Acquisition and Analysis of Microcirculation Image in Septic Model Rats
<b>Background</b>: Microcirculation is a vital sign that supplies oxygen and nutrients to maintain normal life activities. Sepsis typically influences the operation of microcirculation, which is recovered by the administration of medicine injection. <b>Objective</b>: Sepsis-i...
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MDPI AG
2022-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/21/8471 |
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| author | Chen Ye Mami Kawasaki Kazuya Nakano Takashi Ohnishi Eizo Watanabe Shigeto Oda Taka-Aki Nakada Hideaki Haneishi |
| author_facet | Chen Ye Mami Kawasaki Kazuya Nakano Takashi Ohnishi Eizo Watanabe Shigeto Oda Taka-Aki Nakada Hideaki Haneishi |
| author_sort | Chen Ye |
| collection | DOAJ |
| description | <b>Background</b>: Microcirculation is a vital sign that supplies oxygen and nutrients to maintain normal life activities. Sepsis typically influences the operation of microcirculation, which is recovered by the administration of medicine injection. <b>Objective</b>: Sepsis-induced variation and recovery of microcirculation are quantitatively detected using microcirculation images acquired by a non-contact imaging setup, which might assist the clinical diagnosis and therapy of sepsis. <b>Methods</b>: In this study, a non-contact imaging setup was first used to record images of microcirculation on the back of model rats. Specifically, the model rats were divided into three groups: (i) the sham group as a control group; (ii) the cecum ligation and puncture (CLP) group with sepsis; and (iii) the CLP+thrombomodulin (TM) group with sepsis and the application of TM alfa therapy. Furthermore, considering the sparsity of red blood cells (RBCs), the blood velocity is estimated by robust principal component analysis (RPCA) and U-net, and the blood vessel diameter is estimated by the contrast difference between the blood vessel and tissue. <b>Results and Effectiveness</b>: In the experiments, the continuous degradation of the estimated blood velocity and blood vessel diameter in the CLP group and the recovery after degradation of those in the CLP+TM group were quantitatively observed. The variation tendencies of the estimated blood velocity and blood vessel diameter in each group suggested the effects of sepsis and its corresponding therapy. |
| first_indexed | 2024-03-09T18:40:40Z |
| format | Article |
| id | doaj.art-fdf55ed1a343434894d2e9e7552d6d15 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T18:40:40Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-fdf55ed1a343434894d2e9e7552d6d152023-11-24T06:48:34ZengMDPI AGSensors1424-82202022-11-012221847110.3390/s22218471Acquisition and Analysis of Microcirculation Image in Septic Model RatsChen Ye0Mami Kawasaki1Kazuya Nakano2Takashi Ohnishi3Eizo Watanabe4Shigeto Oda5Taka-Aki Nakada6Hideaki Haneishi7Center for Frontier Medical Engineering, Chiba University, Chiba 263-8522, JapanGraduate School of Science and Engineering, Chiba University, Chiba 263-8522, JapanFaculty of Science and Technology, Seikei University, Tokyo 180-8633, JapanMemorial Sloan Kettering Cancer Center, New York, NY 10065, USADepartment of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, JapanDepartment of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, JapanDepartment of Emergency and Critical Care Medicine, Graduate School of Medicine, Chiba University, Chiba 263-8522, JapanCenter for Frontier Medical Engineering, Chiba University, Chiba 263-8522, Japan<b>Background</b>: Microcirculation is a vital sign that supplies oxygen and nutrients to maintain normal life activities. Sepsis typically influences the operation of microcirculation, which is recovered by the administration of medicine injection. <b>Objective</b>: Sepsis-induced variation and recovery of microcirculation are quantitatively detected using microcirculation images acquired by a non-contact imaging setup, which might assist the clinical diagnosis and therapy of sepsis. <b>Methods</b>: In this study, a non-contact imaging setup was first used to record images of microcirculation on the back of model rats. Specifically, the model rats were divided into three groups: (i) the sham group as a control group; (ii) the cecum ligation and puncture (CLP) group with sepsis; and (iii) the CLP+thrombomodulin (TM) group with sepsis and the application of TM alfa therapy. Furthermore, considering the sparsity of red blood cells (RBCs), the blood velocity is estimated by robust principal component analysis (RPCA) and U-net, and the blood vessel diameter is estimated by the contrast difference between the blood vessel and tissue. <b>Results and Effectiveness</b>: In the experiments, the continuous degradation of the estimated blood velocity and blood vessel diameter in the CLP group and the recovery after degradation of those in the CLP+TM group were quantitatively observed. The variation tendencies of the estimated blood velocity and blood vessel diameter in each group suggested the effects of sepsis and its corresponding therapy.https://www.mdpi.com/1424-8220/22/21/8471microcirculationsepsisthrombomodulin (TM) alfablood velocityvessel diameterrobust principal component analysis (RPCA) |
| spellingShingle | Chen Ye Mami Kawasaki Kazuya Nakano Takashi Ohnishi Eizo Watanabe Shigeto Oda Taka-Aki Nakada Hideaki Haneishi Acquisition and Analysis of Microcirculation Image in Septic Model Rats Sensors microcirculation sepsis thrombomodulin (TM) alfa blood velocity vessel diameter robust principal component analysis (RPCA) |
| title | Acquisition and Analysis of Microcirculation Image in Septic Model Rats |
| title_full | Acquisition and Analysis of Microcirculation Image in Septic Model Rats |
| title_fullStr | Acquisition and Analysis of Microcirculation Image in Septic Model Rats |
| title_full_unstemmed | Acquisition and Analysis of Microcirculation Image in Septic Model Rats |
| title_short | Acquisition and Analysis of Microcirculation Image in Septic Model Rats |
| title_sort | acquisition and analysis of microcirculation image in septic model rats |
| topic | microcirculation sepsis thrombomodulin (TM) alfa blood velocity vessel diameter robust principal component analysis (RPCA) |
| url | https://www.mdpi.com/1424-8220/22/21/8471 |
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