Robust T cell activation requires an eIF3-driven burst in T cell receptor translation

Robust T cell activation requires an eIF3-driven burst in T cell receptor translation

Activation of T cells requires a rapid surge in cellular protein synthesis. However, the role of translation initiation in the early induction of specific genes remains unclear. Here, we show human translation initiation factor eIF3 interacts with select immune system related mRNAs including those e...

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Bibliographic Details
Main Authors: Dasmanthie De Silva, Lucas Ferguson, Grant H Chin, Benjamin E Smith, Ryan A Apathy, Theodore L Roth, Franziska Blaeschke, Marek Kudla, Alexander Marson, Nicholas T Ingolia, Jamie HD Cate
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-12-01
Series:eLife
Subjects:
eIF3
protein synthesis
T cell receptor
CD28
chimeric antigen receptor
cellular immunotherapy
Online Access:https://elifesciences.org/articles/74272
Description
Summary:Activation of T cells requires a rapid surge in cellular protein synthesis. However, the role of translation initiation in the early induction of specific genes remains unclear. Here, we show human translation initiation factor eIF3 interacts with select immune system related mRNAs including those encoding the T cell receptor (TCR) subunits TCRA and TCRB. Binding of eIF3 to the TCRA and TCRB mRNA 3’-untranslated regions (3’-UTRs) depends on CD28 coreceptor signaling and regulates a burst in TCR translation required for robust T cell activation. Use of the TCRA or TCRB 3’-UTRs to control expression of an anti-CD19 chimeric antigen receptor (CAR) improves the ability of CAR-T cells to kill tumor cells in vitro. These results identify a new mechanism of eIF3-mediated translation control that can aid T cell engineering for immunotherapy applications.
ISSN:2050-084X

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