Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes
Background: Genotyping offers a promising avenue for identifying the healthy reproductive system in cows. The healthy reproductive system in cows is determined by measuring the level of ovulation and by identifying the type polymorphism of specific genes. Aim: The aim of the article is to explore...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2023-06-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=136165 |
| _version_ | 1827975725882277888 |
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| author | Yktiyar Sarybayev Yessengali Ussenbekov Orynbassar Turebekov Bekbolat Bekbolat Ibragim Tutkyshbay |
| author_facet | Yktiyar Sarybayev Yessengali Ussenbekov Orynbassar Turebekov Bekbolat Bekbolat Ibragim Tutkyshbay |
| author_sort | Yktiyar Sarybayev |
| collection | DOAJ |
| description | Background:
Genotyping offers a promising avenue for identifying the healthy reproductive system in cows. The healthy reproductive system in cows is determined by measuring the level of ovulation and by identifying the type polymorphism of specific genes.
Aim:
The aim of the article is to explore how polymorphism of Follicle Stimulating Hormone Receptor (FSHR) and Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) genes affect the reproduction trait of Holstein cows.
Methods:
Here we define a reproducible protocol to genotype and identify the polymorphism in specific genes from the extracted DNA of cows.
Results:
The results of genotyping showed that the only C allele (CC genotype) was observed in 100% of cows at the LHCGR locus, and three genotypes were observed at the FSHR locus (CC – 67.74%, CG - 9.03%, GG – 23.22%). In cows with the CC genotype at the FSHR locus, the hormone concentration during ovulation was 1.1 – 2.5 ng/ml, which is within the physiological range for healthy reproduction.
Conclusion:
Cows with the CC genotype at the FSHR locus have a healthy course of the ovulation process, therefore good reproduction. [Open Vet J 2023; 13(3.000): 352-357] |
| first_indexed | 2024-04-09T20:15:59Z |
| format | Article |
| id | doaj.art-fded58b99fb64dd18c42f750dc9503b9 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-04-09T20:15:59Z |
| publishDate | 2023-06-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-fded58b99fb64dd18c42f750dc9503b92023-03-31T10:18:09ZengTripoli UniversityOpen Veterinary Journal2226-44852023-06-0113335235710.5455/OVJ.2023.v13.i3.12136165Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genesYktiyar Sarybayev0Yessengali Ussenbekov1Orynbassar Turebekov2Bekbolat Bekbolat3Ibragim Tutkyshbay4Kazakh National Agrarian Research University Kazakh National Agrarian Research University Kazakh National Agrarian Research University Mukhtar Auezov South Kazakhstan University Mukhtar Auezov South Kazakhstan UniversityBackground: Genotyping offers a promising avenue for identifying the healthy reproductive system in cows. The healthy reproductive system in cows is determined by measuring the level of ovulation and by identifying the type polymorphism of specific genes. Aim: The aim of the article is to explore how polymorphism of Follicle Stimulating Hormone Receptor (FSHR) and Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) genes affect the reproduction trait of Holstein cows. Methods: Here we define a reproducible protocol to genotype and identify the polymorphism in specific genes from the extracted DNA of cows. Results: The results of genotyping showed that the only C allele (CC genotype) was observed in 100% of cows at the LHCGR locus, and three genotypes were observed at the FSHR locus (CC – 67.74%, CG - 9.03%, GG – 23.22%). In cows with the CC genotype at the FSHR locus, the hormone concentration during ovulation was 1.1 – 2.5 ng/ml, which is within the physiological range for healthy reproduction. Conclusion: Cows with the CC genotype at the FSHR locus have a healthy course of the ovulation process, therefore good reproduction. [Open Vet J 2023; 13(3.000): 352-357]http://www.ejmanager.com/fulltextpdf.php?mno=136165genotypingovulationlhcgrfshrpolymorphism |
| spellingShingle | Yktiyar Sarybayev Yessengali Ussenbekov Orynbassar Turebekov Bekbolat Bekbolat Ibragim Tutkyshbay Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes Open Veterinary Journal genotyping ovulation lhcgr fshr polymorphism |
| title | Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes |
| title_full | Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes |
| title_fullStr | Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes |
| title_full_unstemmed | Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes |
| title_short | Genotyping of cows by LHCGR, FSHR loci and determination of the level of ovulation depending on the expression of the studied genes |
| title_sort | genotyping of cows by lhcgr fshr loci and determination of the level of ovulation depending on the expression of the studied genes |
| topic | genotyping ovulation lhcgr fshr polymorphism |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=136165 |
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