Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences
Three species of flatworms from the genus Echinococcus (E. granulosus, E. multilocularis and E. vogeli) and four strains of E. granulosus (cattle, horse, pig and sheep strains) were analysed by the PCR-SSCP method followed by sequencing, using as targets two non-coding and two coding (one nuclear an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1998-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500024 |
| _version_ | 1797703012814159872 |
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| author | KL Haag AM Araújo B Gottstein A Zaha |
| author_facet | KL Haag AM Araújo B Gottstein A Zaha |
| author_sort | KL Haag |
| collection | DOAJ |
| description | Three species of flatworms from the genus Echinococcus (E. granulosus, E. multilocularis and E. vogeli) and four strains of E. granulosus (cattle, horse, pig and sheep strains) were analysed by the PCR-SSCP method followed by sequencing, using as targets two non-coding and two coding (one nuclear and one mitochondrial) genomic regions. The sequencing data was used to evaluate hypothesis about the parasite breeding system and the causes of genetic diversification. The calculated recombination parameters suggested that cross-fertilisation was rare in the history of the group. However, the relative rates of substitution in the coding sequences showed that positive selection (instead of purifying selection) drove the evolution of an elastase and neutrophil chemotaxis inhibitor gene (AgB/1). The phylogenetic analyses revealed several ambiguities, indicating that the taxonomic status of the E. granulosus horse strain should be revised |
| first_indexed | 2024-03-12T04:58:17Z |
| format | Article |
| id | doaj.art-fdda1d3a72104acb93acecfb233dcde6 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:17Z |
| publishDate | 1998-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-fdda1d3a72104acb93acecfb233dcde62023-09-03T09:14:40ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601998-09-0193569510.1590/S0074-02761998000500024Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide SequencesKL HaagAM AraújoB GottsteinA ZahaThree species of flatworms from the genus Echinococcus (E. granulosus, E. multilocularis and E. vogeli) and four strains of E. granulosus (cattle, horse, pig and sheep strains) were analysed by the PCR-SSCP method followed by sequencing, using as targets two non-coding and two coding (one nuclear and one mitochondrial) genomic regions. The sequencing data was used to evaluate hypothesis about the parasite breeding system and the causes of genetic diversification. The calculated recombination parameters suggested that cross-fertilisation was rare in the history of the group. However, the relative rates of substitution in the coding sequences showed that positive selection (instead of purifying selection) drove the evolution of an elastase and neutrophil chemotaxis inhibitor gene (AgB/1). The phylogenetic analyses revealed several ambiguities, indicating that the taxonomic status of the E. granulosus horse strain should be revisedhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500024EchinococcusparasitesrecombinationSSCPsequencingphylogeny |
| spellingShingle | KL Haag AM Araújo B Gottstein A Zaha Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences Memorias do Instituto Oswaldo Cruz Echinococcus parasites recombination SSCP sequencing phylogeny |
| title | Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences |
| title_full | Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences |
| title_fullStr | Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences |
| title_full_unstemmed | Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences |
| title_short | Selection, Recombination and History in a Parasitic Flatworm (Echinococcus) Inferred from Nucleotide Sequences |
| title_sort | selection recombination and history in a parasitic flatworm echinococcus inferred from nucleotide sequences |
| topic | Echinococcus parasites recombination SSCP sequencing phylogeny |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000500024 |
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