Bridging PCR: An Efficient and Reliable Scheme Implemented for Genome-Walking

The efficacy of the available genome-walking methods is restricted by low specificity, high background, or composite operations. We herein conceived bridging PCR, an efficient genome-walking approach. Three primers with random sequences, inner walker primer (IWP), bridging primer (BP), and outer wal...

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Bibliographic Details
Main Authors: Zhiyu Lin, Cheng Wei, Jinfeng Pei, Haixing Li
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Current Issues in Molecular Biology
Subjects:
Online Access:https://www.mdpi.com/1467-3045/45/1/33