Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women
The risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2012-01-01
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| Series: | Acta Medica |
| Subjects: | |
| Online Access: | https://actamedica.lfhk.cuni.cz/55/3/0130/ |
| _version_ | 1811240562435555328 |
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| author | Danijela Vujošević Vineta Vuksanović Mario Poljak Nebojša Jokmanović |
| author_facet | Danijela Vujošević Vineta Vuksanović Mario Poljak Nebojša Jokmanović |
| author_sort | Danijela Vujošević |
| collection | DOAJ |
| description | The risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of HPV genotypes in studied group of women in Montenegro. HPV genotypes were determined using the method of enzyme restriction of PCR products amplified with group-specific primers MY09/MY11 and restricted with seven different restriction endonucleases. Out of the total number of women HPV infection was found in 1/5 of participants (20%). Genotyping performed in HPV DNA positive women shows that the HPV genotype 16 is dominant and present in more than 1/3 of the participants (36.8%). The second most frequent HPV infection is with HPV genotype 58 and it is found in 10.5% of participants. HPV 31 and HPV 6 infections are present in 7.9% of women, while infections with other genotypes were demonstrated individually by 2.6%. Multiple HPV infection was demonstrated in 18.4% and they are dominant in younger women (aged 25 to 30 years). |
| first_indexed | 2024-04-12T13:22:25Z |
| format | Article |
| id | doaj.art-fdcec2a980a44f58b6760f14471e0105 |
| institution | Directory Open Access Journal |
| issn | 1211-4286 1805-9694 |
| language | English |
| last_indexed | 2024-04-12T13:22:25Z |
| publishDate | 2012-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Acta Medica |
| spelling | doaj.art-fdcec2a980a44f58b6760f14471e01052022-12-22T03:31:26ZengKarolinum PressActa Medica1211-42861805-96942012-01-0155313013210.14712/18059694.2015.50Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin WomenDanijela Vujošević0Vineta Vuksanović1Mario Poljak2Nebojša Jokmanović3Center for Medical Microbiology, Institute of Public Health, Podgorica, MontenegroCenter for Medical Microbiology, Institute of Public Health, Podgorica, Montenegro; Medical Faculty, University of Montenegro, Podgorica, MontenegroInstitute of Microbiology and Immunology, Faculty of Medicine, Ljubljana, SloveniaClinic for Gynecology and Obstetrics, Clinical Center of Montenegro, Podgorica, MontenegroThe risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of HPV genotypes in studied group of women in Montenegro. HPV genotypes were determined using the method of enzyme restriction of PCR products amplified with group-specific primers MY09/MY11 and restricted with seven different restriction endonucleases. Out of the total number of women HPV infection was found in 1/5 of participants (20%). Genotyping performed in HPV DNA positive women shows that the HPV genotype 16 is dominant and present in more than 1/3 of the participants (36.8%). The second most frequent HPV infection is with HPV genotype 58 and it is found in 10.5% of participants. HPV 31 and HPV 6 infections are present in 7.9% of women, while infections with other genotypes were demonstrated individually by 2.6%. Multiple HPV infection was demonstrated in 18.4% and they are dominant in younger women (aged 25 to 30 years).https://actamedica.lfhk.cuni.cz/55/3/0130/HPV genotypePCRRFLPMultiple HPV infection |
| spellingShingle | Danijela Vujošević Vineta Vuksanović Mario Poljak Nebojša Jokmanović Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women Acta Medica HPV genotype PCR RFLP Multiple HPV infection |
| title | Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women |
| title_full | Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women |
| title_fullStr | Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women |
| title_full_unstemmed | Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women |
| title_short | Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women |
| title_sort | human papillomavirus genotype spectrum in studied group of montenegrin women |
| topic | HPV genotype PCR RFLP Multiple HPV infection |
| url | https://actamedica.lfhk.cuni.cz/55/3/0130/ |
| work_keys_str_mv | AT danijelavujosevic humanpapillomavirusgenotypespectruminstudiedgroupofmontenegrinwomen AT vinetavuksanovic humanpapillomavirusgenotypespectruminstudiedgroupofmontenegrinwomen AT mariopoljak humanpapillomavirusgenotypespectruminstudiedgroupofmontenegrinwomen AT nebojsajokmanovic humanpapillomavirusgenotypespectruminstudiedgroupofmontenegrinwomen |