Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei
Enterocytozoon hepatopenaei (EHP), the fungus-related micro-organism that infects the hepatopancreas of marine shrimp, is believed to cause growth retardation of the infected shrimp. As the hepatopancreatic cells are composed of different cell types that support the digestive system and innate def...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2022-02-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjst/journal/44-1/15.pdf |
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| author | Niroyhannah Nima Pornsawan Duangsuwan Pattira Pongtippatee Daungkhaetita Kanjanasopa Boonsirm Withyachumnarnkul |
| author_facet | Niroyhannah Nima Pornsawan Duangsuwan Pattira Pongtippatee Daungkhaetita Kanjanasopa Boonsirm Withyachumnarnkul |
| author_sort | Niroyhannah Nima |
| collection | DOAJ |
| description | Enterocytozoon hepatopenaei (EHP), the fungus-related micro-organism that infects the hepatopancreas of marine
shrimp, is believed to cause growth retardation of the infected shrimp. As the hepatopancreatic cells are composed of different
cell types that support the digestive system and innate defense, malfunction of the infected cells could lead to growth retardation
and weakness. This study aimed to determine of the percentage of each hepatopancreatic cell type infected by EHP and its
infectious levels in the normal and growth-retarded Pacific whiteleg shrimp Litopenaeus vannamei. Histological examination
revealed that all the cell types examined were infected with EHP, with significantly higher (p<0.01) percentage on the M- and Fcells, compared to other cell types. The R-, B- and F-cells of the growth-retarded shrimp were infected at significantly lower
(p<0.05) percentage than the same cell types of the normal shrimp. By PCR, positive reactions of EHP infection were detected in
all shrimp samples, both in the normal and growth-retarded shrimp, but only at the nested PCR level. Also, the relative density of
the EHP load in the hepatopancreas of the two shrimp groups did not differ statistically. These results suggest that growth
retardation in L. vannamei currently exists may not be due to EHP infection. |
| first_indexed | 2024-12-12T12:21:09Z |
| format | Article |
| id | doaj.art-fe05d543ea9e42d693458f69563a7f42 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-12T12:21:09Z |
| publishDate | 2022-02-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-fe05d543ea9e42d693458f69563a7f422022-12-22T00:24:39ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952022-02-014419710210.14456/sjst-psu.2022.15Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaeiNiroyhannah Nima0Pornsawan Duangsuwan1Pattira Pongtippatee2Daungkhaetita Kanjanasopa3Boonsirm Withyachumnarnkul4Department of Anatomy, Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandDepartment of Anatomy, Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112 ThailandFaculty of Innovative Agriculture and Fisheries Establishment Project, Prince of Songkla University, Surat Thani Campus, Mueang, Surat Thani, 84100 ThailandFaculty of Innovative Agriculture and Fisheries Establishment Project, Prince of Songkla University, Surat Thani Campus, Mueang, Surat Thani, 84100 ThailandFaculty of Innovative Agriculture and Fisheries Establishment Project, Prince of Songkla University, Surat Thani Campus, Mueang, Surat Thani, 84100 ThailandEnterocytozoon hepatopenaei (EHP), the fungus-related micro-organism that infects the hepatopancreas of marine shrimp, is believed to cause growth retardation of the infected shrimp. As the hepatopancreatic cells are composed of different cell types that support the digestive system and innate defense, malfunction of the infected cells could lead to growth retardation and weakness. This study aimed to determine of the percentage of each hepatopancreatic cell type infected by EHP and its infectious levels in the normal and growth-retarded Pacific whiteleg shrimp Litopenaeus vannamei. Histological examination revealed that all the cell types examined were infected with EHP, with significantly higher (p<0.01) percentage on the M- and Fcells, compared to other cell types. The R-, B- and F-cells of the growth-retarded shrimp were infected at significantly lower (p<0.05) percentage than the same cell types of the normal shrimp. By PCR, positive reactions of EHP infection were detected in all shrimp samples, both in the normal and growth-retarded shrimp, but only at the nested PCR level. Also, the relative density of the EHP load in the hepatopancreas of the two shrimp groups did not differ statistically. These results suggest that growth retardation in L. vannamei currently exists may not be due to EHP infection.https://rdo.psu.ac.th/sjst/journal/44-1/15.pdfgrowth retardationwhite-feces diseaseshrimp cultureenterocytozoon hepatopenaeimicrosporidia |
| spellingShingle | Niroyhannah Nima Pornsawan Duangsuwan Pattira Pongtippatee Daungkhaetita Kanjanasopa Boonsirm Withyachumnarnkul Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei Songklanakarin Journal of Science and Technology (SJST) growth retardation white-feces disease shrimp culture enterocytozoon hepatopenaei microsporidia |
| title | Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei |
| title_full | Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei |
| title_fullStr | Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei |
| title_full_unstemmed | Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei |
| title_short | Types of cells in the hepatopancreas of the Pacific whiteleg shrimp Litopenaeus vannamei being infected by Enterocytozoon hepatopenaei |
| title_sort | types of cells in the hepatopancreas of the pacific whiteleg shrimp litopenaeus vannamei being infected by enterocytozoon hepatopenaei |
| topic | growth retardation white-feces disease shrimp culture enterocytozoon hepatopenaei microsporidia |
| url | https://rdo.psu.ac.th/sjst/journal/44-1/15.pdf |
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