Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa
Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobact...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Veterinary World
2021-11-01
|
| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.14/November-2021/12.pdf |
| _version_ | 1818652123577974784 |
|---|---|
| author | Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima |
| author_facet | Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima |
| author_sort | Kealeboga Mileng |
| collection | DOAJ |
| description | Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobacter species in chickens from North West Province of South Africa as well as their antibiotic sensitivity status.
Materials and Methods: A total of 2400 chicken fecal samples were collected and pooled to a total of 480 samples from five registered active poultry abattoirs in the Ngaka Modiri Molema District of North West Province, South Africa. Polymerase chain reaction (PCR) was used for the detection of Campylobacter spp. targeting the 16S rRNA gene while antibiotic sensitivity was determined using disk diffusion inhibition test.
Results: After isolation, a total of 26 samples were confirmed to be harboring Campylobacter jejuni by PCR and sequencing. C. jejuni was found to be the only isolate detected in all the fecal samples tested. The study further demonstrated that C. jejuni infections were highest in the summer season (3%) followed by autumn and winter at 1%, while there were none detected in the spring. The isolated C. jejuni-positive samples on disk diffusion inhibition test displayed resistance to nalidixic acid, tetracycline, erythromycin, and ciprofloxacin at 98%, 80%, 83%, and 21%, respectively.
Conclusion: C. jejuni isolated in this study is known to cause disease in humans, and thus its occurrence requires application of "One Health" strategy to reduce the spread of this zoonotic pathogen in South Africa. |
| first_indexed | 2024-12-17T02:17:00Z |
| format | Article |
| id | doaj.art-fdd718166d684c2eabd1352b012d873c |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T02:17:00Z |
| publishDate | 2021-11-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-fdd718166d684c2eabd1352b012d873c2022-12-21T22:07:23ZengVeterinary WorldVeterinary World0972-89882231-09162021-11-0114112929293510.14202/vetworld.2021.2929-2935Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South AfricaKealeboga Mileng0https://orcid.org/0000-0002-7052-7209Tsepo A. Ramatla1https://orcid.org/0000-0002-0473-8075Rendani V. Ndou2https://orcid.org/0000-0002-4894-2246Oriel M. M. Thekisoe3https://orcid.org/0000-0003-0700-4787Michelo Syakalima4https://orcid.org/0000-0002-0649-5409Department of Animal Health, School of Agriculture, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.Department of Animal Health, School of Agriculture, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa; Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2531, South Africa.Department of Animal Health, School of Agriculture, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom 2531, South Africa.Department of Animal Health, School of Agriculture, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.Background and Aim: Infections with Campylobacter species have gained recognition as the most frequent cause of foodborne gastroenteritis globally. Their significance in South Africa is still an area of study interest. This study was, therefore, carried out to determine the occurrence of Campylobacter species in chickens from North West Province of South Africa as well as their antibiotic sensitivity status. Materials and Methods: A total of 2400 chicken fecal samples were collected and pooled to a total of 480 samples from five registered active poultry abattoirs in the Ngaka Modiri Molema District of North West Province, South Africa. Polymerase chain reaction (PCR) was used for the detection of Campylobacter spp. targeting the 16S rRNA gene while antibiotic sensitivity was determined using disk diffusion inhibition test. Results: After isolation, a total of 26 samples were confirmed to be harboring Campylobacter jejuni by PCR and sequencing. C. jejuni was found to be the only isolate detected in all the fecal samples tested. The study further demonstrated that C. jejuni infections were highest in the summer season (3%) followed by autumn and winter at 1%, while there were none detected in the spring. The isolated C. jejuni-positive samples on disk diffusion inhibition test displayed resistance to nalidixic acid, tetracycline, erythromycin, and ciprofloxacin at 98%, 80%, 83%, and 21%, respectively. Conclusion: C. jejuni isolated in this study is known to cause disease in humans, and thus its occurrence requires application of "One Health" strategy to reduce the spread of this zoonotic pathogen in South Africa.http://www.veterinaryworld.org/Vol.14/November-2021/12.pdfantibiotic resistancecampylobacter jejunichickenssouth africa |
| spellingShingle | Kealeboga Mileng Tsepo A. Ramatla Rendani V. Ndou Oriel M. M. Thekisoe Michelo Syakalima Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa Veterinary World antibiotic resistance campylobacter jejuni chickens south africa |
| title | Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa |
| title_full | Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa |
| title_fullStr | Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa |
| title_full_unstemmed | Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa |
| title_short | Isolation and antibiotic sensitivity of Campylobacter species from fecal samples of broiler chickens in North West Province, South Africa |
| title_sort | isolation and antibiotic sensitivity of campylobacter species from fecal samples of broiler chickens in north west province south africa |
| topic | antibiotic resistance campylobacter jejuni chickens south africa |
| url | http://www.veterinaryworld.org/Vol.14/November-2021/12.pdf |
| work_keys_str_mv | AT kealebogamileng isolationandantibioticsensitivityofcampylobacterspeciesfromfecalsamplesofbroilerchickensinnorthwestprovincesouthafrica AT tsepoaramatla isolationandantibioticsensitivityofcampylobacterspeciesfromfecalsamplesofbroilerchickensinnorthwestprovincesouthafrica AT rendanivndou isolationandantibioticsensitivityofcampylobacterspeciesfromfecalsamplesofbroilerchickensinnorthwestprovincesouthafrica AT orielmmthekisoe isolationandantibioticsensitivityofcampylobacterspeciesfromfecalsamplesofbroilerchickensinnorthwestprovincesouthafrica AT michelosyakalima isolationandantibioticsensitivityofcampylobacterspeciesfromfecalsamplesofbroilerchickensinnorthwestprovincesouthafrica |