Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant
Heavy metal (HM) emissions have increased due to the impact of rising urbanization and anthropogenic activity, affecting different parts of the environment. The goal of this study is to investigate the combined effect of ZnO NPs and bacteria treatment on protein and gene expression profiles of rice...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/10/6/305 |
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| author | Nazneen Akhtar Sehresh Khan Muhammad Jamil Shafiq Ur Rehman Zia Ur Rehman Eui Shik Rha |
| author_facet | Nazneen Akhtar Sehresh Khan Muhammad Jamil Shafiq Ur Rehman Zia Ur Rehman Eui Shik Rha |
| author_sort | Nazneen Akhtar |
| collection | DOAJ |
| description | Heavy metal (HM) emissions have increased due to the impact of rising urbanization and anthropogenic activity, affecting different parts of the environment. The goal of this study is to investigate the combined effect of ZnO NPs and bacteria treatment on protein and gene expression profiles of rice plants that are grown in HMs-polluted water. Seeds were primed with <i>Bacillus</i> spp. (<i>Bacillus cereus</i> and <i>Lysinibacillus macroides</i>) before being cultured in Hoagland media containing ZnO NPs (5 and 10 mg/L) and HMs-contaminated water from the Hayatabad industrial estate (HIE), Peshawar, Pakistan. The results revealed that the maximum nitrogen and protein content was observed in the root, shoot, and leaf of the plant grown by combining bacteria-ZnO NPs treatment under HMs stress as compared with plant grown without or with individual treatments of ZnO NPs and bacteria. Furthermore, protein expression analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) revealed that plants that were grown in HMs-polluted water were found to be affected in contaminated water, however the combined effect of bacteria-ZnO NPs reported the more dense protein profile as compared with their individual treatments. Subsequently, plants that were grown in HMs-polluted water have the highest expression levels of stress-induced genes such as myeloblastosis (<i>Myb</i>), zinc-finger protein (<i>Zat</i>-12), and ascorbate peroxidase (<i>Apx</i>) while the combined effect revealed minimum expression as compared with individual treatments. It is concluded that the combined effect of ZnO NPs and bacteria lowered the stress-induced gene expression while it increased the nitrogen-protein content and protein expression in plant grown under HMs stress. |
| first_indexed | 2024-03-09T22:20:45Z |
| format | Article |
| id | doaj.art-fdcfe8571dce4f39bb23c1511614a210 |
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| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T22:20:45Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-fdcfe8571dce4f39bb23c1511614a2102023-11-23T19:16:02ZengMDPI AGToxics2305-63042022-06-0110630510.3390/toxics10060305Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) PlantNazneen Akhtar0Sehresh Khan1Muhammad Jamil2Shafiq Ur Rehman3Zia Ur Rehman4Eui Shik Rha5Department of Biotechnology and Genetic Engineering, Kohat University of Science & Technology (KUST), Kohat 26000, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science & Technology (KUST), Kohat 26000, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science & Technology (KUST), Kohat 26000, PakistanDepartment of Biology, University of Haripur, Haripur 22620, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science & Technology (KUST), Kohat 26000, PakistanDepartment of Well-Being Resources, Sunchon National University, Suncheon 540-742, KoreaHeavy metal (HM) emissions have increased due to the impact of rising urbanization and anthropogenic activity, affecting different parts of the environment. The goal of this study is to investigate the combined effect of ZnO NPs and bacteria treatment on protein and gene expression profiles of rice plants that are grown in HMs-polluted water. Seeds were primed with <i>Bacillus</i> spp. (<i>Bacillus cereus</i> and <i>Lysinibacillus macroides</i>) before being cultured in Hoagland media containing ZnO NPs (5 and 10 mg/L) and HMs-contaminated water from the Hayatabad industrial estate (HIE), Peshawar, Pakistan. The results revealed that the maximum nitrogen and protein content was observed in the root, shoot, and leaf of the plant grown by combining bacteria-ZnO NPs treatment under HMs stress as compared with plant grown without or with individual treatments of ZnO NPs and bacteria. Furthermore, protein expression analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) revealed that plants that were grown in HMs-polluted water were found to be affected in contaminated water, however the combined effect of bacteria-ZnO NPs reported the more dense protein profile as compared with their individual treatments. Subsequently, plants that were grown in HMs-polluted water have the highest expression levels of stress-induced genes such as myeloblastosis (<i>Myb</i>), zinc-finger protein (<i>Zat</i>-12), and ascorbate peroxidase (<i>Apx</i>) while the combined effect revealed minimum expression as compared with individual treatments. It is concluded that the combined effect of ZnO NPs and bacteria lowered the stress-induced gene expression while it increased the nitrogen-protein content and protein expression in plant grown under HMs stress.https://www.mdpi.com/2305-6304/10/6/305heavy metalsnanoparticlestranscription factorsgel electrophoresiszinc oxide nanoparticlesbacteria |
| spellingShingle | Nazneen Akhtar Sehresh Khan Muhammad Jamil Shafiq Ur Rehman Zia Ur Rehman Eui Shik Rha Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant Toxics heavy metals nanoparticles transcription factors gel electrophoresis zinc oxide nanoparticles bacteria |
| title | Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant |
| title_full | Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant |
| title_fullStr | Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant |
| title_full_unstemmed | Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant |
| title_short | Combine Effect of ZnO NPs and Bacteria on Protein and Gene’s Expression Profile of Rice (<i>Oryza sativa</i> L.) Plant |
| title_sort | combine effect of zno nps and bacteria on protein and gene s expression profile of rice i oryza sativa i l plant |
| topic | heavy metals nanoparticles transcription factors gel electrophoresis zinc oxide nanoparticles bacteria |
| url | https://www.mdpi.com/2305-6304/10/6/305 |
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