Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis
Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versi...
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7496 |
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| author | Isabel Olmos Calvo Olga Kuten-Pella Karina Kramer Ágnes Madár Szilvia Takács Dorottya Kardos Diána Simon Szabina Erdö-Bonyár Timea Berki Andrea De Luna Stefan Nehrer Zsombor Lacza |
| author_facet | Isabel Olmos Calvo Olga Kuten-Pella Karina Kramer Ágnes Madár Szilvia Takács Dorottya Kardos Diána Simon Szabina Erdö-Bonyár Timea Berki Andrea De Luna Stefan Nehrer Zsombor Lacza |
| author_sort | Isabel Olmos Calvo |
| collection | DOAJ |
| description | Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher <i>Col1a1</i> expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration. |
| first_indexed | 2024-03-10T09:37:50Z |
| format | Article |
| id | doaj.art-fdeeebca556940e982a59e6f6f020724 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:37:50Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fdeeebca556940e982a59e6f6f0207242023-11-22T03:59:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214749610.3390/ijms22147496Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in OsteoarthritisIsabel Olmos Calvo0Olga Kuten-Pella1Karina Kramer2Ágnes Madár3Szilvia Takács4Dorottya Kardos5Diána Simon6Szabina Erdö-Bonyár7Timea Berki8Andrea De Luna9Stefan Nehrer10Zsombor Lacza11OrthoSera GmbH, Dr. Karl-Dorrek-Straße 23–29, 3500 Krems an der Donau, AustriaOrthoSera GmbH, Dr. Karl-Dorrek-Straße 23–29, 3500 Krems an der Donau, AustriaCenter for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, AustriaOrthoSera GmbH, Dr. Karl-Dorrek-Straße 23–29, 3500 Krems an der Donau, AustriaOrthoSera GmbH, Dr. Karl-Dorrek-Straße 23–29, 3500 Krems an der Donau, AustriaResearch Center for Natural Sciences, 1117 Budapest, HungaryDepartment of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, HungaryDepartment of Immunology and Biotechnology, Medical School, University of Pécs, 7624 Pécs, HungaryCenter for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, AustriaCenter for Regenerative Medicine, Danube University of Krems, 3500 Krems an der Donau, AustriaDepartment of Sport Physiology, University of Physical Education, 1123 Budapest, HungaryHyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher <i>Col1a1</i> expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration.https://www.mdpi.com/1422-0067/22/14/7496hyperacute serumosteoarthritisjoint regenerationexplant co-cultureblood derived productinflammatory cytokines |
| spellingShingle | Isabel Olmos Calvo Olga Kuten-Pella Karina Kramer Ágnes Madár Szilvia Takács Dorottya Kardos Diána Simon Szabina Erdö-Bonyár Timea Berki Andrea De Luna Stefan Nehrer Zsombor Lacza Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis International Journal of Molecular Sciences hyperacute serum osteoarthritis joint regeneration explant co-culture blood derived product inflammatory cytokines |
| title | Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis |
| title_full | Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis |
| title_fullStr | Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis |
| title_full_unstemmed | Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis |
| title_short | Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis |
| title_sort | optimization of lyophilized hyperacute serum has as a regenerative therapeutic in osteoarthritis |
| topic | hyperacute serum osteoarthritis joint regeneration explant co-culture blood derived product inflammatory cytokines |
| url | https://www.mdpi.com/1422-0067/22/14/7496 |
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