<i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells
Abnormal glycosylation of cancer cells is considered a key factor of carcinogenesis related to growth, proliferation, migration and invasion of tumor cells. Many plant-based polyphenolic compounds reveal potential anti-cancer properties effecting cellular signaling systems. Herein, we assessed the e...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8602 |
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| author | Iwona Radziejewska Katarzyna Supruniuk Michał Tomczyk Wiktoria Izdebska Małgorzata Borzym-Kluczyk Anna Bielawska Krzysztof Bielawski Anna Galicka |
| author_facet | Iwona Radziejewska Katarzyna Supruniuk Michał Tomczyk Wiktoria Izdebska Małgorzata Borzym-Kluczyk Anna Bielawska Krzysztof Bielawski Anna Galicka |
| author_sort | Iwona Radziejewska |
| collection | DOAJ |
| description | Abnormal glycosylation of cancer cells is considered a key factor of carcinogenesis related to growth, proliferation, migration and invasion of tumor cells. Many plant-based polyphenolic compounds reveal potential anti-cancer properties effecting cellular signaling systems. Herein, we assessed the effects of phenolic acid, <i>p</i>-coumaric acid and flavonoids such as kaempferol, astragalin or tiliroside on expression of selected cancer-related glycoforms and enzymes involved in their formation in AGS gastric cancer cells. The cells were treated with 80 and 160 µM of the compounds. RT-PCR, Western blotting and ELISA tests were performed to determine the influence of polyphenolics on analyzed factors. All the examined compounds inhibited the expression of MUC1, ST6GalNAcT2 and FUT4 mRNAs. C1GalT1, St3Gal-IV and FUT4 proteins as well as MUC1 domain, Tn and sialyl T antigen detected in cell lysates were also lowered. Both concentrations of kaempferol, astragalin and tiliroside also suppressed ppGalNAcT2 and C1GalT1 mRNAs. MUC1 cytoplasmic domain, sialyl Tn, T antigens in cell lysates and sialyl T in culture medium were inhibited only by kaempferol and tiliroside. Nuclear factor NF-κB mRNA expression decreased after treatment with both concentrations of kaempferol, astragalin and tiliroside. NF-κB protein expression was inhibited by kaempferol and tiliroside. The results indicate the rationality of application of examined polyphenolics as potential preventive agents against gastric cancer development. |
| first_indexed | 2024-03-09T05:21:47Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:21:47Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fdfd6bf55f274765ab92146dadcabead2023-12-03T12:40:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315860210.3390/ijms23158602<i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer CellsIwona Radziejewska0Katarzyna Supruniuk1Michał Tomczyk2Wiktoria Izdebska3Małgorzata Borzym-Kluczyk4Anna Bielawska5Krzysztof Bielawski6Anna Galicka7Department of Medical Chemistry, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandDepartment of Medical Chemistry, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandDepartment of Medical Chemistry, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandDepartment of Pharmaceutical Biochemistry, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandDepartment of Biotechnology, Faculty of Pharmacy, Medical University of Białystok, ul. Kilińskiego 1, 15-089 Białystok, PolandDepartment of Synthesis and Technology of Drugs, Faculty of Pharmacy, Medical University of Białystok, ul. Kilińskiego 1, 15-089 Białystok, PolandDepartment of Medical Chemistry, Faculty of Pharmacy, Medical University of Białystok, ul. Mickiewicza 2a, 15-222 Białystok, PolandAbnormal glycosylation of cancer cells is considered a key factor of carcinogenesis related to growth, proliferation, migration and invasion of tumor cells. Many plant-based polyphenolic compounds reveal potential anti-cancer properties effecting cellular signaling systems. Herein, we assessed the effects of phenolic acid, <i>p</i>-coumaric acid and flavonoids such as kaempferol, astragalin or tiliroside on expression of selected cancer-related glycoforms and enzymes involved in their formation in AGS gastric cancer cells. The cells were treated with 80 and 160 µM of the compounds. RT-PCR, Western blotting and ELISA tests were performed to determine the influence of polyphenolics on analyzed factors. All the examined compounds inhibited the expression of MUC1, ST6GalNAcT2 and FUT4 mRNAs. C1GalT1, St3Gal-IV and FUT4 proteins as well as MUC1 domain, Tn and sialyl T antigen detected in cell lysates were also lowered. Both concentrations of kaempferol, astragalin and tiliroside also suppressed ppGalNAcT2 and C1GalT1 mRNAs. MUC1 cytoplasmic domain, sialyl Tn, T antigens in cell lysates and sialyl T in culture medium were inhibited only by kaempferol and tiliroside. Nuclear factor NF-κB mRNA expression decreased after treatment with both concentrations of kaempferol, astragalin and tiliroside. NF-κB protein expression was inhibited by kaempferol and tiliroside. The results indicate the rationality of application of examined polyphenolics as potential preventive agents against gastric cancer development.https://www.mdpi.com/1422-0067/23/15/8602gastric cancerglycosylationMUC1polyphenolics |
| spellingShingle | Iwona Radziejewska Katarzyna Supruniuk Michał Tomczyk Wiktoria Izdebska Małgorzata Borzym-Kluczyk Anna Bielawska Krzysztof Bielawski Anna Galicka <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells International Journal of Molecular Sciences gastric cancer glycosylation MUC1 polyphenolics |
| title | <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells |
| title_full | <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells |
| title_fullStr | <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells |
| title_full_unstemmed | <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells |
| title_short | <i>p</i>-Coumaric acid, Kaempferol, Astragalin and Tiliroside Influence the Expression of Glycoforms in AGS Gastric Cancer Cells |
| title_sort | i p i coumaric acid kaempferol astragalin and tiliroside influence the expression of glycoforms in ags gastric cancer cells |
| topic | gastric cancer glycosylation MUC1 polyphenolics |
| url | https://www.mdpi.com/1422-0067/23/15/8602 |
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