Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil.
Viruses may represent the most diverse microorganisms on Earth. Novel viruses and variants continue to emerge. Mosquitoes are the most dangerous animals to humankind. This study aimed at identifying viral RNA diversity in salivary glands of mosquitoes captured in a sylvatic area of Cerrado at the Ch...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5678729?pdf=render |
| _version_ | 1819032236699156480 |
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| author | Andressa Zelenski de Lara Pinto Michellen Santos de Carvalho Fernando Lucas de Melo Ana Lúcia Maria Ribeiro Bergmann Morais Ribeiro Renata Dezengrini Slhessarenko |
| author_facet | Andressa Zelenski de Lara Pinto Michellen Santos de Carvalho Fernando Lucas de Melo Ana Lúcia Maria Ribeiro Bergmann Morais Ribeiro Renata Dezengrini Slhessarenko |
| author_sort | Andressa Zelenski de Lara Pinto |
| collection | DOAJ |
| description | Viruses may represent the most diverse microorganisms on Earth. Novel viruses and variants continue to emerge. Mosquitoes are the most dangerous animals to humankind. This study aimed at identifying viral RNA diversity in salivary glands of mosquitoes captured in a sylvatic area of Cerrado at the Chapada dos Guimarães National Park, Mato Grosso, Brazil. In total, 66 Culicinae mosquitoes belonging to 16 species comprised 9 pools, subjected to viral RNA extraction, double-strand cDNA synthesis, random amplification and high-throughput sequencing, revealing the presence of seven insect-specific viruses, six of which represent new species of Rhabdoviridae (Lobeira virus), Chuviridae (Cumbaru and Croada viruses), Totiviridae (Murici virus) and Partitiviridae (Araticum and Angico viruses). In addition, two mosquito pools presented Kaiowa virus sequences that had already been reported in South Pantanal, Brazil. These findings amplify the understanding of viral diversity in wild-type Culicinae. Insect-specific viruses may present a broader diversity than previously imagined and future studies may address their possible role in mosquito vector competence. |
| first_indexed | 2024-12-21T06:58:44Z |
| format | Article |
| id | doaj.art-fdd1a52c165d42f1ada743ebbbe92432 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T06:58:44Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fdd1a52c165d42f1ada743ebbbe924322022-12-21T19:12:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018742910.1371/journal.pone.0187429Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil.Andressa Zelenski de Lara PintoMichellen Santos de CarvalhoFernando Lucas de MeloAna Lúcia Maria RibeiroBergmann Morais RibeiroRenata Dezengrini SlhessarenkoViruses may represent the most diverse microorganisms on Earth. Novel viruses and variants continue to emerge. Mosquitoes are the most dangerous animals to humankind. This study aimed at identifying viral RNA diversity in salivary glands of mosquitoes captured in a sylvatic area of Cerrado at the Chapada dos Guimarães National Park, Mato Grosso, Brazil. In total, 66 Culicinae mosquitoes belonging to 16 species comprised 9 pools, subjected to viral RNA extraction, double-strand cDNA synthesis, random amplification and high-throughput sequencing, revealing the presence of seven insect-specific viruses, six of which represent new species of Rhabdoviridae (Lobeira virus), Chuviridae (Cumbaru and Croada viruses), Totiviridae (Murici virus) and Partitiviridae (Araticum and Angico viruses). In addition, two mosquito pools presented Kaiowa virus sequences that had already been reported in South Pantanal, Brazil. These findings amplify the understanding of viral diversity in wild-type Culicinae. Insect-specific viruses may present a broader diversity than previously imagined and future studies may address their possible role in mosquito vector competence.http://europepmc.org/articles/PMC5678729?pdf=render |
| spellingShingle | Andressa Zelenski de Lara Pinto Michellen Santos de Carvalho Fernando Lucas de Melo Ana Lúcia Maria Ribeiro Bergmann Morais Ribeiro Renata Dezengrini Slhessarenko Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. PLoS ONE |
| title | Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. |
| title_full | Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. |
| title_fullStr | Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. |
| title_full_unstemmed | Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. |
| title_short | Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil. |
| title_sort | novel viruses in salivary glands of mosquitoes from sylvatic cerrado midwestern brazil |
| url | http://europepmc.org/articles/PMC5678729?pdf=render |
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