 extract was greater than that for IPC95. This is useful in cosmeceutical applications for human skin aging. Our findings indicate that IPC extracts have the potential for use in skin anti-aging cosmeceutical preparations. |
| first_indexed | 2024-03-09T03:59:04Z |
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| last_indexed | 2024-03-09T03:59:04Z |
| publishDate | 2022-08-01 |
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| series | Pharmaceuticals |
| spelling | doaj.art-fdcaffc73a314875adcf922512d852f22023-12-03T14:16:17ZengMDPI AGPharmaceuticals1424-82472022-08-0115896910.3390/ph15080969Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells)Tasanee Panichakul0Saranyoo Ponnikorn1Wipa Tupchiangmai2Woraphot Haritakun3Kitima Srisanga4Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, 228-228/1-3 Sirindhorn Rd. Bangphlat, Bangkok 10700, ThailandChulabhorn International College of Medicine, Thammasat University (Rangsit Center), 99 Moo 18, Paholyothin Rd. Klongnung, Klongluang, Rangsit 12120, ThailandDepartment of Chemical Technology, Faculty of Science and Technology, Suan Dusit University, 228-228/1-3 Sirindhorn Rd. Bangphlat, Bangkok 10700, ThailandDepartment of Chemical Technology, Faculty of Science and Technology, Suan Dusit University, 228-228/1-3 Sirindhorn Rd. Bangphlat, Bangkok 10700, ThailandDepartment of Biochemistry, Faculty of Science, Mahidol University, 272 Rama VI Rd., Ratchathewi District, Bangkok 10400, ThailandCollagen loss in the skin dermis is a major cause of age-related changes to the skin. Natural phytochemical substances are desirable for the prevention of skin aging and the formation of wrinkles. <i>Ipomoea pes-caprae</i> (IPC) has been utilized for nutritional and therapeutic purposes, and its extract contains collagenase inhibitory activity while causing no cytotoxicity. The purpose of this study was to examine the impact of IPC extracts on cell proliferation and collagen production in human fibroblasts (CCD-986sk cells). IPC leaves were macerated in 70% and 95% ethanol and the chemical composition of the resulting extracts (IPC70 and IPC95) were determined using high performance liquid chromatography (HPLC). The bioactivity of IPC extracts was examined in CCD-986sk cells, including antioxidant capacity, inhibition of collagenase, effects on cell proliferation and collagen production, as well as wound healing using an in vitro scratch test. Changes in expression of collagen type I (COL1A1), tumor growth factor beta 1 (TGFB1), and beta-fibroblast growth factor (FGF2) genes were also evaluated. The antioxidant and collagenase inhibitory properties of IPC extracts were associated with 3,5-di-caffeoylquinic acid, chlorogenic acid, and ferulic acid. IPC extracts at noncytotoxic concentrations significantly increased cell proliferation, collagen production, and wound healing. These effects appear linked to the upregulation of COL1A1, TGFB1, and FGF2 genes. The bioactivity of the IPC70 extract was greater than that for IPC95. This is useful in cosmeceutical applications for human skin aging. Our findings indicate that IPC extracts have the potential for use in skin anti-aging cosmeceutical preparations.https://www.mdpi.com/1424-8247/15/8/969<i>Ipomoea pes-caprae</i>skin agingantioxidantcollagenasecollagencell proliferation |
| spellingShingle | Tasanee Panichakul Saranyoo Ponnikorn Wipa Tupchiangmai Woraphot Haritakun Kitima Srisanga Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) Pharmaceuticals <i>Ipomoea pes-caprae</i> skin aging antioxidant collagenase collagen cell proliferation |
| title | Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) |
| title_full | Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) |
| title_fullStr | Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) |
| title_full_unstemmed | Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) |
| title_short | Skin Anti-Aging Potential of <i>Ipomoea pes-caprae</i> Ethanolic Extracts on Promoting Cell Proliferation and Collagen Production in Human Fibroblasts (CCD-986sk Cells) |
| title_sort | skin anti aging potential of i ipomoea pes caprae i ethanolic extracts on promoting cell proliferation and collagen production in human fibroblasts ccd 986sk cells |
| topic | <i>Ipomoea pes-caprae</i> skin aging antioxidant collagenase collagen cell proliferation |
| url | https://www.mdpi.com/1424-8247/15/8/969 |
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