Novel Picornavirus in Turkey Poults with Hepatitis, California, USA
To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008–2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) reveale...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2011-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/17/3/10-1410_article |
| _version_ | 1828257711371845632 |
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| author | Kirsi S. Honkavuori H. L. Shivaprasad Thomas Briese Craig Street David L. Hirschberg Stephen K. Hutchison W. Ian Lipkin |
| author_facet | Kirsi S. Honkavuori H. L. Shivaprasad Thomas Briese Craig Street David L. Hirschberg Stephen K. Hutchison W. Ian Lipkin |
| author_sort | Kirsi S. Honkavuori |
| collection | DOAJ |
| description | To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008–2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus. |
| first_indexed | 2024-04-13T02:45:28Z |
| format | Article |
| id | doaj.art-fdedb7f6a1c34516a1d5eed6226197e8 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-04-13T02:45:28Z |
| publishDate | 2011-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-fdedb7f6a1c34516a1d5eed6226197e82022-12-22T03:06:03ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592011-03-0117348048710.3201/eid1703.101410Novel Picornavirus in Turkey Poults with Hepatitis, California, USAKirsi S. HonkavuoriH. L. ShivaprasadThomas BrieseCraig StreetDavid L. HirschbergStephen K. HutchisonW. Ian LipkinTo identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008–2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus.https://wwwnc.cdc.gov/eid/article/17/3/10-1410_articleturkeysavianhepatitispicornavirusimmunohistochemistryviruses |
| spellingShingle | Kirsi S. Honkavuori H. L. Shivaprasad Thomas Briese Craig Street David L. Hirschberg Stephen K. Hutchison W. Ian Lipkin Novel Picornavirus in Turkey Poults with Hepatitis, California, USA Emerging Infectious Diseases turkeys avian hepatitis picornavirus immunohistochemistry viruses |
| title | Novel Picornavirus in Turkey Poults with Hepatitis, California, USA |
| title_full | Novel Picornavirus in Turkey Poults with Hepatitis, California, USA |
| title_fullStr | Novel Picornavirus in Turkey Poults with Hepatitis, California, USA |
| title_full_unstemmed | Novel Picornavirus in Turkey Poults with Hepatitis, California, USA |
| title_short | Novel Picornavirus in Turkey Poults with Hepatitis, California, USA |
| title_sort | novel picornavirus in turkey poults with hepatitis california usa |
| topic | turkeys avian hepatitis picornavirus immunohistochemistry viruses |
| url | https://wwwnc.cdc.gov/eid/article/17/3/10-1410_article |
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