Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish
Streptococcus dysgalactiae is an emerging pathogen of fish. Clinically, infection is characterized by the development of necrotic lesions at the caudal peduncle of infected fishes. The pathogen has been recently isolated from different fish species in many countries. Twenty S. dysgalactiae isolates...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123213001471 |
| _version_ | 1818917712738385920 |
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| author | M. Abdelsalam A.E. Eissa S.-C. Chen |
| author_facet | M. Abdelsalam A.E. Eissa S.-C. Chen |
| author_sort | M. Abdelsalam |
| collection | DOAJ |
| description | Streptococcus dysgalactiae is an emerging pathogen of fish. Clinically, infection is characterized by the development of necrotic lesions at the caudal peduncle of infected fishes. The pathogen has been recently isolated from different fish species in many countries. Twenty S. dysgalactiae isolates collected from Japan, Taiwan, Malaysia and Indonesia were molecularly characterized by biased sinusoidal field gel electrophoresis (BSFGE) using SmaI enzyme, and tuf gene sequencing analysis. DNA sequencing of ten S. dysgalactiae revealed no genetic variation in the tuf amplicons, except for three strains. The restriction patterns of chromosomal DNA measured by BSFGE were differentiated into six distinct types and one subtype among collected strains. To our knowledge, this report gives the first snapshot of S. dysgalactiae isolates collected from different countries that are localized geographically and differed on a multinational level. This genetic unrelatedness among different isolates might suggest a high recombination rate and low genetic stability. |
| first_indexed | 2024-12-20T00:38:26Z |
| format | Article |
| id | doaj.art-fdcc7bcf1e4341bb90d7634842e42263 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 2090-1224 |
| language | English |
| last_indexed | 2024-12-20T00:38:26Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-fdcc7bcf1e4341bb90d7634842e422632022-12-21T19:59:41ZengElsevierJournal of Advanced Research2090-12322090-12242015-03-016223323810.1016/j.jare.2013.12.003Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fishM. Abdelsalam0A.E. Eissa1S.-C. Chen2Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Fish Diseases and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptGraduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung, TaiwanStreptococcus dysgalactiae is an emerging pathogen of fish. Clinically, infection is characterized by the development of necrotic lesions at the caudal peduncle of infected fishes. The pathogen has been recently isolated from different fish species in many countries. Twenty S. dysgalactiae isolates collected from Japan, Taiwan, Malaysia and Indonesia were molecularly characterized by biased sinusoidal field gel electrophoresis (BSFGE) using SmaI enzyme, and tuf gene sequencing analysis. DNA sequencing of ten S. dysgalactiae revealed no genetic variation in the tuf amplicons, except for three strains. The restriction patterns of chromosomal DNA measured by BSFGE were differentiated into six distinct types and one subtype among collected strains. To our knowledge, this report gives the first snapshot of S. dysgalactiae isolates collected from different countries that are localized geographically and differed on a multinational level. This genetic unrelatedness among different isolates might suggest a high recombination rate and low genetic stability.http://www.sciencedirect.com/science/article/pii/S2090123213001471Streptococcus dysgalactiaeEpidemiologytuf gene sequencingBSFGE |
| spellingShingle | M. Abdelsalam A.E. Eissa S.-C. Chen Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish Journal of Advanced Research Streptococcus dysgalactiae Epidemiology tuf gene sequencing BSFGE |
| title | Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish |
| title_full | Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish |
| title_fullStr | Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish |
| title_full_unstemmed | Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish |
| title_short | Genetic diversity of geographically distinct Streptococcus dysgalactiae isolates from fish |
| title_sort | genetic diversity of geographically distinct streptococcus dysgalactiae isolates from fish |
| topic | Streptococcus dysgalactiae Epidemiology tuf gene sequencing BSFGE |
| url | http://www.sciencedirect.com/science/article/pii/S2090123213001471 |
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