Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields
Abstract In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) le...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2021-06-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=en |
| _version_ | 1798015551496257536 |
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| author | LARISA ILIJIN MARIJA MRDAKOVIĆ DAJANA TODOROVIĆ MILENA VLAHOVIĆ ANJA GRČIĆ ALEKSANDRA FILIPOVIĆ VESNA PERIĆ-MATARUGA |
| author_facet | LARISA ILIJIN MARIJA MRDAKOVIĆ DAJANA TODOROVIĆ MILENA VLAHOVIĆ ANJA GRČIĆ ALEKSANDRA FILIPOVIĆ VESNA PERIĆ-MATARUGA |
| author_sort | LARISA ILIJIN |
| collection | DOAJ |
| description | Abstract In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch – off in the expression of constitutive and/or inducible HSP70 isoforms. |
| first_indexed | 2024-04-11T15:36:26Z |
| format | Article |
| id | doaj.art-fdd70162acad402aa660dfbf939b474d |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-11T15:36:26Z |
| publishDate | 2021-06-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-fdd70162acad402aa660dfbf939b474d2022-12-22T04:15:57ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-06-0193210.1590/0001-3765202120190118Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fieldsLARISA ILIJINhttps://orcid.org/0000-0003-1728-6140MARIJA MRDAKOVIĆhttps://orcid.org/0000-0003-3946-5828DAJANA TODOROVIĆhttps://orcid.org/0000-0003-1796-9312MILENA VLAHOVIĆhttps://orcid.org/0000-0002-7815-091XANJA GRČIĆhttps://orcid.org/0000-0001-9306-0691ALEKSANDRA FILIPOVIĆhttps://orcid.org/0000-0003-3578-5672VESNA PERIĆ-MATARUGAhttps://orcid.org/0000-0002-2899-7418Abstract In this paper, we analyzed the effects of chronic exposure (5 months) to static magnetic field (110 mT; SMF) and extremely low frequency magnetic field (ELF MF; 10 mT, 50 Hz) on Blaptica dubia nymphs. We have examined acetylcholinesterase (AChE) activity and heat shock protein 70 (HSP70) level, two sensitive biomarkers of stress in terrestrial insects. Relative growth rate (RGR), as a life history trait, was estimated. AChE activity was determined spectrophotometrically and HSP70 levels were quantified using indirect non-competitive ELISA and Western blotting. Calculated RGR was significantly changed upon exposure to both types of ambiental MFs. The effects of chronic exposure of B. dubia nymphs to SMF and ELF MF (50 Hz) were observed as decreased activity of AChE. The increased level of HSP70 was present only after exposure to SMF. The strength of ELF MF was most likely below the energy level needed to induce the expression of this stress protein. Different patterns of the expression of two HSP70 isoforms, where isoform 2 was sensitive only to SMF, are most likely a possibly switch – off in the expression of constitutive and/or inducible HSP70 isoforms.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=enAcetylcholinesteraseHSP70 isoformsmagnetic fieldrelative growth rate |
| spellingShingle | LARISA ILIJIN MARIJA MRDAKOVIĆ DAJANA TODOROVIĆ MILENA VLAHOVIĆ ANJA GRČIĆ ALEKSANDRA FILIPOVIĆ VESNA PERIĆ-MATARUGA Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields Anais da Academia Brasileira de Ciências Acetylcholinesterase HSP70 isoforms magnetic field relative growth rate |
| title | Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields |
| title_full | Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields |
| title_fullStr | Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields |
| title_full_unstemmed | Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields |
| title_short | Biological effects of chronic exposure of Blaptica dubia (Blattodea: Blaberidae) nymphs to static and extremely low frequency magnetic fields |
| title_sort | biological effects of chronic exposure of blaptica dubia blattodea blaberidae nymphs to static and extremely low frequency magnetic fields |
| topic | Acetylcholinesterase HSP70 isoforms magnetic field relative growth rate |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300811&tlng=en |
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