Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds
The antiproliferative effect and mediation of apoptosis in human hepatoma HepG2 cells induced by djulis husk and its bioactive compounds was investigated. The ethanolic extracts of djulis husk (EEDH) at 50, 250, and 500 µg/mL induced remarkable cytotoxicity on HepG2 cells. By flow cytometry analysis...
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MDPI AG
2020-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/10/1514 |
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| author | Dom-Gene Tu Charng-Cherng Chyau Shih-Ying Chen Heuy-Ling Chu Shu-Chen Wang Pin-Der Duh |
| author_facet | Dom-Gene Tu Charng-Cherng Chyau Shih-Ying Chen Heuy-Ling Chu Shu-Chen Wang Pin-Der Duh |
| author_sort | Dom-Gene Tu |
| collection | DOAJ |
| description | The antiproliferative effect and mediation of apoptosis in human hepatoma HepG2 cells induced by djulis husk and its bioactive compounds was investigated. The ethanolic extracts of djulis husk (EEDH) at 50, 250, and 500 µg/mL induced remarkable cytotoxicity on HepG2 cells. By flow cytometry analysis, EEDH slowed down the cell cycle at the Sub-G0 phase after 24 h of incubation. Moreover, all EEDH treatment induced an apoptotic response in HepG2 cells. EEDH-induced apoptosis was associated with the attenuation of mitochondrial transmembrane potentials (ΔΨ<sub>m</sub>), an increase in Bax/Bcl-2 ratio, activation of caspase-3, and poly(ADP-ribose)polymerase (PARP) cleavage, as well as an increase in reactive oxygen species (ROS) generation. According to the HPLC-DAD and HPLC-MS/MS analysis, quercetin and kaempferol derivatives and another sixteen compounds were present in EEDH. Quercetin and kaempferol at 25–150 μM showed antiproliferative action and induced apoptosis on HepG2 cells, which may in part account for the anticancer activity of EEDH. Overall, EEDH may be a potent chemopreventive agent due to apoptosis in HepG2 cells. |
| first_indexed | 2024-03-10T15:27:09Z |
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| id | doaj.art-fdee18add338498bb3d714ca2de1eb91 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:27:09Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fdee18add338498bb3d714ca2de1eb912023-11-20T17:59:26ZengMDPI AGFoods2304-81582020-10-01910151410.3390/foods9101514Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive CompoundsDom-Gene Tu0Charng-Cherng Chyau1Shih-Ying Chen2Heuy-Ling Chu3Shu-Chen Wang4Pin-Der Duh5Department of Nuclear Medicine, Ditmanson Medical Foundation, Chia-Yi Christian Hospital, Chia-Yi 600, TaiwanResearch Institute of Biotechnology, Hungkuang University, Taichung 43302, TaiwanDepartment of Health and Nutrition, Chia Nan University of Pharmacy and Science, Tainan 71710, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, Tainan 71710, TaiwanThe antiproliferative effect and mediation of apoptosis in human hepatoma HepG2 cells induced by djulis husk and its bioactive compounds was investigated. The ethanolic extracts of djulis husk (EEDH) at 50, 250, and 500 µg/mL induced remarkable cytotoxicity on HepG2 cells. By flow cytometry analysis, EEDH slowed down the cell cycle at the Sub-G0 phase after 24 h of incubation. Moreover, all EEDH treatment induced an apoptotic response in HepG2 cells. EEDH-induced apoptosis was associated with the attenuation of mitochondrial transmembrane potentials (ΔΨ<sub>m</sub>), an increase in Bax/Bcl-2 ratio, activation of caspase-3, and poly(ADP-ribose)polymerase (PARP) cleavage, as well as an increase in reactive oxygen species (ROS) generation. According to the HPLC-DAD and HPLC-MS/MS analysis, quercetin and kaempferol derivatives and another sixteen compounds were present in EEDH. Quercetin and kaempferol at 25–150 μM showed antiproliferative action and induced apoptosis on HepG2 cells, which may in part account for the anticancer activity of EEDH. Overall, EEDH may be a potent chemopreventive agent due to apoptosis in HepG2 cells.https://www.mdpi.com/2304-8158/9/10/1514antiproliferationapoptosisdjulis huskquercetinkaempferol |
| spellingShingle | Dom-Gene Tu Charng-Cherng Chyau Shih-Ying Chen Heuy-Ling Chu Shu-Chen Wang Pin-Der Duh Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds Foods antiproliferation apoptosis djulis husk quercetin kaempferol |
| title | Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds |
| title_full | Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds |
| title_fullStr | Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds |
| title_full_unstemmed | Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds |
| title_short | Antiproliferative Effect and Mediation of Apoptosis in Human Hepatoma HepG2 Cells Induced by Djulis Husk and Its Bioactive Compounds |
| title_sort | antiproliferative effect and mediation of apoptosis in human hepatoma hepg2 cells induced by djulis husk and its bioactive compounds |
| topic | antiproliferation apoptosis djulis husk quercetin kaempferol |
| url | https://www.mdpi.com/2304-8158/9/10/1514 |
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