Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material
Corneal disease has become the second leading cause of blindness in the world. Corneal transplantation is currently considered to be one of the common treatments for vision loss. This paper presents a novel approach utilizing dual-crosslinked membranes composed of polyrotaxane multiple aldehydes (PR...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/7/360 |
| _version_ | 1827732882561433600 |
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| author | Lulu Wang Yuehai Peng Wenfang Liu Li Ren |
| author_facet | Lulu Wang Yuehai Peng Wenfang Liu Li Ren |
| author_sort | Lulu Wang |
| collection | DOAJ |
| description | Corneal disease has become the second leading cause of blindness in the world. Corneal transplantation is currently considered to be one of the common treatments for vision loss. This paper presents a novel approach utilizing dual-crosslinked membranes composed of polyrotaxane multiple aldehydes (PRAs), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS) in the development process. Collagen was crosslinked, respectively, by EDC/NHS and PRAs to form stable amide bonds and imine groups. Through the formation of a double interpenetrating network, dual-crosslinked (Col-EDC-PRA) membranes exhibited enhanced resistance to collagenase degradation and superior mechanical properties compared to membranes crosslinked with a single crosslinker. Furthermore, Col-EDC-PRA membranes display favorable light transmittance and water content characteristics. Cell experiments showed that Col-EDC-PRA membranes were noncytotoxic and were not significantly different from other membranes. In a rabbit keratoplasty model, corneal stromal repair occurred at 5 months, evidenced by the presence of stromal cells and neo-stroma, as depicted in hematoxylin–eosin-stained histologic sections and optical coherence tomography images of the anterior segment. Moreover, there was no inflammation and corneal neovascularization, as well as no corneal rejection reaction in the surgical area. Overall, the results demonstrated that the dual-crosslinked membranes served effectively for corneal tissue regeneration after corneal defect. |
| first_indexed | 2024-03-11T00:57:15Z |
| format | Article |
| id | doaj.art-fe00b29264f741f4b1e7ecb7c0b93a41 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T00:57:15Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-fe00b29264f741f4b1e7ecb7c0b93a412023-11-18T19:56:22ZengMDPI AGJournal of Functional Biomaterials2079-49832023-07-0114736010.3390/jfb14070360Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair MaterialLulu Wang0Yuehai Peng1Wenfang Liu2Li Ren3Henan Provincial People’s Hospital, Henan Eye Hospital, Zhengzhou University People’s Hospital, Zhengzhou 450003, ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, ChinaCorneal disease has become the second leading cause of blindness in the world. Corneal transplantation is currently considered to be one of the common treatments for vision loss. This paper presents a novel approach utilizing dual-crosslinked membranes composed of polyrotaxane multiple aldehydes (PRAs), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS) in the development process. Collagen was crosslinked, respectively, by EDC/NHS and PRAs to form stable amide bonds and imine groups. Through the formation of a double interpenetrating network, dual-crosslinked (Col-EDC-PRA) membranes exhibited enhanced resistance to collagenase degradation and superior mechanical properties compared to membranes crosslinked with a single crosslinker. Furthermore, Col-EDC-PRA membranes display favorable light transmittance and water content characteristics. Cell experiments showed that Col-EDC-PRA membranes were noncytotoxic and were not significantly different from other membranes. In a rabbit keratoplasty model, corneal stromal repair occurred at 5 months, evidenced by the presence of stromal cells and neo-stroma, as depicted in hematoxylin–eosin-stained histologic sections and optical coherence tomography images of the anterior segment. Moreover, there was no inflammation and corneal neovascularization, as well as no corneal rejection reaction in the surgical area. Overall, the results demonstrated that the dual-crosslinked membranes served effectively for corneal tissue regeneration after corneal defect.https://www.mdpi.com/2079-4983/14/7/360collagen membranecorneal repaircorneal regeneration |
| spellingShingle | Lulu Wang Yuehai Peng Wenfang Liu Li Ren Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material Journal of Functional Biomaterials collagen membrane corneal repair corneal regeneration |
| title | Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material |
| title_full | Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material |
| title_fullStr | Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material |
| title_full_unstemmed | Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material |
| title_short | Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material |
| title_sort | properties of dual crosslinked collagen based membranes as corneal repair material |
| topic | collagen membrane corneal repair corneal regeneration |
| url | https://www.mdpi.com/2079-4983/14/7/360 |
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