Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification.
Loss of totipotentcy in an early embryo is directed by molecular processes responsible for cell fate decisions. Three dimensional genome organisation is an important factor linking chromatin architecture with stage specific gene expression patterns. Little is known about the role of chromosome organ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5540545?pdf=render |
| _version_ | 1818264572873670656 |
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| author | Maciej Orsztynowicz Dorota Lechniak Piotr Pawlak Beata Kociucka Svatava Kubickova Halina Cernohorska Zofia Eliza Madeja |
| author_facet | Maciej Orsztynowicz Dorota Lechniak Piotr Pawlak Beata Kociucka Svatava Kubickova Halina Cernohorska Zofia Eliza Madeja |
| author_sort | Maciej Orsztynowicz |
| collection | DOAJ |
| description | Loss of totipotentcy in an early embryo is directed by molecular processes responsible for cell fate decisions. Three dimensional genome organisation is an important factor linking chromatin architecture with stage specific gene expression patterns. Little is known about the role of chromosome organisation in gene expression regulation of lineage specific factors in mammalian embryos. Using bovine embryos as a model we have described these interactions at key developmental stages. Three bovine chromosomes (BTA) that differ in size, number of carried genes, and contain loci for key lineage regulators OCT4, NANOG and CDX2, were investigated. The results suggest that large chromosomes regardless of their gene density (BTA12 gene-poor, BTA5 gene-rich) do not significantly change their radial position within the nucleus. Gene loci however, may change its position within the chromosome territory (CT) and relocate its periphery, when stage specific process of gene activation is required. Trophectoderm specific CDX2 and epiblast precursor NANOG loci tend to locate on the surface or outside of the CTs, at stages related with their high expression. We postulate that the observed changes in CT shape reflect global alternations in gene expression related to differentiation. |
| first_indexed | 2024-12-12T19:37:03Z |
| format | Article |
| id | doaj.art-fdcfa136fa404ed69ee5ca905155f0d0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T19:37:03Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fdcfa136fa404ed69ee5ca905155f0d02022-12-22T00:14:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018239810.1371/journal.pone.0182398Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification.Maciej OrsztynowiczDorota LechniakPiotr PawlakBeata KociuckaSvatava KubickovaHalina CernohorskaZofia Eliza MadejaLoss of totipotentcy in an early embryo is directed by molecular processes responsible for cell fate decisions. Three dimensional genome organisation is an important factor linking chromatin architecture with stage specific gene expression patterns. Little is known about the role of chromosome organisation in gene expression regulation of lineage specific factors in mammalian embryos. Using bovine embryos as a model we have described these interactions at key developmental stages. Three bovine chromosomes (BTA) that differ in size, number of carried genes, and contain loci for key lineage regulators OCT4, NANOG and CDX2, were investigated. The results suggest that large chromosomes regardless of their gene density (BTA12 gene-poor, BTA5 gene-rich) do not significantly change their radial position within the nucleus. Gene loci however, may change its position within the chromosome territory (CT) and relocate its periphery, when stage specific process of gene activation is required. Trophectoderm specific CDX2 and epiblast precursor NANOG loci tend to locate on the surface or outside of the CTs, at stages related with their high expression. We postulate that the observed changes in CT shape reflect global alternations in gene expression related to differentiation.http://europepmc.org/articles/PMC5540545?pdf=render |
| spellingShingle | Maciej Orsztynowicz Dorota Lechniak Piotr Pawlak Beata Kociucka Svatava Kubickova Halina Cernohorska Zofia Eliza Madeja Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. PLoS ONE |
| title | Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. |
| title_full | Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. |
| title_fullStr | Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. |
| title_full_unstemmed | Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. |
| title_short | Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. |
| title_sort | changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification |
| url | http://europepmc.org/articles/PMC5540545?pdf=render |
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