A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores
Abstract Coccolithophores are globally abundant, calcifying microalgae that have profound effects on marine biogeochemical cycles, the climate, and life in the oceans. They are characterized by a cell wall of CaCO3 scales called coccoliths, which may contribute to their ecological success. The intri...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-39336-1 |
| _version_ | 1797795621211471872 |
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| author | Alastair Skeffington Axel Fischer Sanja Sviben Magdalena Brzezinka Michał Górka Luca Bertinetti Christian Woehle Bruno Huettel Alexander Graf André Scheffel |
| author_facet | Alastair Skeffington Axel Fischer Sanja Sviben Magdalena Brzezinka Michał Górka Luca Bertinetti Christian Woehle Bruno Huettel Alexander Graf André Scheffel |
| author_sort | Alastair Skeffington |
| collection | DOAJ |
| description | Abstract Coccolithophores are globally abundant, calcifying microalgae that have profound effects on marine biogeochemical cycles, the climate, and life in the oceans. They are characterized by a cell wall of CaCO3 scales called coccoliths, which may contribute to their ecological success. The intricate morphologies of coccoliths are of interest for biomimetic materials synthesis. Despite the global impact of coccolithophore calcification, we know little about the molecular machinery underpinning coccolithophore biology. Working on the model Emiliania huxleyi, a globally distributed bloom-former, we deploy a range of proteomic strategies to identify coccolithogenesis-related proteins. These analyses are supported by a new genome, with gene models derived from long-read transcriptome sequencing, which revealed many novel proteins specific to the calcifying haptophytes. Our experiments provide insights into proteins involved in various aspects of coccolithogenesis. Our improved genome, complemented with transcriptomic and proteomic data, constitutes a new resource for investigating fundamental aspects of coccolithophore biology. |
| first_indexed | 2024-03-13T03:20:46Z |
| format | Article |
| id | doaj.art-fdefe3f5d4384e32973224c88c62c05b |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T03:20:46Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fdefe3f5d4384e32973224c88c62c05b2023-06-25T11:22:19ZengNature PortfolioNature Communications2041-17232023-06-0114111510.1038/s41467-023-39336-1A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophoresAlastair Skeffington0Axel Fischer1Sanja Sviben2Magdalena Brzezinka3Michał Górka4Luca Bertinetti5Christian Woehle6Bruno Huettel7Alexander Graf8André Scheffel9Max-Planck Institute of Molecular Plant PhysiologyMax-Planck Institute of Molecular Plant PhysiologyMax-Planck Institute of Molecular Plant PhysiologyMax-Planck Institute of Molecular Plant PhysiologyMax-Planck Institute of Molecular Plant PhysiologyMax Planck Institute of Colloids and InterfacesMax Planck Institute for Plant Breeding Research, Max Planck-Genome-Centre CologneMax Planck Institute for Plant Breeding Research, Max Planck-Genome-Centre CologneMax-Planck Institute of Molecular Plant PhysiologyTechnische Universität Dresden, Faculty of BiologyAbstract Coccolithophores are globally abundant, calcifying microalgae that have profound effects on marine biogeochemical cycles, the climate, and life in the oceans. They are characterized by a cell wall of CaCO3 scales called coccoliths, which may contribute to their ecological success. The intricate morphologies of coccoliths are of interest for biomimetic materials synthesis. Despite the global impact of coccolithophore calcification, we know little about the molecular machinery underpinning coccolithophore biology. Working on the model Emiliania huxleyi, a globally distributed bloom-former, we deploy a range of proteomic strategies to identify coccolithogenesis-related proteins. These analyses are supported by a new genome, with gene models derived from long-read transcriptome sequencing, which revealed many novel proteins specific to the calcifying haptophytes. Our experiments provide insights into proteins involved in various aspects of coccolithogenesis. Our improved genome, complemented with transcriptomic and proteomic data, constitutes a new resource for investigating fundamental aspects of coccolithophore biology.https://doi.org/10.1038/s41467-023-39336-1 |
| spellingShingle | Alastair Skeffington Axel Fischer Sanja Sviben Magdalena Brzezinka Michał Górka Luca Bertinetti Christian Woehle Bruno Huettel Alexander Graf André Scheffel A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores Nature Communications |
| title | A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| title_full | A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| title_fullStr | A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| title_full_unstemmed | A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| title_short | A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| title_sort | joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores |
| url | https://doi.org/10.1038/s41467-023-39336-1 |
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