Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma
<p>Abstract</p> <p>Background</p> <p>This study evaluates the existence of numerical alterations of chromosome 17 and <it>TP53 </it>gene deletion in gastric adenocarcinoma. The p53 protein expression was also evaluated, as well as, possible associations with...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-07-01
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| Series: | BMC Gastroenterology |
| Online Access: | http://www.biomedcentral.com/1471-230X/9/55 |
| _version_ | 1819086209772683264 |
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| author | Demachki Samia Assumpção Paulo P Rabenhorst Silvia HB Faria Mário HG Leal Mariana F Lima Eleonidas M Seabra Aline D Calcagno Danielle Q Guimarães Adriana C Khayat André S Smith Marília AC Burbano Rommel R |
| author_facet | Demachki Samia Assumpção Paulo P Rabenhorst Silvia HB Faria Mário HG Leal Mariana F Lima Eleonidas M Seabra Aline D Calcagno Danielle Q Guimarães Adriana C Khayat André S Smith Marília AC Burbano Rommel R |
| author_sort | Demachki Samia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>This study evaluates the existence of numerical alterations of chromosome 17 and <it>TP53 </it>gene deletion in gastric adenocarcinoma. The p53 protein expression was also evaluated, as well as, possible associations with clinicopathological characteristics.</p> <p>Methods</p> <p>Dual-color fluorescence <it>in situ </it>hybridization and immunostaining were performed in twenty gastric cancer samples of individuals from Northern Brazil.</p> <p>Results</p> <p>Deletion of <it>TP53 </it>was found in all samples. <it>TP53 </it>was inactivated mainly by single allelic deletion, varying to 7–39% of cells/case. Aneusomy of chromosome 17 was observed in 85% of cases. Chromosome 17 monosomy and gain were both observed in about half of cases. Cells with gain of chromosome 17 frequently presented <it>TP53 </it>deletion. The frequency of cells with two chr17 and one <it>TP53 </it>signals observed was higher in diffuse than in intestinal-type GC. Immunoreactivity of p53 was found only in intestinal-type samples. The frequency of cells with two chr17 and two <it>TP53 </it>signals found was higher in samples with positive p53 expression than in negative cases in intestinal-type GC.</p> <p>Conclusion</p> <p>We suggest that <it>TP53 </it>deletion and chromosome 17 aneusomy is a common event in GC and other <it>TP53 </it>alterations, as mutation, may be implicated in the distinct carcinogenesis process of diffuse and intestinal types.</p> |
| first_indexed | 2024-12-21T21:16:37Z |
| format | Article |
| id | doaj.art-fdd92d23239e462cb61d924113d80377 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-21T21:16:37Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-fdd92d23239e462cb61d924113d803772022-12-21T18:49:59ZengBMCBMC Gastroenterology1471-230X2009-07-01915510.1186/1471-230X-9-55Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinomaDemachki SamiaAssumpção Paulo PRabenhorst Silvia HBFaria Mário HGLeal Mariana FLima Eleonidas MSeabra Aline DCalcagno Danielle QGuimarães Adriana CKhayat André SSmith Marília ACBurbano Rommel R<p>Abstract</p> <p>Background</p> <p>This study evaluates the existence of numerical alterations of chromosome 17 and <it>TP53 </it>gene deletion in gastric adenocarcinoma. The p53 protein expression was also evaluated, as well as, possible associations with clinicopathological characteristics.</p> <p>Methods</p> <p>Dual-color fluorescence <it>in situ </it>hybridization and immunostaining were performed in twenty gastric cancer samples of individuals from Northern Brazil.</p> <p>Results</p> <p>Deletion of <it>TP53 </it>was found in all samples. <it>TP53 </it>was inactivated mainly by single allelic deletion, varying to 7–39% of cells/case. Aneusomy of chromosome 17 was observed in 85% of cases. Chromosome 17 monosomy and gain were both observed in about half of cases. Cells with gain of chromosome 17 frequently presented <it>TP53 </it>deletion. The frequency of cells with two chr17 and one <it>TP53 </it>signals observed was higher in diffuse than in intestinal-type GC. Immunoreactivity of p53 was found only in intestinal-type samples. The frequency of cells with two chr17 and two <it>TP53 </it>signals found was higher in samples with positive p53 expression than in negative cases in intestinal-type GC.</p> <p>Conclusion</p> <p>We suggest that <it>TP53 </it>deletion and chromosome 17 aneusomy is a common event in GC and other <it>TP53 </it>alterations, as mutation, may be implicated in the distinct carcinogenesis process of diffuse and intestinal types.</p>http://www.biomedcentral.com/1471-230X/9/55 |
| spellingShingle | Demachki Samia Assumpção Paulo P Rabenhorst Silvia HB Faria Mário HG Leal Mariana F Lima Eleonidas M Seabra Aline D Calcagno Danielle Q Guimarães Adriana C Khayat André S Smith Marília AC Burbano Rommel R Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma BMC Gastroenterology |
| title | Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| title_full | Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| title_fullStr | Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| title_full_unstemmed | Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| title_short | Interrelationship between <it>TP53 </it>gene deletion, protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| title_sort | interrelationship between it tp53 it gene deletion protein expression and chromosome 17 aneusomy in gastric adenocarcinoma |
| url | http://www.biomedcentral.com/1471-230X/9/55 |
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