Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach
Abstract This single cell study aimed to clarify whether an elevated incidence of sperm with a retained cytoplasmic droplet (CD) can be compensated by a higher sperm number in boar semen doses to maintain fertility. Cluster analysis of motile spermatozoa (ten boars) revealed that spermatozoa with a...
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| Format: | Article |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26020-5 |
| _version_ | 1798005315100213248 |
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| author | Heiko Henning Anne-Marie Luther Lisa Höfner-Schmiing Dagmar Waberski |
| author_facet | Heiko Henning Anne-Marie Luther Lisa Höfner-Schmiing Dagmar Waberski |
| author_sort | Heiko Henning |
| collection | DOAJ |
| description | Abstract This single cell study aimed to clarify whether an elevated incidence of sperm with a retained cytoplasmic droplet (CD) can be compensated by a higher sperm number in boar semen doses to maintain fertility. Cluster analysis of motile spermatozoa (ten boars) revealed that spermatozoa with a CD are underrepresented in the fast, linearly moving sperm cohort compared to morphologically normal sperm. Nonetheless, the response to the motility stimulator procaine was barely affected in spermatozoa with distal CD (Cramer’s V = 0.14), but moderately affected in sperm with proximal CD (V = 0.28). Viability was lower in sperm with distal CD (p < 0.05) but not with proximal CD compared to normal sperm during 168 h storage of extended semen samples (n = 11) and subsequent thermic stress. Morphologically normal sperm from normospermic samples (n = 10) or samples with a high incidence (≥ 15%) of sperm with CD (n = 9) had similar motility patterns and responses to procaine. The origin of morphologically normal sperm had no effect on sperm viability (p > 0.05; n = 26). In conclusion, a moderately enhanced prevalence of sperm with CD seems to be compensable by an increase in sperm numbers in boar semen doses. |
| first_indexed | 2024-04-11T12:37:15Z |
| format | Article |
| id | doaj.art-fe0b1494c52c42829e0bde0dec570300 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T12:37:15Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fe0b1494c52c42829e0bde0dec5703002022-12-22T04:23:35ZengNature PortfolioScientific Reports2045-23222022-12-0112111210.1038/s41598-022-26020-5Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approachHeiko Henning0Anne-Marie Luther1Lisa Höfner-Schmiing2Dagmar Waberski3Institute of Farm Animal Genetics, Friedrich-Loeffler-InstitutUnit for Reproductive Medicine / Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, FoundationUnit for Reproductive Medicine / Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, FoundationUnit for Reproductive Medicine / Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, FoundationAbstract This single cell study aimed to clarify whether an elevated incidence of sperm with a retained cytoplasmic droplet (CD) can be compensated by a higher sperm number in boar semen doses to maintain fertility. Cluster analysis of motile spermatozoa (ten boars) revealed that spermatozoa with a CD are underrepresented in the fast, linearly moving sperm cohort compared to morphologically normal sperm. Nonetheless, the response to the motility stimulator procaine was barely affected in spermatozoa with distal CD (Cramer’s V = 0.14), but moderately affected in sperm with proximal CD (V = 0.28). Viability was lower in sperm with distal CD (p < 0.05) but not with proximal CD compared to normal sperm during 168 h storage of extended semen samples (n = 11) and subsequent thermic stress. Morphologically normal sperm from normospermic samples (n = 10) or samples with a high incidence (≥ 15%) of sperm with CD (n = 9) had similar motility patterns and responses to procaine. The origin of morphologically normal sperm had no effect on sperm viability (p > 0.05; n = 26). In conclusion, a moderately enhanced prevalence of sperm with CD seems to be compensable by an increase in sperm numbers in boar semen doses.https://doi.org/10.1038/s41598-022-26020-5 |
| spellingShingle | Heiko Henning Anne-Marie Luther Lisa Höfner-Schmiing Dagmar Waberski Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach Scientific Reports |
| title | Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach |
| title_full | Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach |
| title_fullStr | Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach |
| title_full_unstemmed | Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach |
| title_short | Compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination: a single cell approach |
| title_sort | compensability of an enhanced incidence of spermatozoa with cytoplasmic droplets in boar semen for use in artificial insemination a single cell approach |
| url | https://doi.org/10.1038/s41598-022-26020-5 |
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