Jejunoileal mucosal growth in mice with a limited microbiome.
Previous work demonstrated enhanced enterocyte proliferation and mucosal growth in gnotobiotic mice, suggesting that intestinal flora participate in mucosal homeostasis. Furthermore, broad-spectrum enteral antibiotics are known to induce near germ-free (GF) conditions in mice with conventional flora...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0266251 |
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| author | Matthew P Shaughnessy Christine J Park Pooja S Salvi Robert A Cowles |
| author_facet | Matthew P Shaughnessy Christine J Park Pooja S Salvi Robert A Cowles |
| author_sort | Matthew P Shaughnessy |
| collection | DOAJ |
| description | Previous work demonstrated enhanced enterocyte proliferation and mucosal growth in gnotobiotic mice, suggesting that intestinal flora participate in mucosal homeostasis. Furthermore, broad-spectrum enteral antibiotics are known to induce near germ-free (GF) conditions in mice with conventional flora (CONV). We hypothesized that inducing near GF conditions with broad-spectrum enteral antibiotics would cause ordered small intestinal mucosal growth in CONV mice but would have no effect in GF mice with no inherent microbiome. C57BL/6J CONV and GF mice received either an antibiotic solution (Ampicillin, Ciprofloxacin, Metronidazole, Vancomycin, Meropenem) or a vehicle alone. After treatment, small intestinal villus height (VH), crypt depth (CD), mucosal surface area (MSA), crypt proliferation index (CPI), apoptosis, and villus and crypt cell types were assessed. Antibiotic-treated CONV (Abx-CONV) mice had taller villi, deeper crypts, increased CPI, increased apoptosis, and greater MSA compared to vehicle-treated CONV mice. Minor differences were noted in enterocyte and enterochromaffin cell proportions between groups, but goblet and Paneth cell proportions were unchanged in Abx-CONV mice compared to vehicle-treated CONV mice (p>0.05). Antibiotics caused no significant changes in VH or MSA in GF mice when compared to vehicle-treated GF mice (p>0.05). Enteral administration of broad-spectrum antibiotics to mice with a conventional microbiome stimulates ordered small intestinal mucosal growth. Mucosal growth was not seen in germ-free mice treated with antibiotics, implying that intestinal mucosal growth is associated with change in the microbiome in this model. |
| first_indexed | 2024-04-13T08:35:09Z |
| format | Article |
| id | doaj.art-fdddd7a8f72e4b5f9185cb41a7c5cb40 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T08:35:09Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fdddd7a8f72e4b5f9185cb41a7c5cb402022-12-22T02:54:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026625110.1371/journal.pone.0266251Jejunoileal mucosal growth in mice with a limited microbiome.Matthew P ShaughnessyChristine J ParkPooja S SalviRobert A CowlesPrevious work demonstrated enhanced enterocyte proliferation and mucosal growth in gnotobiotic mice, suggesting that intestinal flora participate in mucosal homeostasis. Furthermore, broad-spectrum enteral antibiotics are known to induce near germ-free (GF) conditions in mice with conventional flora (CONV). We hypothesized that inducing near GF conditions with broad-spectrum enteral antibiotics would cause ordered small intestinal mucosal growth in CONV mice but would have no effect in GF mice with no inherent microbiome. C57BL/6J CONV and GF mice received either an antibiotic solution (Ampicillin, Ciprofloxacin, Metronidazole, Vancomycin, Meropenem) or a vehicle alone. After treatment, small intestinal villus height (VH), crypt depth (CD), mucosal surface area (MSA), crypt proliferation index (CPI), apoptosis, and villus and crypt cell types were assessed. Antibiotic-treated CONV (Abx-CONV) mice had taller villi, deeper crypts, increased CPI, increased apoptosis, and greater MSA compared to vehicle-treated CONV mice. Minor differences were noted in enterocyte and enterochromaffin cell proportions between groups, but goblet and Paneth cell proportions were unchanged in Abx-CONV mice compared to vehicle-treated CONV mice (p>0.05). Antibiotics caused no significant changes in VH or MSA in GF mice when compared to vehicle-treated GF mice (p>0.05). Enteral administration of broad-spectrum antibiotics to mice with a conventional microbiome stimulates ordered small intestinal mucosal growth. Mucosal growth was not seen in germ-free mice treated with antibiotics, implying that intestinal mucosal growth is associated with change in the microbiome in this model.https://doi.org/10.1371/journal.pone.0266251 |
| spellingShingle | Matthew P Shaughnessy Christine J Park Pooja S Salvi Robert A Cowles Jejunoileal mucosal growth in mice with a limited microbiome. PLoS ONE |
| title | Jejunoileal mucosal growth in mice with a limited microbiome. |
| title_full | Jejunoileal mucosal growth in mice with a limited microbiome. |
| title_fullStr | Jejunoileal mucosal growth in mice with a limited microbiome. |
| title_full_unstemmed | Jejunoileal mucosal growth in mice with a limited microbiome. |
| title_short | Jejunoileal mucosal growth in mice with a limited microbiome. |
| title_sort | jejunoileal mucosal growth in mice with a limited microbiome |
| url | https://doi.org/10.1371/journal.pone.0266251 |
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