Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux
The root of Aconitum carmichaelii Debeaux is used in Oriental folk medicine, but its cultivation is very labour intensive. Therefore, it is important to dissect the genetic mechanism of agronomic traits to breed elite cultivars for commercial cultivation. We analyzed the genetic diversity of 126 gen...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-09-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2017.1355747 |
| _version_ | 1828822506843144192 |
|---|---|
| author | Ma Yu Ling-Li Cao Yu-Xia Yang Ling-Liang Guan Lu-Lu Gou Xiao-Yan Shu Jing Huang Dan Liu Hong Zhang Da-Bin Hou |
| author_facet | Ma Yu Ling-Li Cao Yu-Xia Yang Ling-Liang Guan Lu-Lu Gou Xiao-Yan Shu Jing Huang Dan Liu Hong Zhang Da-Bin Hou |
| author_sort | Ma Yu |
| collection | DOAJ |
| description | The root of Aconitum carmichaelii Debeaux is used in Oriental folk medicine, but its cultivation is very labour intensive. Therefore, it is important to dissect the genetic mechanism of agronomic traits to breed elite cultivars for commercial cultivation. We analyzed the genetic diversity of 126 genotypes of A. carmichaelii collected from the major productive regions in China based on 280 amplified fragment length polymorphism (AFLP) loci. The values of the coefficient of gene differentiation, Nei's gene diversity index, and Shannon's information index, and the results from a hierarchical analysis of molecular variance analysis indicated that there is more genetic variability within populations than between populations. Marker–trait association analyses were conducted under a general linear model without controlling for population structure, and under a relative kinship model. In total, 59 loci were identified under both models for plant height (PH), stem diameter (SD), lateral root number (LRN), fresh weight and dry weight of stem (FWS and DWS, respectively), fresh weight and dry weight of leaves per plant (FWL and DWL, respectively), and fresh weight (FWR) and dry weight of individual lateral roots (DWR). Thirty-eight loci were associated with two to eight traits. For example, P1M1-39 was associated with variations in DWR, DWS, FWS, SD, DWL, FWL, FWR and PH, and P1M1-14 was close to a locus associated with variations in DWR, DWS, FWS, DWL, FWL, PH, FWR and LRN. Loci associated with favourable traits should be combined in commercial varieties to improve the yield and quality of A. carmichaelii. |
| first_indexed | 2024-12-12T13:16:21Z |
| format | Article |
| id | doaj.art-fe02457be57d44ae864292c2c906e828 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-12T13:16:21Z |
| publishDate | 2017-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-fe02457be57d44ae864292c2c906e8282022-12-22T00:23:25ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-09-0131590591110.1080/13102818.2017.13557471355747Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii DebeauxMa Yu0Ling-Li Cao1Yu-Xia Yang2Ling-Liang Guan3Lu-Lu Gou4Xiao-Yan Shu5Jing Huang6Dan Liu7Hong Zhang8Da-Bin Hou9Southwest University of Science and TechnologySouthwest University of Science and TechnologyInstitute of Traditional Chinese Medicine Resources and CultivationChinese Academy of Tropical Agricultural Sciences (CATAS), Tropical Crops Genetic Resources InstituteTriticeae Research Institute, Sichuan Agricultural UniversitySouthwest University of Science and TechnologySouthwest University of Science and TechnologySouthwest University of Science and TechnologySouthwest University of Science and TechnologySouthwest University of Science and TechnologyThe root of Aconitum carmichaelii Debeaux is used in Oriental folk medicine, but its cultivation is very labour intensive. Therefore, it is important to dissect the genetic mechanism of agronomic traits to breed elite cultivars for commercial cultivation. We analyzed the genetic diversity of 126 genotypes of A. carmichaelii collected from the major productive regions in China based on 280 amplified fragment length polymorphism (AFLP) loci. The values of the coefficient of gene differentiation, Nei's gene diversity index, and Shannon's information index, and the results from a hierarchical analysis of molecular variance analysis indicated that there is more genetic variability within populations than between populations. Marker–trait association analyses were conducted under a general linear model without controlling for population structure, and under a relative kinship model. In total, 59 loci were identified under both models for plant height (PH), stem diameter (SD), lateral root number (LRN), fresh weight and dry weight of stem (FWS and DWS, respectively), fresh weight and dry weight of leaves per plant (FWL and DWL, respectively), and fresh weight (FWR) and dry weight of individual lateral roots (DWR). Thirty-eight loci were associated with two to eight traits. For example, P1M1-39 was associated with variations in DWR, DWS, FWS, SD, DWL, FWL, FWR and PH, and P1M1-14 was close to a locus associated with variations in DWR, DWS, FWS, DWL, FWL, PH, FWR and LRN. Loci associated with favourable traits should be combined in commercial varieties to improve the yield and quality of A. carmichaelii.http://dx.doi.org/10.1080/13102818.2017.1355747Aconitum carmichaelii Debeauxagronomic traitgenetic diversitymarker–trait association |
| spellingShingle | Ma Yu Ling-Li Cao Yu-Xia Yang Ling-Liang Guan Lu-Lu Gou Xiao-Yan Shu Jing Huang Dan Liu Hong Zhang Da-Bin Hou Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux Biotechnology & Biotechnological Equipment Aconitum carmichaelii Debeaux agronomic trait genetic diversity marker–trait association |
| title | Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux |
| title_full | Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux |
| title_fullStr | Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux |
| title_full_unstemmed | Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux |
| title_short | Genetic diversity and marker–trait association analysis for agronomic traits in Aconitum carmichaelii Debeaux |
| title_sort | genetic diversity and marker trait association analysis for agronomic traits in aconitum carmichaelii debeaux |
| topic | Aconitum carmichaelii Debeaux agronomic trait genetic diversity marker–trait association |
| url | http://dx.doi.org/10.1080/13102818.2017.1355747 |
| work_keys_str_mv | AT mayu geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT linglicao geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT yuxiayang geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT lingliangguan geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT lulugou geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT xiaoyanshu geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT jinghuang geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT danliu geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT hongzhang geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux AT dabinhou geneticdiversityandmarkertraitassociationanalysisforagronomictraitsinaconitumcarmichaeliidebeaux |