<i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia
Reproductive disorders, presumably caused by <i>Chlamydia abortus</i>, are common among the ovine population of the Mari El Republic, Russia. <i>C. abortus</i> infection was determined by serologic testing or isolation and detection of the organism by PCR and direct immunoflu...
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MDPI AG
2022-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/12/1408 |
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| author | Irina Matveeva Nikolai Nikitin Ekaterina Evtushenko Karim Azimov Alexey Zaberezhny Olesya Bogomolova Maria Kruglova Vladimir Yeremets Evgeniya Markova Natalia Yeremets |
| author_facet | Irina Matveeva Nikolai Nikitin Ekaterina Evtushenko Karim Azimov Alexey Zaberezhny Olesya Bogomolova Maria Kruglova Vladimir Yeremets Evgeniya Markova Natalia Yeremets |
| author_sort | Irina Matveeva |
| collection | DOAJ |
| description | Reproductive disorders, presumably caused by <i>Chlamydia abortus</i>, are common among the ovine population of the Mari El Republic, Russia. <i>C. abortus</i> infection was determined by serologic testing or isolation and detection of the organism by PCR and direct immunofluorescence in tissue samples. Rams, ewes, and lambs (10 individuals each) were randomly chosen for serological testing by the complement fixation test and 7 of 30 (23%) animals tested were positive. Tissue samples were collected from ewes and aborted fetuses for isolation by inoculating chicken embryo yolk sacs (<i>n</i> = 41). The same samples were analyzed by PCR using commercial and in-house PCR kits and by direct immunofluorescence. <i>C. abortus</i> was detected in 58.5% of samples using PCR and in 60.9% of the samples by direct immunofluorescence. Five <i>Chlamydia</i> isolates were cultured in egg yolk sacs and adapted for growth in cell cultures. Phylogenetic analysis showed no substantial difference between Russian isolates and those from other parts of the world. The results of the study further demonstrate the usefulness of PCR for detection of <i>C. abortus</i> as a faster, simpler, and more reliable approach in comparison to culturing the organism and underscoring the necessity of screening for chlamydiosis as a cause of ovine abortion. |
| first_indexed | 2024-03-09T15:59:54Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T15:59:54Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fe0091c1523c481ea446a4677132591b2023-11-24T17:12:10ZengMDPI AGPathogens2076-08172022-11-011112140810.3390/pathogens11121408<i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of RussiaIrina Matveeva0Nikolai Nikitin1Ekaterina Evtushenko2Karim Azimov3Alexey Zaberezhny4Olesya Bogomolova5Maria Kruglova6Vladimir Yeremets7Evgeniya Markova8Natalia Yeremets9All-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaDepartment of Virology, Lomonosov Moscow State University, 119234 Moscow, RussiaDepartment of Virology, Lomonosov Moscow State University, 119234 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaAll-Russian Scientific Research and Technological Institute of Biological Industry, Biocombinat, 141142 Moscow, RussiaReproductive disorders, presumably caused by <i>Chlamydia abortus</i>, are common among the ovine population of the Mari El Republic, Russia. <i>C. abortus</i> infection was determined by serologic testing or isolation and detection of the organism by PCR and direct immunofluorescence in tissue samples. Rams, ewes, and lambs (10 individuals each) were randomly chosen for serological testing by the complement fixation test and 7 of 30 (23%) animals tested were positive. Tissue samples were collected from ewes and aborted fetuses for isolation by inoculating chicken embryo yolk sacs (<i>n</i> = 41). The same samples were analyzed by PCR using commercial and in-house PCR kits and by direct immunofluorescence. <i>C. abortus</i> was detected in 58.5% of samples using PCR and in 60.9% of the samples by direct immunofluorescence. Five <i>Chlamydia</i> isolates were cultured in egg yolk sacs and adapted for growth in cell cultures. Phylogenetic analysis showed no substantial difference between Russian isolates and those from other parts of the world. The results of the study further demonstrate the usefulness of PCR for detection of <i>C. abortus</i> as a faster, simpler, and more reliable approach in comparison to culturing the organism and underscoring the necessity of screening for chlamydiosis as a cause of ovine abortion.https://www.mdpi.com/2076-0817/11/12/1408chlamydiaovine enzootic abortionouter membrane protein A (OmpA)isolates |
| spellingShingle | Irina Matveeva Nikolai Nikitin Ekaterina Evtushenko Karim Azimov Alexey Zaberezhny Olesya Bogomolova Maria Kruglova Vladimir Yeremets Evgeniya Markova Natalia Yeremets <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia Pathogens chlamydia ovine enzootic abortion outer membrane protein A (OmpA) isolates |
| title | <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia |
| title_full | <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia |
| title_fullStr | <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia |
| title_full_unstemmed | <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia |
| title_short | <i>Chlamydia abortus</i> Isolation and Identification in Aborted Ovine Fetus in Mari El Republic of Russia |
| title_sort | i chlamydia abortus i isolation and identification in aborted ovine fetus in mari el republic of russia |
| topic | chlamydia ovine enzootic abortion outer membrane protein A (OmpA) isolates |
| url | https://www.mdpi.com/2076-0817/11/12/1408 |
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